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A <strong>COMPARISON</strong> <strong>BETWEEN</strong> <strong>MCPA</strong> <strong>AND</strong> <strong>PESCO</strong> <strong>is</strong>-<strong>I5</strong> <strong>FOR</strong><br />

<strong>THE</strong> CONTROL OF WEEDS IN RICE ON <strong>THE</strong> ACCRAPLAINS<br />

By K. A. HAIZEL<br />

Faculty of Agriculture. University of Ghana, Legon<br />

Summary<br />

Rice cultivation has great possibilities of<br />

increasing production on the Accra Plains,<br />

especially on the heavy black clays, when<br />

water becomes available in large quantities<br />

for irrigation from the Akosombo dam.<br />

With expanding and intensive rice production<br />

weed control <strong>is</strong> likely to be one of<br />

the problems which would require attention.<br />

. Of the various weed control measures the<br />

use of herbicides <strong>is</strong> the most prom<strong>is</strong>ing.<br />

<strong>MCPA</strong>, now in common use in rice, was<br />

compared against Pesco 18-15 which by<br />

virtue of its composition should have a wider<br />

spectrum of weed control.<br />

Both herbicides proved highly toxic at<br />

pre-emergence application, but at postemergence<br />

<strong>MCPA</strong> showed better trend of<br />

efficiency and <strong>is</strong> likely to hold its sway even<br />

at much higher dosages.<br />

Introduction<br />

Rice production constitutes one of the<br />

major cropping possibilities of the Accra<br />

Plains. The Black clays of which there are<br />

about 319,000 acres have proved pre-eminently<br />

suitable for rice cultivation with irrigation<br />

at the University Farm at Kpong.<br />

When more water becomes available from the<br />

Akosombo dam the acreage of the crop<br />

could be increased considerably.<br />

An important aspect of intensive rice<br />

cultivation <strong>is</strong> the problem of keeping the<br />

crop weed free. In Ghana the problem could<br />

become even more pressing as rice <strong>is</strong><br />

seeded directly in the field and not transplanted.<br />

The crop therefore has no initial<br />

ad vantage over the weeds in the course of<br />

its establ<strong>is</strong>hment.<br />

<strong>AND</strong><br />

A. G. CARSON<br />

Agricultural Irrigation Research Station, Kade.<br />

Under irrigation, flooding <strong>is</strong> an adequate<br />

measure for the control of most weeds,<br />

particularly species that are normally adapted<br />

to dry land conditions. Other weed species,<br />

especially the aquatic types such as Sphenoclea<br />

zeylanica and Cyperus difform<strong>is</strong>, can<br />

be tough and are not so easily controlled<br />

by normal flooding.<br />

The use of mechanical means for the<br />

control of weeds in rice <strong>is</strong> not easy and<br />

often not quite practicable. Planting in rows<br />

allows mechanical after~cultivation to a<br />

limited extent, but close planting, as <strong>is</strong><br />

normally done for the crop, makes such a<br />

venture rather hazardous to the crop.<br />

Hand hoeing requires a lot of care because<br />

grassy weed species are often not easily<br />

d<strong>is</strong>tingu<strong>is</strong>hable from the rice seedling, particularly<br />

in the early stages of growth. When<br />

employed on a large scale, therefore, hand<br />

hoeing <strong>is</strong> likely to be slow, untimely, expensive<br />

and not thorough. The future of effective<br />

weed control in rice, therefore, seems to<br />

lie in the use of suitable selective herbicides.<br />

For some years <strong>MCPA</strong> and 2-4 D have<br />

been the most popular herbicides used in rice<br />

in most parts of the world. These two herbicides<br />

are of the translocated type and are<br />

effective mainly against broadleaved weeds.<br />

Under most situations, however, it <strong>is</strong> the<br />

grassy weeds that constitute a great problem.<br />

Recently, F<strong>is</strong>ons Pest Control put out a<br />

product, Pesco 18-15, which <strong>is</strong> claimed<br />

to be selective in rice in controlling both<br />

broadleaved weeds as well as grassy weed<br />

species. Pesco 18-15 <strong>is</strong>, in composition, a<br />

mixture of <strong>MCPA</strong> and TCA (Trichloro<br />

Acetic Acid). While <strong>MCPA</strong> <strong>is</strong> essentially.<br />

a broadleaved weed killer, TCA <strong>is</strong> mainly<br />

a grass killer. Pesco 18-15, being a cocktail<br />

of the two, should, therefore, have a broader<br />

spectrum of control, destroying broadleaved<br />

weeds as well as grassy species.<br />

Th<strong>is</strong> experiment was carried out to compare<br />

the relative effectiveness of <strong>MCPA</strong>,<br />

already in common use in rice, and Pesco<br />

18-15 the new product, in the control of<br />

weeds.


Materials and Methods<br />

The experiment was laid out on the Black<br />

Akuse Clay soils at Kpong.<br />

The land was ploughed, harrowed .and<br />

levelled, but was not puddled. Treatment<br />

plots (1/57.4 acre each) were enclosed in separate<br />

bunds to enable independent flooding.<br />

Paddy (variety Nickerie), treated with agrosan<br />

at the rate of 1 oz. to 28 lb. seed, was<br />

broadcast at the rate of 80 lb. per acre<br />

on 20th October, 1964. The' seed was not<br />

pre-germinated. The layout. of the experiment<br />

was a random<strong>is</strong>ed block design,<br />

replicated three times. The treatments<br />

cons<strong>is</strong>ted of two herbicides: MCP A and<br />

Pesco 18-15, applied at two levels each: three<br />

pts./ac. and five pts./ac, and applied at two<br />

different times: pre-emergence and post-emergence.<br />

The~e were compared against weeded<br />

and unweeded rice plots.<br />

The pre-emergence treatments were applied<br />

two days after sowing, on the 22nd October,<br />

1964. The post-emergence treatments were<br />

applied on 20th November, 1964 four weeks<br />

after sowing. The application of the herbicides<br />

was done with a pressur<strong>is</strong>ed napsac<br />

sprayer operating around a pressure of<br />

about 60 lb./sq. inch and at a volume of<br />

30 gallons per/acre. The plots with 'clean<br />

weeding' treatment were kept weed free<br />

by periodic removal of weeds by hand hoeing.<br />

Owing to the difficulty of d<strong>is</strong>tingu<strong>is</strong>hing<br />

the grassy weed species from the rice crop<br />

at the early stages, thorough weeding by<br />

hoeing was not possible until later. In the<br />

plots with 'no weeding' treatment weeds<br />

were allowed to grow with the crop freely<br />

throughout the experiment.<br />

Observations and D<strong>is</strong>cussion<br />

Germination.-Pre-emergence application<br />

of the herbicide delayed germination remarkably<br />

(see Table 1 below).<br />

TABLE 1<br />

EFFECT OF TREATMENTS ON <strong>THE</strong> GERMINATION OF PADDY<br />

I. <strong>MCPA</strong> at 3pts/ac. Pre-em.<br />

II. <strong>MCPA</strong> at 5pts/ac. Pre-em.<br />

III. Pesco 18-15 3pt/ac. Pre-em.<br />

IV. Pesco 18-15 5pt/ac. Pre-em.<br />

V. <strong>MCPA</strong> at 3pt./ac. Post-em.<br />

VI. <strong>MCPA</strong> at 5pt/ac. Post-em.<br />

VII. Pesco 18-15 3pt/ac. Post-em.<br />

VIII. Pesco 18-15 5pt/ac. Post-em.<br />

IX. Clean Weeding . . . .<br />

X. No Weeding<br />

1. Standard Error of the Mean=± 2.14<br />

2. Shortest Significant Ranges<br />

at 5% level<br />

p:<br />

(Rp)<br />

3. Treatment Means ranked in<br />

order<br />

(2)<br />

2.97<br />

6.35<br />

IV<br />

23.9<br />

Time of Germination<br />

(50 % Thro.)<br />

16 days<br />

16 days<br />

16 days<br />

16 days<br />

6 daysi<br />

6 days I<br />

6 days ~<br />

~~:~ I<br />

6 daysJ<br />

(3)<br />

3.12<br />

6.67<br />

I<br />

34.1<br />

(4)<br />

3.21<br />

6.87<br />

III<br />

36.2<br />

31.6<br />

10.0<br />

·35.0<br />

16.6<br />

(5)<br />

3.27<br />

7.00<br />

V<br />

71.6<br />

34.1<br />

18.0<br />

36.2<br />

23.9


The germination percentages for the treatments<br />

were transformed by the angular<br />

transformation (LeClerg et al. 1962) and<br />

subjected to an analys<strong>is</strong> of variance. At the<br />

time of assessment the post-emergence<br />

treatments had not been applied. For<br />

purposes of th<strong>is</strong> compar<strong>is</strong>on, therefore, all<br />

treatments other than pre-emergence application<br />

should be considered as untreated.<br />

The results of th<strong>is</strong> analys<strong>is</strong> indicated<br />

highly significant effects due to treatments.<br />

The untreated plots gave a uniform germinanation<br />

of about 90 per cent each, while the<br />

pre-emergence treated plots gave well below<br />

40 per cent germination. Within the preemergence<br />

treatment plots, there was no<br />

significant difference in effect due to <strong>MCPA</strong><br />

and Pesco 18-15 at the same dosage levels,<br />

i.e. at 5pts/acre or 3ptsjacre. There were,<br />

however, significant differences in effects due<br />

to the different dosage levels of application.<br />

At the higher dosage rate of 5ptsjacre of<br />

either <strong>MCPA</strong> or Pesco 18-15, there was a<br />

more significant depression in total germination.<br />

The drastic effect of the herbicide on<br />

germination <strong>is</strong> evidently due to the fact that<br />

the broadcast seeds were exposed to direct<br />

contact with the herbicide at the time of<br />

application.<br />

The effect of pre~emergence application <strong>is</strong><br />

further indicated by the total percentage<br />

germination for treated and untreated plots<br />

(Table 1 above). There was a considerable<br />

drop in the plots with the pre-emergence<br />

treated plots.<br />

TABLE 2<br />

Tillering.-Tillering was observed in all plots<br />

21 days after sowing, but the degree of<br />

tillering differed with the treated and untreated<br />

plots. The figures in Table 2 below<br />

sho~ the mean number of tillers per plant<br />

per plot. These were based on counts from<br />

10 plants taken at random in each plot.<br />

These counts were transformed by the<br />

square root transformation, using the<br />

formula YV+0.5 (LeClerg et al. 1962), and<br />

then subjected to an analys<strong>is</strong> of variance.<br />

Th<strong>is</strong> showed that differences due to treatment<br />

were just significant at the 5 per cent level.<br />

Further examination using Duncan's (1955)<br />

shortest significant range test revealed that the<br />

pre-emergence treatments significantly affected<br />

the degree of tillering in the crop. As the<br />

post-emergence application had not been<br />

applied at the time of assessment it was not<br />

strange that post-emergence treatments did<br />

not show any significant differences from the<br />

"weeded" and "unweeded plots".<br />

Flowering.-Flowering was delayed by preemergence<br />

application but post·emergence<br />

application 4 weeks after planting had no<br />

effect on the flowering period. The delay in<br />

flowering was about a week, being 108 days<br />

for the pre-emergence plots as against 101days<br />

for the post.,emergence, hand weeded and<br />

unweeded plots. .<br />

Crop Health.-The effect of the herbicide<br />

application on the general growth of the<br />

crop was assessed by guided v<strong>is</strong>ual scoring.<br />

The scale used <strong>is</strong> based on the one used by<br />

Green & Kaloger<strong>is</strong> (1963) and <strong>is</strong> given<br />

below as Table 3.<br />

EFFECf OF TREATMENT ON NUMBER OF TILLERS PER PLANT<br />

(a) Standard Error of the Mean- ± 0.28<br />

(b) Shortest SignificantRange (Rp)<br />

p.: (2) (3) (4) (5) (6) (7) (8) (9) (10)<br />

2.97 3.12 3.21 3.27 3.32 3.35 3.37 3.39 3.4<br />

Rp 0.83 0.87 0.90 0.92 0.93 0.94 0.94 0.95 0.9<br />

(c) Ranked Mean of Treatments<br />

Post-em Post-em Post-em Clean No Post-em Pre-em Pre-em Pre-em Pre-em<br />

Treatment<br />

Orig.<br />

<strong>MCPA</strong><br />

3ptjac<br />

3.7<br />

Pesco<br />

5ptjac<br />

3.7<br />

pesco<br />

3ptjac<br />

4.7<br />

Weeding<br />

4.7<br />

Weeding<br />

5.0<br />

<strong>MCPA</strong> Pesco Pesco<br />

5ptjac 5ptjac 3ptjac<br />

5.3 8.3 8.3<br />

Pesco <strong>MCPA</strong><br />

3ptjac 5ptjac<br />

8.3 11.0<br />

Transf. 2.0 2.0 2.2 2.2 2.3 2.4 2.8 2.9 2.9 3.4


TABLE 3<br />

CROP HEALTH SCALE<br />

Crop stand as healthy as that of weeded plot<br />

Slight stunting or delay in development<br />

Slight necros<strong>is</strong> of leaves, chloros<strong>is</strong> or reduced vigour of<br />

crop<br />

Considerable leaf scorch, chloros<strong>is</strong> or considerable reduction<br />

in vigour of crop.<br />

Crop severely damaged, stunted or deformed<br />

Crop killed.<br />

The results of the scoring taken at 3 different times in the life of the crop are given in Table<br />

4 below:<br />

Pre-em. <strong>MCPA</strong> 3ptsjacre<br />

Pre-em. <strong>MCPA</strong> 5ptsjacre<br />

Pre-em. Pesco 18-15 3ptsjacre<br />

Pre-em. Pesco 18-15 5ptsjacre<br />

Post-em. <strong>MCPA</strong> 3ptsjacre ..<br />

Post-em. <strong>MCPA</strong> 5ptsjacre ..<br />

Post-em. Pesco 18-15 3ptsjacre<br />

Post-em. Pesco 18-15 5pts/acre<br />

Hand Weeding<br />

No Weeding ..<br />

TABLE 4<br />

SCORE <strong>FOR</strong> CROP HEALTH<br />

The immediate effect of the herbicide on the<br />

development of the crop was evident soon<br />

after application, during the germination of<br />

the seed. The seedlings that emerged from<br />

the seed in the pre-emergence treatment plots<br />

were all unusually yellow and were considerably<br />

deformed. The extreme effect of the<br />

herbicides on the seedlings at th<strong>is</strong> stage <strong>is</strong><br />

evidently due to the fact that the spray fell<br />

more or less directly on the seed. Perhaps<br />

when the seed <strong>is</strong> dibbled or drilled at depth<br />

the adverse effect will not be so drastic.<br />

By the 4th of November, 12 days after pre-<br />

"mergence application, when the first scoring<br />

was done, the figures (table 4) indicate that<br />

the crop still showed a severe check in growth<br />

from the pre-emergence application. Recovery<br />

to normal growth was rather slow with<br />

all pre-emergence treatments, except with<br />

the lower level of <strong>MCPA</strong> at 3pts/acre. In th<strong>is</strong><br />

case recovery was fairly quick and by the 18th<br />

of November, 26 days after application, the<br />

crop had almost completely recovered.<br />

Growth was normal under all other treat .•<br />

ments except in the 'hand weeded' plots. In<br />

these plots growth and establ<strong>is</strong>hment<br />

deteriorated a bit after the first scoring. Th<strong>is</strong>


was apparently due to the periodic d<strong>is</strong>turbances<br />

in the plot at times of weeding, resulting<br />

from trampling and also possible mechanical<br />

damage to the crop during hoeing.<br />

Weed Control.- The relative effectiveness<br />

of different treatments on the control of weeds<br />

was also assessed by scoring at the same time<br />

as scoring was done for crop health. The<br />

following scale (Table 5), also a modification<br />

of one used by Green and Kaloger<strong>is</strong> (1963) in<br />

Arusha, was used.<br />

PeE <strong>MCPA</strong> 3 pts/ac 1<br />

PeE <strong>MCPA</strong> 5 pts/ac 2<br />

PeB Pesco 3 pts/ac 3<br />

PeE Pesco 5 pts/ac 4<br />

PoB <strong>MCPA</strong> 3 pts/ac 5<br />

PoE <strong>MCPA</strong> 5 pts/ac 6<br />

PoE Pesco 3 pts/ac 7<br />

PoE Pesco 5 pts/ac 8<br />

Hand Weeding 9<br />

No Weeding '10<br />

TABLE 6<br />

SCORES OF WEED DENSITY<br />

Weeding Control.-On the 4th of November,<br />

12 days after pre-emergence application<br />

weed growth in the treated plots were much<br />

less than in the untreated plots with the<br />

exception, of course, of the 'hand weeded'<br />

plots. On the 18th of November, 26 days<br />

after pre-emergence application the effect<br />

of the herbicide seemed to have waned<br />

.off a bit with the lower levels of pre-emergence<br />

application of either <strong>MCPA</strong> or<br />

Pesco 18-15, but control in the pre-emergence<br />

plots was still very much better than in<br />

the untreated plots, except the 'hand weeded'<br />

':p1ots'. At th<strong>is</strong> time the post-emergence<br />

application had not been given should be<br />

, oonsidered untreated.<br />

TABLE 5<br />

WEED DENSITY SCALE<br />

Score Reference<br />

5 No weeds<br />

4 Few weeds<br />

3 Moderate stand of weeds<br />

showing symptoms of suppression<br />

by herbicides<br />

2 Dense stand of healthy weeds<br />

1 Very dense stand of healthy<br />

weeds<br />

o Complete cover by weeds.<br />

Table 6 below gives the scores recorded on the<br />

three different dates for the various treatments.<br />

By the 11th of February, i.e. 92 days<br />

after pre-emgergence application, the effect<br />

of the herbicide applied pre-emergence<br />

seemed to have faded away completely.<br />

Th<strong>is</strong> was reflected in the predominance of<br />

weeds in these plots. The post-emergence<br />

application, which had been applied 41<br />

days before, showed a remarkably better<br />

control, compared with the pre~emergence<br />

application plots. .<br />

The life of a herbicide after its application<br />

in the field determines the duration of its<br />

effectiveness. Leaching and biological<br />

activities are the two main factors leading


to the d<strong>is</strong>appearance of herbicides in the<br />

field. Both <strong>MCPA</strong> and Pesco 18-15 are<br />

highly soluble in water and are likely to<br />

leach out of the top soil very quickly. Unde.r<br />

the flooded conditions of irrigated rice<br />

fields, leaching <strong>is</strong> likely to be even more<br />

intensive and the residual effect of these<br />

herbicides will not be. long. The residual<br />

effect of such herbicides in the soil will<br />

generally be related to their quantity of<br />

active ingredient per unit land surface.<br />

The evidence from th<strong>is</strong> study confirms<br />

th<strong>is</strong>. At. the higher dosage rate of 5ptsjacre<br />

the length of the weed control period was<br />

longer with the pre-emergence application<br />

of both <strong>MCPA</strong> and Pesco 18...:15than at<br />

the lower dosage rate of 3ptsjacre. For<br />

longer residual effects, perhaps larger dosage<br />

rates may be used if the crop tolerance <strong>is</strong><br />

high. A delayed application of the herbicide<br />

relative to the life of the crop also affords<br />

an opportunity of extending weed control<br />

into the latter part of the crops life. Th<strong>is</strong><br />

TABLE 7<br />

was attempted with the post-emergence<br />

application but the stage of application in<br />

relation to the weeds was perhaps so late<br />

that effective kit! was slow to achieve.<br />

The effect of the herbicide on the composition<br />

of the weed flora was assessed rather<br />

empirically and note was only made of the<br />

most striking species in the plots. The<br />

results of th<strong>is</strong> assessment cannot therefore,<br />

be very conclusive. lschaemum rugosum,<br />

a mono cot, was significant in all plots;<br />

Sphenoclea zeylanica was significant in all<br />

pre-emergence plots and also· in the unweeded<br />

plots; Cyperus difform<strong>is</strong> was particularly<br />

significant in the 'unweeded' plots.<br />

Yields.-The yields obtained for the<br />

different treatments were subjected to an<br />

analys<strong>is</strong> of variance. Th<strong>is</strong> showed significant<br />

differences due to treatments. Table 7<br />

below shows the mean yield of treatments<br />

arranged in ranked order for compar<strong>is</strong>on,<br />

using Duncan's multiple range test.<br />

EFFECTS OF TREATMENT ON <strong>THE</strong> YIELD OF MAIZE<br />

(a) Standard Error of the mean - ± 4.01<br />

(b) Shortest significantRange (Rp)<br />

p: (2) (3) (4) (5) (6) (7) (8) (9) (10)<br />

Rp 2.97 3.12 3.21 3.27 3.32 3.35 3.37 3.39 4.41<br />

(c) Ranked Means of Treatment Yields<br />

Pre-em Pre-em Pre-em Pre-em No Post-em Post-em Clean Post-em Post-em<br />

Treatment: <strong>MCPA</strong> Pesco Pesco <strong>MCPA</strong> Weed- Pesco <strong>MCPA</strong> Weed- Pesco <strong>MCPA</strong><br />

18-15 18-15 ing ing<br />

5pt/ac 5pt/ac 3pt/ac 3pt/ac 5pt/ac 3pt/ac 3pt/ac 5pt/ac<br />

Yield/plot 1.8 6.8 7.5 8.7 11.7 21.3 23.2 25.7 27.2 28.2<br />

lb./acre 101.5 387.5 430.5 498.0 669.9 1224.3 1330.0 1473.5 1559.6 1617.0<br />

Th<strong>is</strong> compar<strong>is</strong>on showed clearly significant<br />

differences in yields between the pre-emergence<br />

application on the one side and the<br />

post-emergence application on the other<br />

side. Yield from the unweeded treatment<br />

was, however, not significantly different<br />

from those with post-emergence application<br />

of Pesco 18-15 at 5ptsjacre and those with<br />

post-emergence application of <strong>MCPA</strong> at<br />

3ptsjacre.<br />

Two principal factors accounted for yield<br />

differences of the different treatments. These<br />

are (a) the relative crop tolerance to the<br />

herbicide and (b) the degree of effective weed<br />

control. The effect of weed control without<br />

any interference from herbicide can be<br />

obtained by direct compar<strong>is</strong>on between the<br />

unweeded and the clean weeded treatments.<br />

These showed a remarkably significant<br />

difference due to competition from weeds.<br />

The highest mean yields were obtained<br />

from the post-emergence application of<br />

Pesco 18-15, at 3ptsjacre, and <strong>MCPA</strong>, at<br />

5ptsjacre, although these were not significantly<br />

better than the mean yield of the<br />

clean weeded treatment.<br />

It was quite evident that yields were<br />

significantly affected by crop tolerance. In<br />

th<strong>is</strong> respect time of application was a more<br />

significant factor. The most significant


evidence for th<strong>is</strong> came from <strong>MCPA</strong>. While<br />

<strong>MCPA</strong> at 5ptsjacre produced the highest<br />

yield among all treatments when applied<br />

post-emergence, the same dosage produced<br />

the least at pre-emergence. Within the preemergence<br />

application there was also an<br />

indication that the crop's tolerance of both<br />

herbicides was lower at the higher dosage<br />

rate of 5ptsjacre than at 3pts/acre. With<br />

post-emergence application there was<br />

evidence of increasing toxicity with Pesco<br />

18-15 at higher dosage (5ptsjacre) than at the<br />

lower dosage rate (3ptsjacre) in terms of<br />

yields. <strong>MCPA</strong>, however, showed greater<br />

efficiency with increasing dosage, and may be<br />

tried at higher rates for more effective weed<br />

control.<br />

References<br />

LE CLERG,B.L., LEONARD,W.H. & CLARK,<br />

A. G. (1962) Field Plot Technique, Burgess<br />

Publ<strong>is</strong>hing Company, Minnesota.<br />

DUNCAN, D.B. (1955) Multiple F-tests.<br />

Biometrics, 11, 1-42.<br />

GREEN, D.H. & KALoGERIS,B (1963) A<br />

preliminary Herbicide screening trial on<br />

Ground. M<strong>is</strong>cellaneous Report No. 418.<br />

Tropical Pesticides Research Institute,<br />

Arusha.

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