P. gingivalis 에 대한 피톤치드의 항균효과

- A b s t r a c t -
E f f e c t o f p h y t o n c i d e o n Po r p h y r o m o nas gingivalis
S u n - Q K i m , D.M.D., M.S.D.
Department of Oral Medicine, Division of Dentistry, Graduate School, Kyung Hee
University
(Directed by Yang-Hyun Chun, D.M.D., M.S.D., Ph.D)
Trees emit the phytoncide into atmosphere to protect them from predation. Phytoncide
from different trees has its own unique fragrance that is referred to as forest bath.
Phytoncide, which is essential oil of trees, has microbicidal, insecticidal, acaricidal, and
deodorizing effect. The present study was performed to examine the effect of phytoncide
on Porp hy rom onas gingivalis, which is one of the most important causative agents of
periodontitis and halitosis. P. gingivalis 2561 was incubated with or without phytoncide
extracted from Hinoki (C ham aec yp aris obtu sa Sieb. et Zucc.; Japanese cypress) and then
changes were observed in its cell viability, antibiotic sensitivity, morphology, and
biochemical/molecular biological pattern. The results were as follows:
1. The phytoncide appeared to have a strong antibacterial effect on P. gingivalis. MIC
of phytoncide for the bacterium was determined to be 0.008%. The antibacterial effect
was attributed to bactericidal activity against P. gingivalis. It almost completely
suppressed the bacterial cell viability (>99.9%) at the concentration of 0.01%, which
is the MBC for the bacterium.
2. The phytoncide failed to enhance the bacterial susceptibility to ampicillin, cefotaxime,
penicillin, and tetracycline but did increase the susceptibility to amoxicillin.
3. Numbers of electron dense granules, ghost cell, and vesicles increased with increasing
concentration of the phytoncide,
4. RT-PCR analysis revealed that expression of superoxide dismutase was increased in
the bacterium incubated with the phytoncide.
5. No distinct difference in protein profile between the bacterium incubated with or
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