P. gingivalis 에 대한 피톤치드의 항균효과

F i g . 5 . S D S - P A G E a n a l y s i s o f P. gingivalis w i t h o r w i t h o u t p h y t o n c i d e . P.
gingivalis 2561 grown up to optical density of 0.4 at 600 ㎜ was further incubated with
0.005% phytoncide for 6 h. The bacterial cells were centrifuged to collect the cell pellet,
boiled in sample buffer for 10 min, and subjected to SDS-10% polyacrylamide gel
electrophoresis. Lanes: M, protein molecular markers; 1, P. gingivalis without
phytoncide; 2, P. gingivalis w it h 0.005 % p hy t o cid e .
F i g . 6 . Im m u n b l o t a n a l y s i s o f P. gingivalis w i t h o r w i t h o u t p h y t o n c i d e . The boiled
sample prepared in Fig. 5 was subjected to immunoblot. The separated proteins in the
sample by SDS-PAGE were transferred to a PVDF membrane. The membrane was
blocked and incubated anti-P. gingivalis whole cell or anti-fimbrillin antibodies and then
with secondary antibodies conjugated with alkaline phosphatase. Lanes: 1, P. gingivalis
without phytoncide; 2, P. gingivalis w it h 0.005 % p hy t o n cid e .
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