P. gingivalis 에 대한 피톤치드의 항균효과

사진 및 사진 설 명
F i g . 1 . A t r a n s m i s s i o n e l e c t r o n m i c r o p h o t o g r a p h o f P. gingivalis i n c u b a t e d
w i t h o u t p h y t o n c i d e . P. gingivalis 2561 grown up to optical density of 0.4 at 600 ㎜
was further incubated without phytoncide for 6 h. The bacterial cells were harvested,
fixed, embedded, ultrathin-sectioned, and then stained with uranyl acetate and lead
citrate. Notice that the inner and outer membranes of the cells are discernible and
ribosomes are scattered in the cytoplasm but more toward the cell membrane. Some
cells are undergoing cell division and have electron dense granules which are thought be
polyphosphate reserve materials. (X 40,000 mag.)
F i g . 2. A t r a n s m i s s i o n e l e c t r o n m i c r o p h o t o g r a p h o f P. gingivalis i n c u b a t e d w i t h
0.005 % p h y t o n c i d e . P. gingivalis 2561 grown up to optical density of 0.4 at 600 ㎜
was further incubated with 0.005% phytoncide for 6 h. Morphologically abnormal cells
are prominent, which have distinct nucleotide and more electron dense granules.
Ribosomes in the cytoplasm are densely located more toward the membrane. A few
lysed ghost cells with sparse remnant of the cell body are observed and abnormally
smaller sized, degenerated cells are also detected. (X 40,000 mag.)
F i g . 3 . A t r a n s m i s s i o n e l e c t r o n m i c r o p h o t o g r a p h o f P. gingivalis i n c u b a t e d w i t h
0.01 % p h y t o n c i d e . P. gingivalis 2561 grown up to optical density of 0.4 at 600 ㎜ was
further incubated with 0.01% phytoncide for 6 h. Note that occurrence of ghost cells
becomes prominent and numerous small-sized vesicles are scattered . ( X 4 0,000 m a g .)
F i g . 4 . C h a n g e i n e x p r e s s i o n o f s u p e r o x i d e d i s m u t a s e ( S O D ) m RN A o f P.
gingivalis a f t e r t h e a d d i t i o n o f p h y t o n c i d e a s d e t e r m i n e d b y RT- P C R. P.
gingivalis 2561 grown up to optical density of 0.4 at 600 ㎚ was further incubated with
0.005% phytoncide for 2 h. The cultured cells of P. gingivalis were centrifuged to
collect the cell pellet. Total RNA was extracted from the cell pellet and subjected to
RT-PCR to examine the expression of superoxide dismutase gene (sod ) and GapA
housekeeping gene (gap A) as a control. Note that the expression of so d was
dramatically decreased after the incubation with phytoncide.
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