P. gingivalis 에 대한 피톤치드의 항균효과

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- A b s t r a c t - E f f e c t o f p h y t o n c i d e o n Po r p h y r o m o nas gingivalis S u n - Q K i m , D.M.D., M.S.D. Department of Oral Medicine, Division of Dentistry, Graduate School, Kyung Hee University (Directed by Yang-Hyun Chun, D.M.D., M.S.D., Ph.D) Trees emit the phytoncide into atmosphere to protect them from predation. Phytoncide from different trees has its own unique fragrance that is referred to as forest bath. Phytoncide, which is essential oil of trees, has microbicidal, insecticidal, acaricidal, and deodorizing effect. The present study was performed to examine the effect of phytoncide on Porp hy rom onas gingivalis, which is one of the most important causative agents of periodontitis and halitosis. P. gingivalis 2561 was incubated with or without phytoncide extracted from Hinoki (C ham aec yp aris obtu sa Sieb. et Zucc.; Japanese cypress) and then changes were observed in its cell viability, antibiotic sensitivity, morphology, and biochemical/molecular biological pattern. The results were as follows: 1. The phytoncide appeared to have a strong antibacterial effect on P. gingivalis. MIC of phytoncide for the bacterium was determined to be 0.008%. The antibacterial effect was attributed to bactericidal activity against P. gingivalis. It almost completely suppressed the bacterial cell viability (>99.9%) at the concentration of 0.01%, which is the MBC for the bacterium. 2. The phytoncide failed to enhance the bacterial susceptibility to ampicillin, cefotaxime, penicillin, and tetracycline but did increase the susceptibility to amoxicillin. 3. Numbers of electron dense granules, ghost cell, and vesicles increased with increasing concentration of the phytoncide, 4. RT-PCR analysis revealed that expression of superoxide dismutase was increased in the bacterium incubated with the phytoncide. 5. No distinct difference in protein profile between the bacterium incubated with or - 18 -
without the phytoncide was observed as determined by SDS-PAGE and immunoblot. Overall results suggest that the phytoncide is a strong antibacterial agent that has a bactericidal action against P. gingivalis. The phytoncide does not seem to affect much the profile of the major outer membrane proteins but interferes with antioxidant activity of the bacterium. Along with this, yet unknown mechanism may cause changes in cell morphology and eventually cell death. - 19 -
Page 1 and 2: 박 사 학 위 논 문 P. gingival
Page 3 and 4: 김선규의 치의학 박사학위
Page 5 and 6: Ⅰ . 서 론 인간의 구강 내
Page 7 and 8: 실 험 균주의 배양 II. 실
Page 9 and 10: 하였다. 배양액에 피톤치
Page 11 and 12: P. gingivalis에 대한 피톤치
Page 13 and 14: P. gingivalis was grown in the pres
Page 15 and 16: 있 다. 편 백 피톤치드와
Page 17 and 18: 요 인에 대한 방 어 작용으
Page 19 and 20: 참 고 문 헌 1. Gharbia, S.E. an
Page 21: 28. Cox, S.D., Mann, C.M., Markham,
Page 25 and 26: F i g . 5 . S D S - P A G E a n a l
Page 27 and 28: F i g . 2 - 23 -
Page 29 and 30: F i g . 4 -phyton +phyton - 25 -
Page 31: F i g . 6 1 2 - 27 -

P. gingivalis 에 대한 피톤치드의 항균효과

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