Genomics - VWR International

Genomics 

Eppendorf Mastercycler® 

Fast qPCR with realplex 4 

Proteomics 

See page 3 

Pall AcroPrep Advanced 

Filter Plates for Ultrafiltration 

Microbiology 

AES CHEMUNEX 

Masterclave ® Series 

Cell Biology 

Lonza StellARay 

qPCR Arrays 

See page 23 

See page 27 

See page 33 

Featured in this issue: 

See Great Promotions Inside! 

MAGAZINE 

Volume 12 Issue 25 

Impacting 

DNA Research

Contents 

Life Science Magazine Volume 12 • Issue 25 

2-22 Genomics 

2-4 Reproducible High-Throughput Probe-Based qPCR 

5 illustra triplePrep Kit 

5 E.Z.N.A. HP Total RNA Kits 

5 PCR taq Polymerase 

6-7 Hi-Res Melting ® for Mutation Scanning Using LCGreen ® Plus 

8-10 Innovative Thermal Energy Recovery System (TERS ® ): 

A Novel Mechanism for Controlling Thermal Cycler Blocks 

11-12 Detection and Quantification of MicroRNAs in Human Tissues 

13 NIST-Candidate RNA Control Products 

13 PCR Workstation with Stainless Steel Work Surface 

14-15 Demonstration of FlashGel Recovery with Sheared DNA 

16 EvaGreen ® qPCR Dye 

16 Kingfisher ® Nucleic Acid Purification Kits 

17 illustra GFX PCR DNA and Gel Band Purification Kits 

17 PyroScript ® RT-PCR 2X Master Mix 

17 Gel/PCR DNA Fragments Kits 

18 Gel Imagers 

18 Green PCR Reagents 

18 UV, Dual UV, and Variable Intensity Transilluminators 

19 Multiskan ® GO UV/Vis Spectrophotometer 

19 Blocking Solutions, Buffers, and Substrates 

20-21 Mouse Genotyping In Less than One Hour 

22 Hazard Reducing RNA Kits 

22 RNA Purification Dyes and Solutions 

22 ExiSpin Combination Vortex/Centrifuge 

23-25 Proteomics 

23 AcroPrep Advance Filter Plates for Ultrafiltration 

23 Enzyme Immunoassay Kits and Peptides 

24 Spin-X ® UF Concentrators 

24 SuperSignal ® Western Blot Enhancer 

25 Kodak BioMax ® Film 

25 pH Test Strips and DoubleZone Indicator Papers 

26-27 Microbiology 

26 UV Crosslinker 

26 UV Hand Lamps 

26 PolySeed ® for BOD5 Analysis 

27 Masterclave ® Series Automated Culture Media Preparators 

27 Culture Media and Media Bases 

28,33 Cell Biology 

28 Antibodies and Conjugates 

28 cellgro ® Molecular Biology Grade Water 

28 Electroporation Systems 

33 StellARray qPCR Arrays 

33 jetPRIME DNA and siRNA Transfection Reagents 

29-32 Pull-Out Supplement 

29-30 VWR Life Science Portfolio 

31-32 VWR Life Science Promotions 

2 Life Science Magazine vwr.com 800.932.5000

INTRODUCTION 

In contrast to sequence-nonspecific intercalating dyes that 

bind indiscriminately to double-stranded DNA (e.g. SYBR ® 

Green I), sequence-specific fluorogenic probe chemistries 

only quantify the accumulation of specific PCR product. 

This improved specificity eliminates the need for post-PCR 

melt curve analysis and permits real-time multiplexing. 

Common probe chemistries include TaqMan ® , FRET 

probes, scorpion probes, and molecular beacons. Standard 

probe-based qPCR cycling protocols take between 1 hour 

10 minutes and 1 hour 30 minutes, depending on the 

ramp rate and data acquisition times of the specific qPCR 

instrument used (Figure 1). Faster cycling protocols 

(~30 minutes) that do not compromise reaction efficiency 

typically require instrumentation with increased thermal 

ramp rates, uniform heating across the block, and specially 

formulated reagents. 

The Eppendorf realplex 4 S real-time PCR system features 

a super-fast, Peltier-controlled thermoblock that heats 

up at 6°C/sec. and cools down at 4°C/sec., which allows 

completion of a 40-cycle qPCR reaction in less than 

1 hour. These Peltier units also ensure accurate 

temperature control as well as high block homogeneity, 


Reproducible High-Throughput Probe-Based qPCR 

The Eppendorf Mastercycler ® ep realplex 4 S real-time PCR instrument provides an industry-leading 

solution for high-performance, fast qPCR without compromising reaction efficiency and reproducibility. 

Figure 1: Total qPCR times for either a standard reaction 

protocol (10 minutes at 95°C activation, 40 cycles of 15 seconds 

at 95°C, and 60 seconds at 60°C) or a fast protocol (3 minutes at 

95°C activation, 40 cycles of 3 seconds at 95°C, and 20 seconds 

at 60°C). Reactions were performed on a slow ramp rate qPCR 

thermocycler (avg. ramp rate 1°C/sec.) and the fast Eppendorf 

realplex 4 S real-time PCR system (6°C/sec. heating and 

4°C/sec. cooling). 

which are essential to high-quality qPCR reactions. The 

optical module comprises an array of 96 LEDs that are 

preselected and equalized so that they excite all wells 

uniformly. This setup also eliminates the requirement for 

ROX reference dye that is used to normalize fluorescent 

signals by many conventional qPCR instruments. The 

realplex 4 also utilizes the Photo Multiplier Tube (PMT) 

for signal detection, which is the most sensitive and 

affordable detection technique currently available. In 

addition, this four-channel system provides full multiplex 

flexibility through emission filters corresponding to 

520/550/580/605nm. 

METHODS 

To demonstrate the ability to perform fast qPCR cycling 

without compromising performance, a set of five 10-fold 

serial dilutions of human genomic DNA (hgDNA) was 

amplified using either a standard or fast cycling protocol. 

Amplification of the 74bp hApoB100 amplicon was 

performed in full-skirted Eppendorf twin.tec ® plates 

with optical clear heat-sealing film using the Eppendorf 

realplex 4 S real-time PCR system. 

The resulting log amplification plots and standard curves 

for reactions performed using the standard protocol 

(Figure 2) and fast protocol (Figure 3) indicate that 

both the CT and efficiency of the qPCR reactions have 

not been compromised as a result of using fast cycling 

protocols relative to standard cycling protocols (for a 

description of each protocol see Figure 1). The fast 

protocol results in a time saving 

of 43 minutes relative 

to the slow protocol 

when performed 

on the Eppendorf 

realplex 4 S real-time 

PCR system. 

3


Fluorescence (norm) 

Ct [Cycle] 

35 

30 

25 

20 

0.01 0.1 1.0 10 100 1000 

Amount[ng] 

Slope: -3.320 

Efficiency: 100% 

R2 400 

: 0.999 

0 10 20 

Figure 2: Standard cycling protocol (run time = 79 minutes). Data showing 

the log amplification plot across a set of five 10-fold serial dilutions of hgDNA 

ranging from 200ng to 20pg per reaction, using a standard 2-step cycling 

protocol (10 minutes at 95°C activation, 40 cycles of 15 seconds at 95°C, and 

one minute at 60°C). 20µL reactions were performed using KAPA PROBE 

FAST qPCR Master Mix on the Eppendorf realplex 4 S real-time PCR system. 

The data represents 7 replicates for each DNA dilution. The hApoB100 

assay targets a 74bp amplicon. 


40,000 

4,000 

40,000 

4,000 

Ct [Cycle] 

35 

30 

25 

20 

0.01 0.1 1.0 10 100 1000 

Amount[ng] 

Cycles 

Slope: -3.305 

Efficiency: 101% 

R2 400 

: 0.999 

0 10 20 

Cycles 

Figure 3: Fast cycling protocol (run time = 36 minutes). Data showing the 

log amplification plot across a set of five 10-fold serial dilutions of hgDNA 

ranging from 200ng to 20pg per reaction, using a fast 2-step cycling 

protocol (3 minutes at 95°C activation, 40 cycles of 3 seconds at 95°C, and 

20 seconds at 60°C). 20µL reactions were performed using KAPA PROBE 

FAST qPCR Master Mix on the Eppendorf realplex 4 S real-time PCR system. 

The data represents 7 replicates for each DNA dilution. The hApoB100 


Reduced running costs, particularly in combination with 

increased throughput, are always desirable. To illustrate the 

ability to use reduced reagent volumes with comparable 

results, three different reaction volumes (5, 10, and 20µL) 

were compared using the fast protocol (Figure 4). All three 

reaction volumes resulted in highly consistent amplification 

across a set of five 10-fold serial dilutions of hgDNA when 

cycled using the fast cycling protocol. 

Reactions were performed using KAPA PROBE FAST qPCR 

MasterMix, specially formulated for high-speed assays. In 

conjunction with the benefit of reduced evaporation during 

qPCR by using the Eppendorf realplex 4 S real-time PCR 

system, comparable results were obtained using low-volume 

qPCR reactions. 

4 Life Science Magazine vwr.com 800.932.5000 

30 40 

30 40 


25,000 

2,500 

20 µL 

10 µL 

5 µL 

250 

0 10 20 

Cycles 

30 40 

Figure 4: Comparison of different qPCR reaction volumes. Similar results 

were obtained when either 5, 10, or 20µL reaction volumes were used 

in the hApoB100 assay across a set of five 10-fold serial dilutions of hgDNA 

ranging from 200ng to 20pg per reaction, using a fast 2-step protocol. 

Reactions were performed using KAPA PROBE FAST qPCR Master Mix on 

the Eppendorf realplex 4 S real-time PCR system. The data represents a 

single reaction at each reaction volume and DNA dilution. The hApoB100 


CONCLUSION 

High performance, fast qPCR using probe-based chemistries 

requires a real-time instrument capable of extremely fast ramp 

rates with excellent uniformity across all samples, and a qPCR 

reagent formulated for high-speed and sensitivity. The Eppendorf 

realplex 4 S real-time PCR system utilizing KAPA PROBE FAST qPCR 

Kits provides high-performance, fast qPCR without compromising 

reaction efficiency and reproducibility. In addition, the ability 

to perform low-volume qPCR can reduce running costs while 

maintaining high quality results. 

REFERENCE 

1. Lawyer, F.C. et al., High-Level Expression, Purification and Enzymatic Characterization of 

Full-Length Thermus Aquaticus DNA Polymerase, and a Truncated Form Deficient in 5' to 3' 

Exonuclease Activity. PCR Methods Appl. 2, 275-287 (1993). 

Realplex 4 Systems Cat. No. Price 

2 System (Includes Laptop w/ 97060-540 Ea./ 27,950.00 

Aluminum Block & Two Emission Filters) 

2S System (Includes Silver Block 97060-544 Ea./ 28,750.00 

& Two Emission Filters) 

4 System (Includes Aluminum Block 97060-548 Ea./ 33,350.00 

& Four Emission Filters) 

4S System (Includes Silver Block 97060-552 Ea./ 34,150.00 

& Four Emission Filters) 

Accessories 

twin.tec PCR plate, 96-well Skirted, 47744-116 Cs. 25/ 108.58 

Clear 

Heat Sealing Film 89167-284 Pk. 100/ 210.15 

Heat Sealer 62111-428 Ea./ 2,185.60

illustra 

Purify. Amplify. Simplify. 

triplePrep 

Kit 

Purify DNA, RNA, and Protein from the 

Exact Same Sample with a Single Kit! 

The illustra triplePrep Kit is designed for rapid, 

simultaneous extraction and isolation of high 

quality genomic DNA (gDNA), total RNA, and 

total denatured proteins from animal tissues 

and mammalian cells. High yields of DNA, RNA, 

and proteins can be extracted in less than one hour using a flexible, 

easy-to-follow workflow allowing researchers to directly correlate data 

generated from the same sample. 

The isolated gDNA, total RNA, and total denatured proteins are suitable 

for genomic and proteomic applications such as PCR, restriction enzyme 

digestion, sequencing, array CGH, RT-PCR, gene expression microarray, 

SDS-PAGE, Western blotting, 2-D DIGE, and LCMS. 

• Conserves Precious Sample – Purifies all 

three analytes at once from the same, undivided 

sample using a single kit/protocol 

• Minimizes Sample Prep Variability – 

Associated with purifying analytes individually 

• Fast – Get from sample to DNA/RNA/protein 

in less than one hour – up to 70% time 

savings compared to using individual 

analyte purification kits/protocols 

Description Cat. No. Pack of 50 Preps 

triplePrep Kit 95056-354 464.00 

E.Z.N.A. HP Total RNA Kits 

Remove Genomic DNA Without Enzymes 

Provides a rapid and easy method for RNA isolation from a small amount 

of cultured eukaryotic cells or tissues. This kit allows single or simultaneous 

processing of multiple samples in less than 30 minutes. Normally, 1 x 10 7 

eukaryotic cells, or 25-30mg tissue, can be used in a single experiment. 

There is no need for phenol/chloroform extractions, and time-consuming steps 

such as CsCl gradient ultracentrifugation, and precipitation with 

isopropanol or LiCl, are eliminated. RNA purified using the E.Z.N.A. HP Total 

RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern 

blotting, poly A+ RNA (mRNA) purification, nuclease protection, and 

in vitro translation. 

Description Preps Cat. No. Price 

HP Total RNA Kit 50 101414-850 175.11 

HP Total RNA Kit 200 101414-852 582.42 

Viral RNA Miniprep Kit 50 101319-254 132.16 

Viral RNA Miniprep Kit 200 101319-256 463.71 

A Highly Thermostable 

DNA Polymerase 


Isolated from the thermophile Thermus aquaticus, Taq Polymerase efficiently 

catalyzes the 5' to 3' synthesis of DNA. The enzyme has no detectable 3' to 5' 

proofreading exonuclease activity, and possesses low 5' to 3' exonuclease 

activity. The error rate of G-Biosciences’ Taq Polymerase is low at only 

~2.2 x 10 -5 nucleotide -1 cycle -1 . Our Taq Polymerase is highly sensitive 

and is able to amplify DNA up to 6Kb from only a single adenine 3' overhang. 

The product is supplied with 10X PCR buffer and magnesium chloride. 

• 5' to 3' polymerase activity 

• Error rate ~2.2 x 10 -5 nucleotide -1 cycle -1 

• No detectable 3' to 5' proofreading 

exonuclease activity 

PCR Taq Polymerase, Size Cat. No. Price 

100U 95057-680 42.84 

250U 95057-682 193.80 

500U 95057-684 166.26 

1,000U 95057-686 331.50 

Life Science Magazine vwr.com 800.932.5000 5


Hi-Res Melting ® 

For Mutation 

Scanning Using LCGreen ® 

Plus 

INTRODUCTION 

Idaho Technology began offering Hi-Res Melting (also known as 

HRM or HRMA) systems and LCGreen chemistry in 2003. Together 

with the University of Utah, specifically Carl Wittwer’s group, we 

invented the technology. Screening amplified DNA for sequence 

variation, also known as “mutation scanning,” is an important 

tool for genetic research and clinical applications. Mutation 

scanning techniques detect the presence of sequence variation 

in a fragment of amplified DNA. Conventional scanning techniques 

are not homogenous and require a separation step to identify 

heteroduplexes. The LightScanner ® system is unique in that it 

allows homogenous mutation scanning in standard microtiter 

format using a dsDNA binding dye, LCGreen Plus, and high 

resolution melting. 

HI-RES MELTING OVERVIEW 

Hi-Res Melting of nucleic acid depends on the ability to 

collect high-density information of fluorescence as a function 

of temperature in a mixture that contains a fluorescent doublestrand 

DNA binding dye and PCR product. Mutations in PCR 

products are detected by changes in the shape of the melting 

curve compared to a reference sample. 


LCGREEN 

The LightScanner system utilizes the fluorescence of the highest 

class of dsDNA binding dye, LCGreen Plus, to identify sequence 

variations without the need for dye-labeled probes. LCGreen Plus 

dye is specifically designed for Hi-Res Melting curve analysis to 

detect DNA sequence variants. It is unique in its ability to detect 

the presence of heteroduplexes formed during PCR. 

LCGreen Plus is added to the reaction prior to PCR. Supplied at a 

10X concentration, simply add one volume of the dye solution for 

every nine volumes of the PCR mixture during PCR set up. For 

highest reproducibility in many settings, coupled with easiest use, 

a complete Hi-Res Melting master mix is available from Idaho 

Technology, Inc. (see product info, below). The LightScanner 

Master Mix is an optimized PCR master mix, that includes LCGreen 

Plus, and was developed specifically for Hi-Res Melting applications. 

THE LIGHTSCANNER SYSTEM 

The LightScanner system requires no post-PCR addition of reagents 

or the need for expensive and time-consuming separation. LCGreen 

Plus is included in the amplification reaction. The Hi-Res Melting 

profile reveals heterozygous single-base changes in five minutes 

with a sensitivity and specificity superior to non-homogenous 

techniques, such as DHPLC or TGCE. In addition to identifying 

anonymous heterozygous variants, the system enables identification 

of specific mutations, in such cases scanning and genotyping can

often be combined into one simple melting analysis. The post-PCR 

product remains intact, thus enabling down stream analysis such as 

sequencing. New work has shown that somatic mutation sensitivity is 

typically higher than Sanger sequencing, with 5-20% mutation levels 

readily detectable on the LightScanner in scanning mode using no 

other special techniques. 

ADDITIONAL INFORMATION 

A license is required from Idaho Technology for the manufacturing 

and commercial use of LCGreen dyes and/or High-Resolution Melting 

(High-Res Melting ® ) technology. For a licensing package, please 

contact us directly or send an email to it@idahotech.com. 

The purchase of the LightScanner instrument includes a limited, 

nontransferable instrument license under specific claims of one 

or more U.S. patents as listed on Idaho Technology, Inc.’s Web Site 

(http://www.idahotech.com/LegalNotices/), which is owned by the 

University of Utah Research Foundation and/or Idaho Technology, Inc. 

Any kits sold with this product and/or discussed herein (i) may be 

covered by one or more of the U.S. patents, as listed on the Web Site 

for the product and (ii) include a limited, nontransferable license to 

use the enclosed amount(s) in such kits according to the specified 

protocols. Purchase of the LightScanner Master Mix does not convey 

any PCR license. The LightScanner Master Mix is licensed under 

U.S. Patent Nos. 5,338,671 and 5,587,287. The purchase of the 

LightScanner Master Mix and/or the LightScanner instrument includes 

a limited, non-transferable license for all fields other than human or 

veterinary in vitro diagnostics under U.S. Patent No. 5,871,908, owned 

by Evotec Biosystems GmbH and licensed to Roche Diagnostics 

GmbH, to use only the enclosed amount of product according to the 

specified protocols. No right is conveyed, expressly, by implication, 

or by estoppel, to use any instrument or system under any claim 

of U.S. Patent No. 5,871,908, other than for the amount of 

product contained herein. 

LCGreen ® , Hi-Res Melting ® and LightScanner ® are registered trademarks of 

Idaho Technology, Inc. SYBR ® is a registered trademark of Molecular Probes, Inc. 


REFERENCES 

1. Gonzalez-Bosquet J, Calcei J, Wei JS, Garcia-Closas M, Sherman ME, et al. 2011 Detection 

of Somatic Mutations by High-Resolution DNA Melt-ing (HRM) Analysis in Multiple 

Cancers. (2011). PLoS ONE 6(1): e14522. 

2. De Koeyer D, Douglass K, Murphy A, Whitney S, Nolan L, Song Y, De Jong W (2010). 

Application of High-Resolution DNA Melting for Genotyping and Variant Scanning of Diploid 

and Autotetraploid Potato. Mol Breeding. 25, 67-90. 

3. Rolf H.A.M. Vossen, Emmelien Aten, Anja Roos, Johan T. den Dunnen. (2009). 

High-Resolution Melting Analysis (HRMA) - More Than Just Sequence Variant Screening 

Human Mut. 30, 860-866. 

4. Parant JM, George SA, Pryor R, Wittwer CT, Yost HJ. (2009). A Rapid and Efficient Method 

of Genotyping Zebrafish Mutants. Dev Dyn. 238,3168-74. 

5. van der Stoep N, van Paridon CDM, Janssens T, Krenkova P, Stambergova A, Macek M, 

Matthijs G, Bakker E. (2009). Diagnostic Guidelines for High-Resolution Melting Curve 

(HRM) Analysis: An Interlaboratory Validation of BRCA1 Mutation Scanning Using the 

96-well LightScanner. Human Mut. 30, 899-909. 

6. Aten E, White SJ, Kalf ME, Vossen RH, Thygesen HH, Ruivenkamp CA, Kriek M, Breuning 

MH, den Dunnen JT.(2008). Methods to Detect CNVs in the Human Genome. Cytogenet 

Genome Res. 123, 313-21. 

7. Audrezet MP, Dabricot A, Le Marechal C, Ferec C. (2008). Validation of High-Resolution 

DNA Melting Analysis for Mutation Scanning of the Cystic Fibrosis Transmembrane 

Conductance Regulator (CFTR) Gene. J Mol Diagn. 10, 424-34. 

8. C. Voegele, S.V. Tavtigian, D. de Silva, Cuber Thomas, F. Le Calvez-Kelm. (2007). 

A Laboratory Information Management System (LIMS) for a High Throughput Genetic 

Platform Aimed at Candidate Gene Mutation Screening. Bioinformatics. 23, 2504-2506. 

Description Cat. No. Price 

LightScanner Instrument, 96-well 89168-794 38,000.00 

LightScanner Instrument, 384-well 89168-796 40,000.00 

LightScanner 32 qPCR Plus Hi-Res 

Melting Apparatus 

89168-798 29,500.00 

LCGreen Plus Gene Scanning 

Reagents Kit, 1,000 rxn 

89168-818 115.00 

LCGreen Plus Gene Scanning 

Reagents Kit, 10,000 rxn 

89168-820 900.00 

LightScanner Master Mix, 100 rxn 89168-802 120.00 

LightScanner Master Mix, 500 rxn 89168-804 375.00 



Innovative Thermal Energy Recovery System (TERS ® 

): 

A Novel Mechanism for Controlling Thermal Cycler Blocks 

INTRODUCTION 

Thermo electric coolers, also known as Peltiers, have long been 

used as the mechanism by which instruments such as thermal 

cyclers control the temperature of the blocks holding the reaction 

vessels. The precise way in which they can be controlled is essential 

for specific processes such as PCR. The current trend for rapid 

PCR cycling requires even faster heat exchange processes to occur 

without compromising the uniformity specification of the block. 

In this article we introduce the TERS system of the Techne TC-PLUS 

thermal cycler. This technology uses a novel energy saving 

mechanism to improve the ramp rates of a standard aluminum 

thermal cycler block (Figure 1). 

Figure 1: The TC-PLUS thermal cycler with three satellite units. The 

TC-PLUS features a color touch screen interface and unique space-saving 

stackable design. Up to seven satellite units can be controlled from a 

TC-PLUS, or alternatively can be controlled using PC software. 


RAPID CYCLE PCR 

In the modern laboratory, speed is often of the essence, and any 

process capable of saving valuable research time is an advantage. 

An increasingly popular technique in this direction, with many 

recent new products on the market, is that of fast PCR. Rapid cycle 

PCR was developed about 20 years ago with the invention of hot 

air thermal cyclers 1 . Samples were placed in thin glass capillary 

tubes and subjected to cycles of hot and cold air. The rapid heat 

transfer meant that PCR products could be amplified in as little 

as 15 minutes. More recently, advances in block technology have 

seen the introduction of conventional thermal cyclers capable of 

temperature ramp rates of up to 15ºC/sec. 

RAMP RATES & FAST PCR PROTOCOLS 

The factors that determine the length of a PCR run include the 

number of steps and cycles (including any initial denaturation/ 

enzyme activation and final extension steps), the hold times of 

these steps, and the ramp rates between them. Ramp rates are 

an important part of the PCR as primer annealing and product 

extension will occur during these phases. Simply accelerating the 

ramp rate for heating and cooling of a PCR run without changing 

other program parameters can greatly reduce the total run time 

without compromising on sensitivity (Figure 2). 

Figure 2: A 231bp fragment of the pBR322 plasmid vector was amplified 

in parallel in both TC-PLUS and TC-512 thermal cyclers, which have maximum 

heating ramp rates of 5.0ºC/sec. and 3.6ºC/sec. respectively. The numbers 

shown are the copies of template added to each 20µL reaction. NTC is a no 

template control. For this particular reaction, the results demonstrated no 

appreciable differences in yield or sensitivity between the two thermal 

cyclers. The program took approximately 1 hour to complete when using 

the TC-PLUS compared to 1 hour 30 minutes in the TC-512; saving 

approximately 30 minutes on the run time entirely due to the increased 

ramp rate of the TC-PLUS.

Fast PCR protocols, on the other hand, rely on greatly reducing 

the hold times for denaturation and elongation steps. Without 

proper optimization and the use of specifically formulated reagents 

that contain specialized enzymes and stabilizers, the reactions can 

show a loss of sensitivity and increased variability 2 . 

Traditionally, block thermal cyclers have used expensive gold-plated 

silver blocks in order to achieve the required rapid ramping speeds 

demanded by users. Silver has the highest thermal conductivity of 

any metal, but has the tendency to tarnish in air. Gold also has a 

high thermal conductivity, but is resistant to oxidative corrosion. 

Traditional blocks are made of aluminum, which has only about 

half the thermal conductivity of silver and is therefore more limited 

in ramp rate. The TERS system developed for the Techne TC-PLUS 

thermal cycler uses a unique means of controlling the thermal 

energy dissipated by the heat sink in order to offer an improvement 

in ramp rates compared to conventional aluminum blocks. The 

result is a ramp rate comparable to silver blocks but with better 

block uniformity and lower cost. 

THERMO ELECTRIC COOLERS (TECS) 

TECs, or Peltiers, are semiconductor devices that transfer energy 

from one face to the other when a DC current is passed through 

them; this phenomenon is known as the Peltier effect. The direction 

of energy transfer is reversed when the applied current is reversed. 

The face of the device that energy is pumped from is known as the 

cold side and the face that energy is transferred to is known as the 

hot side (Figure 3). 

Any energy dissipated by the TEC is pumped to the hot side of the 

device. The amount of energy the TEC can pump from the cold to 

hot side, Qc, varies with ΔT; when ΔT ≤0 then the pumped energy 

is at its maximum, Qcmax. Qc reduces to zero when ΔTmax is 

reached. ΔTmax depends on the quality and type of the TEC. 

Thermal cyclers use TECs to control the temperature of the samples 

in the well plate of the thermal block. The TEC’s ability to pump 

energy allows the samples to be heated and cooled rapidly. A heat 

sink is used to dissipate energy and keep the temperature on the 

side of the TEC opposite the samples as close to ambient as 

possible. Typically the heat sink is force-air cooled using fans. 


Figure 3: Definition of some terms relating to operation of a TEC. Qc is the 

amount of thermal energy pumped from attached objects e.g. thermal cycler 

well plate. ΔT is the difference in temperature between the hot side and the 

cold side of the TEC. 

THERMAL ENERGY RECOVERY SYSTEM (TERS) 

It is desirable to maximize the heating and cooling rate in a 

thermal cycler in order to minimize the total time needed for an 

experiment. If the temperature profile required is known, or can 

be predicted, and it is possible to control the amount of energy 

dissipated by the heat sink, the temperature of the heat sink can 

be controlled to offer improved ramp rates. In a system using 

forced air to cool the heat sink, fan speed can be altered to 

change the heat dissipation. 

The improvement is gained by reducing the ΔT across the TEC 

during temperature transitions of the well plate. As the well plate 

is cooled, the energy from the well plate and dissipated electrical 

energy is pumped to the heat sink, hence its temperature rises. 

By dissipating very little of this energy from the heat sink, the 

temperature of the heat sink can be maintained at a desired level. 

The heat sink temperature can be chosen to minimize the ΔT 

across the TECs when the well plate temperature is next altered. 

The reduction in ΔT results in an increased amount of energy 

pumped and conducted to the well plate, which in turn gives 

a faster ramp rate (Figure 4 on Page 10). 

In this way, the heat sink is used as an energy store as well 

as a dissipater. The same improvement in ramp rates can be 

achieved wherever a TEC is installed and required temperature 

changes are known or can be predicted. 



T 

Temp 1 

Ambient 

COOLING PHASE 

Temp 2 

The TEC has to switch from 

cooling to heating at this point 

HEATING PHASE 

Temp 3 

Time 

Time 

Time 

Top of TEC 

(well plate side) 

The TEC is cooling until this point so the T 

becomes negative when the well plate is heated 

Bottom of TEC with 

variable heat sink 

dissipation (TERS) 

Bottom of TEC with 

maximum heat 

sink dissipation 

TEC with maximum 

heat sink dissipation 

TEC with variable 

heat sink dissipation 

(TERS) 

Figure 4: A sketch illustrating how the varying heat sink energy dissipation 

can reduce ΔT and increase ramp rates. After the cooling phase, the fans 

are slowed or stopped to hold the heat sink at an elevated temperature. 

During the heating phase, the ΔT of the TEC with variable heat sink 

dissipation is much lower than that with maximum heat sink dissipation, 

resulting in a higher Qc and a faster ramping rate. 

ADDITIONAL ADVANTAGES OF TERS 

Due to the fact that a portion of the heat energy is conserved in the 

heat sink and not dissipated by the fans, and also because the fans 

are turned off for short periods of time during each cycle, it can 

be surmised that this would amount to significant energy savings 

in the running of the thermal cycler. This was tested by running 

the TC-PLUS through typical 3-step and 2-step PCR protocols 

and measuring the energy consumed per run (Figure 5). It was 

found that for a typical 3-step program, almost 15% less energy 

was required with variable heat dissipation activated and around 

9% less for a 2-step protocol. This would amount to considerable 

cost savings over the life of the thermal cycler, especially during 

heavy use. 

CONCLUSION 

The TERS system offers a number of advantages for the TC-PLUS 

user, including time-saving rapid ramp rates, lower initial cost of 

the aluminum block compared to silver blocks required for other 

similar rapid ramping systems, and overall energy savings during a 

run. In addition, optimizing reactions with specialized reagents for 

fast PCR protocols also leads to shorter run times, increased 

throughput, and lower energy costs. 


Energy consumed (kWh) 

0.60 

0.50 

0.40 

0.30 

0.20 

0.10 

0.00 

Figure 5: Energy consumed by the TC-PLUS during a thermal cycling 

protocol. Typical 3-step and 2-step PCR programs were run on the same 

unit with and without the TERS function activated. Each program was run 

three times and the energy consumption measured using a plug-in power 

and energy monitor. The average energy consumptions for the three runs 

are shown. 

REFERENCES 

1. Wittwer C T & Garling D J. Rapid Cycle DNA Amplification: Time and Temperature 

Optimization. Biotechniques 1991; 10(1): 76-83. 

2. Hilscher, C, Vahrson, W & Dittmer, D P. Faster Quantitative Real-Time PCR Protocols 

May Lose Sensitivity and Show Increased Variability. Nucl. Acids Res. 2005; 33(21): e182. 

89130-738 

Description Size Cat. No. Price 

TC-PLUS, Gradient TERS 96x0.2mL 89130-738 8,735.00 

TC-PLUS, Gradient TERS 60x0.5mL 89130-740 8,735.00 

TC-PLUS, Gradient TERS 384-well 89130-742 8,735.00 

TC-PLUS SAT, Gradient TERS 96x0.2mL 89130-744 6,445.00 

TC-PLUS SAT, Gradient TERS 60x0.5mL 89130-746 6,445.00 

TC-PLUS SAT, Gradient TERS 384-well 89130-748 6,445.00

Detection and Quantification of MicroRNAs 

in Human Tissues 

MicroRNAs are small, non-coding RNAs implicated in gene 

regulation and many important diseases and biological pathways 

including neuronal function, cancer, and heart disease. 

Identification of present microRNA sequences and quantification 

of the mature microRNAs – also known as miRNAs or miRs – are 

important steps in understanding the roles they play and in 

validating them as biomarkers or therapeutic targets. 

MicroRNA is: 

Key to many important biological pathways and diseases 

Present in all eukaryotic tissues 

Present in different tissues in different configurations and in 

varying quantities 

Believed to be very rare 

ADVANCES IN MICRORNA PROFILING 

Many microRNAs have been sequenced and deposited in miRbase, 

a public microRNA database (www.mirbase.org). Profiling of 

microRNAs in various tissues has been conducted using platforms 

including microarrays, sequencing, and quantitative reversetranscriptase 

PCR (RT-qPCR). Quanta Biosciences has successfully 

profiled microRNAs using RT-qPCR and SYBR Green I detection 

on commonly available equipment, and with a broad range 

of starting material. 

Quanta Biosciences has developed a microRNA quantification 

system that includes first-strand synthesis and SYBR Green qPCR 

(Figure 1). The qScript microRNA Quantification System may be 

used with total RNA or preparations pre-enriched for microRNAs. 

Proportional representation of the microRNA sequences is achieved 

in the first-strand product over a broad range of template input 

as shown in the 

example of hsa-miR-1 

(Figure 2). This is 

critical because 

microRNA abundance 

can vary greatly 

among different 

samples or tissues, 

and some tissues or 

cell types are rare 

or precious. 

Poly(A) Tailing 

cDNA Synthesis 

qRT-PCR with 

SYBR Green 

SuperMix 

Relative Fluorescence Units 

1,600 

1,400 

1,200 

1,000 

800 

600 

400 

200 

0 

5’ 

MicroRNA 

3’ 

Poly(A) Polymerase 

dNTPs 

Reverse 

Transcriptase 

microRNA Assay Primer 


ATP 

AAAAAAAAAAAAAAA 


AA 

Oligo-dT Adapter Primer 


Figure 1 

miR-1 in Heart 

Figure 2 

Universal PCR 

Primer 

0 5 10 15 20 25 30 35 40 

Number of PCR Cycles 

– –– 10.0ng 

– –– 1.0ng 

– –– 0.1ng 

– –– 10.0pg 

– –– 1.0pg 

– –– 0.1pg 



Relative Abundance 

Relative Abundance of MicroRNAs in Human Tissues 

5,000 

4,000 

3,000 

2,000 

1,000 

0 

4,309 

miR-1 

10 1 

Heart Brain Liver 

miR-1 

1,600 

1,200 

PROFILE WIDE RANGE OF MICRORNAS FROM ANY SAMPLE 

The Universal Primer site incorporated into the cDNA product 

enables profiling of any number of different microRNAs from a 

single cDNA reaction. The simple SYBR ® Green qPCR approach 

requires only a single microRNA-Specific Primer to work in 

conjunction with the Universal Primer to form a complete assay 

yielding a specific product. 

800 

400 

0 

miR-122 

1 1 

1,488 


miR-122 

2,000 

9,000 

6,000 

3,000 

0 

miR-124 

11,086 

2 1 


miR-124 

3.00 

2.50 

2.00 

1.50 

1.00 

0.50 

0.00 

miR-146a 


Contact your VWR Life Science Specialist for available 

assays, including: 

More than 900 human miR assays 

More than 300 mouse miR assays 

Human and mouse small RNA controls 


qScript microRNA cDNA Synthesis Kits 

25μL rxn 89168-788 200.00 

100μL rxn 

PerfeCTa SYBR Green SuperMix 

89168-790 700.00 

100x50μL rxn 101414-150 179.00 

500x50μL rxn 101414-152 809.00 

2,000x50μL rxn 101414-146 3,013.00 

PerfeCTa SYBR Green SuperMix w/ ROX 

100x50μL rxn 101414-158 179.00 

500x50μL rxn 101414-160 809.00 

2,000x50μL rxn 101414-154 3,013.00 

PerfeCTa SYBR Green SuperMix w/ Low-ROX 

100x50μL rxn 101414-166 179.00 

500x50μL rxn 101414-168 809.00 

2,000x50μL rxn 101414-162 3,013.00 

PerfeCTa SYBR Green SuperMix for iQ 

100x50μL rxn 101414-142 179.00 

500x50μL rxn 101414-144 809.00 

2,000x50μL rxn 101414-138 3,013.00 

1.00 

1.37 

miR-146a 

Figure 3, Accurate determination of microRNA expression profiles in human tissues: The relative abundance of individual microRNAs was examined 

in different human tissues. For each microRNA, the abundance in each tissue was expressed relative to the tissue with the lowest expression level. cDNA was 

prepared using the qScript microRNA cDNA Synthesis Kit and subsequent qPCR reactions performed using PerfeCTa ® microRNA Assays and SYBR ® Green 

SuperMix. For each microRNA the Cq (cycle to quantification) value for each tissue was subtracted from the Cq value of the tissue with the lowest Cq value 

(ΔCt). Relative abundance was then calculated as 2ΔCt. The relative expression of individual microRNAs in each tissue correlates well with published results 

(Liang, Y. et.al. 2007 BMC Genomics 8:166). 


2.4

NIST-Candidate RNA 

Control Products 

AIBioTech brings the first ever product for NIST Candidate Standard 

Reference RNA for next generation technologies to the marketplace. 

In partnership with the U.S. National Institute of Standards and Technology 

(NIST) and the External RNA Controls Consortium (ERCC), AIBioTech helped 

develop 96 Universal RNA standards which can serve as Standard Reference 

Material (SRM) for the industry. 

NIST-Traceable RNA controls are excellent for performance evaluation of 

reproducibility, sensitivity, and robustness in gene expression analysis 

or next generation sequencing studies. 

Description Cat. No. Each 

96 Tubes, 50μL Transcripts 89134-840 1,349.25 

Latin Square, 4 Tubes, 100μL Pools of 96 Transcripts 89134-842 2,398.67 

Dynamic Range, 4 Tubes, 100μL Transcripts 89134-844 3,598.00 

Custom Pool* 89134-846 4,722.38 

*Inquire with your VWR Life Science Specialist for details – 800.932.5000 


PCR Workstation with 

Stainless Steel Work Surface 

• Stainless steel work surface and a stainless steel ceiling angle reflector are 

used for greater UV reflection, resulting in a 10% increase in UV dose in 

PCR Workstations with a single UV bulb, and a 20% increase with a dual 

UV bulb 

• Stainless steel work surface is durable and cleans easily 

• Large interior workspace for PCR reaction supplies and equipment 

• Single or dual acrylic access panels close during UV irradiation, then slide 

into storage compartment in base during experimental procedures 

• Hinged glass door allows easy access to interior workspace for cleaning 

and placement of large instruments or supplies; Door is made from 

tempered safety-glass and blocks UV light 

• Accessory table and locking casters transform the PCR Workstation into a 

moveable work area that doesn’t infringe on valuable bench-top space 

• Single or Dual 254nm UV lamp with convenient switch for timed 

decontamination of workspace 

• Dual fluorescent lamps 

assure excellent work 

space visibility 

Dimensions 

PCR Workstations (DxHxW, in.) Cat. No. Each 

Single UV Light* 24x24x30 CBP-030-02-SS 1,646.00 

Dual UV Lights* 24x24x30 CBP-030-202-SS 1,708.00 

Single UV Light* 24x24x36 CBP-036-02-SS 2,020.00 

Dual UV Lights* 24x24x36 CBP-036-202-SS 2,115.00 

Single UV Light † 24x24x48 CBP-048-02-SS 2,525.00 

Dual UV Lights † 24x24x48 CBP-048-202-SS 2,625.00 

PCR Workstation Tables Dimensions 

For Use With (Width, in.) (Height, in.) Cat. No. Each 

30 28 CBT-030-28 715.44 

30 36 CBT-030-36 715.44 

36 28 CBT-036-28 746.32 

36 36 CBT-036-36 745.30 

48 28 CBT-048-28 766.91 

48 36 CBT-048-36 766.91 

* Single Door † Double Door 



Demonstration of FlashGel 

The sensitivity of the FlashGel System allows for a wide range of 

starting material concentrations. As with most gel recovery systems, 

the higher the concentration of starting sample, the higher the 

recovery yield. Intensity of recovered DNA from FlashGel Cassettes 

scales well with load levels of starting DNA, (Figures 1A and 1B). 

The FlashGel Recovery System is capable of recovering various size 

ranges and various size range windows within the sheared 

DNA (Figure 2). 

Recovery steps may be performed in parallel lanes to recover 

samples from multiple lanes, and multiple samples may be 

recovered from a single lane. Simply run the smallest of the desired 

size range in to the recovery well, stop the voltage, add FlashGel 

Recovery Buffer, and collect the sample. Then continue running the 

unwanted DNA through the recovery well, until the next desired size 

range reaches the well. Then stop the voltage, add more recovery 

buffer, and collect the next sample. 

Figure 1A: A dilution series of sheared DNA 600-37.5ng in amount 

and approximately 200-4,000bp in size were run on a double-tier 1.2% 

FlashGel Recovery Cassette at 275V for four minutes. Lanes QL contain 

FlashGel QuantLadder. 


Recovery with Sheared DNA 

More and more molecular biologists are working with sheared DNA. Sheared DNA is critical for constructing genomic libraries, and is utilized 

in DNA reassociation and hybridization analysis. Published methods for DNA fragmentation can be classified into four categories: sonication, 

enzymatic digestion, hydrodynamic shearing, and nebulization. Regardless of the fractionation method, gel electrophoresis is one of the best 

ways to estimate mean size and distribution of the DNA material 1 . 

The FlashGel System for Recovery is suitable for separating and recovering DNA, including fragmented DNA 2 . With the recent addition of a 

double-tier 2.2% concentration cassette format, it is possible to separate and recover a narrow window within a sheared DNA sample. 

DNA may be recovered in as little as five minutes, without the need for UV light or downstream purification. 

CAPABILITIES OF THE FLASHGEL SYSTEM FOR RECOVERY OF SHEARED DNA 

Sheared DNA on The FlashGel System 

Recovered Samples from Sheared DNA 

Figure 1B: 4µL aliquot of Figure 1A samples post recovery were run on 

a single-tier 1.2% FlashGel Cassette at 275V for five minutes. Lanes QL 

contain the FlashGel QuantLadder. Lane C contains 50ng of the original 

sheared DNA sample as a reference. Numeric lane labels indicate amount 

of starting material correlated from Figure 1A. 

Recovered Samples of Various Size Selections 

Figure 2: Recovered samples of various size selections from fragmented 

genomic lambda DNA. Lanes M contain the FlashGel 100-4,000bp Marker, 

Lanes QL contain the FlashGel QuantLadder, Lane C contains 50ng of Sheared 

DNA, Lanes 1-5 contain DNA extractions from several experiments taken from 

1.2% FlashGel Recovery Cassettes. Samples were run on a single-tier 1.2% 

FlashGel Cassette at 275V for seven minutes. 

Lane 1: 280-550bp; Lane 2: 650-1,190bp; Lane 3: 425-800bp; 

Lane 4: 425-800bp (smaller load volume of lane 3); Lane 5: 800-3,000bp

Sheared DNA Samples Following 

Size Selection Recovery 

1,500bp 

800bp 

400bp 

250bp 

QL 1 2 QL 

Figure 3: Lower MW fragments from two sheared 

DNA samples were recovered from a double-tier 

2.2% FlashGel Recovery Cassette. Several seconds of 

275V current were applied after the selected areas 

were removed from the samples. The images show 

the area where the recovered samples were 

removed. Samples of higher molecular weight are 

ready to be recovered. Lanes QL contain FlashGel 

QuantLadder. 

As long as current is applied, DNA that is not recovered will migrate 

through the well and leave no trace contamination (Figure 3). Only 

when the current is stopped, and the FlashGel Recovery Buffer is 

added, will DNA remain in the recovery well. Recovering DNA using 

the FlashGel System is highly efficient. Figure 3 shows clear voids 

where samples were removed during recovery. 

CAPABILITIES OF 1.2% & 2.2% FLASHGEL CASSETTES 

Extraction range windows are predefined by selecting and using the 

1.2% or the 2.2% Recovery Cassettes. Optimal separation range for 

the 1.2% FlashGel Recovery Cassettes is 50-4,000bp, and optimal 

separation range for the 2.2% Recovery Cassettes is 10-1,000bp. 

The 2.2% cassettes provide a larger spread of bands in the 10-400bp 

range, while the 1.2% cassettes show a wider spread in the larger 

size range (Figure 4). The 2.2% cassettes will provide a tighter and 

more defined range than the 1.2% cassettes within the same size 

range (Figure 5). The 1.2% cassettes may be better suited for a 

wider size selection range in a single extraction cycle. 

SUMMARY 

The FlashGel System is an efficient tool for fragmented DNA size 

selection. The two recovery cassette concentrations (1.2% and 

2.2%) are optimized to provide narrow separation over a wide size 

range. With the FlashGel System, DNA fragments may be separated, 

recovered, and photographed in as little as five minutes, without 

the use of UV light. Recovered samples are immediately ready for 

downstream applications– eliminating agarose gel preparation, 

band excision, and purification. 

Demonstration of 

Size Separation 

~150bp 

100bp 1,500bp 

800bp 

400bp 

250bp 

Figure 4: Sheared DNA consisting of fragments 

50-3,000bp run on 1.2% and 2.2% FlashGel 

Cassettes. Lane QL is FlashGel Quant Ladder, 

lane 1 is the DNA. 

100bp 


Demonstration of 

Size Recovery 

FlashGel Cassette 2.2% FlashGel Cassette 

1.2% 2.2% 

QL 1 1 QL QL 1 2 3 4 

Figure 5: Lanes labeled 1 and 3 are extraction 

of samples through recovery well window from 

1.2% and 2.2% cassettes. Lanes 2 and 4 are slightly 

higher load volumes of Lanes 1 and 3 respectively. 

Lane QL contains the FlashGel QuantLadder. 

REFERENCES 

1. Ordahl CP, Johnson TR, Caplan AI. (1976). Sheared DNA Fragment Sizing: 

Comparison of Techniques. Nucleic Acids Res. 3(11):2985-99. 

2. Mary Riley and Hugh White. (2009). FlashGel System for DNA Recovery. ResourceNotes. 

6(1): 17-20. White paper available on line: www.lonza.com/go/literature/2212. 

3. (2009). FlashGel System - Protocol. Document # 00521123-0209-1. 

Available online: www.lonza.com/go/literature/1657. 


FlashGel Recovery Starter Kit contains: 

• FlashGel Recovery Cassettes, 1.2%, 

8+1 (18-well), Double Tier (Pk. 9) 

95053-314 Ea./ 416.00 

• FlashGel Loading Dye (5x1mL, 5X Concentrate) 

• FlashGel Recovery Buffer, Ready-to-Use 

(2x500μL) 

• FlashGel QuantLadder (100bp (3ng) - 

1.5kb (30ng) (50 apps, 250μL) 

• Visualization Glasses 

• Control Fragment 

FlashGel Recovery Cassette, 

1.2%, 8+1 (18-well), Double Tier 

95053-310 Pk. 9/ 105.00 

FlashGel Recovery Cassette, 

2.2%, 8+1 (18-well), Double Tier 

89135-718 Pk. 9/ 105.06 

FlashGel Recovery Buffer (2x500μL) 95053-312 Ea./ 134.00 

FlashGel System contains: 

• FlashGel Dock 

• FlashGel Camera 

95045-604 Ea./ 1,025.00 

• FlashGel DNA Cassettes, 1.2%, 12+1 well, Single Tier (Pk. 9) 

• FlashGel Loading Dye and DNA Marker 



EvaGreen ® 

qPCR Dye KingFisher ® 

4 

3 

2 

1 

Fast EvaGreen ® qPCR Master Mix 

Compared to SYBR ® Green qPCR Master Mix from Company A 

and Company Q qPCR using 20pg to 200ng Template 

● Fast EvaGreen 

----- Fast EvaGreen NTC* 

---–--- Company A 

----- Company A NTC* 

---–--- ▲ Company Q 

----- Company Q NTC* 

*No template control 

0 

15 20 25 30 35 40 

Next-generation DNA dye for quantitative real-time PCR (qPCR) binds DNA 

by a novel “release-on-demand” mechanism that allows the use of a high 

dye concentration to maximize fluorescence signal while minimizing PCR 

inhibition. This allows for superior sensitivity, melt curve analysis, and high 

resolution melt curve analysis (HRM)*. 

• Does not penetrate cell membranes – no interaction with DNA in live cells 

• Non-cytotoxic and non-mutagenic 

• Detailed safety report available – contact your VWR Life Science Specialist 

• Unrivaled melt-curve analysis 

Fast Plus EvaGreen Master Mix features Biotium’s chemically modified 

Cheetah hot-start Taq polymerase, and is available with or without 

Rox reference dye for qPCR. 

* Performing HRM may require a 

license from Idaho Technologies 

Description Qty. Cat. No. Price 

Fast Plus EvaGreen qPCR Master Mix, Low Rox (1mL) 

Trial Size, 100 rxn 1mL 89139-032 61.80 

200 rxn 2x1mL 89139-026 129.78 

500 rxn 5x1mL 89139-028 283.25 

5,000 rxn 50x1mL 89139-030 2,575.00 

Fast Plus EvaGreen qPCR Master Mix, High Rox (1mL) 

Trial Size, 100 rxn 1mL 89139-040 61.80 

200 rxn 2x1mL 89139-034 128.75 

500 rxn 5x1mL 89139-036 283.25 

5,000 rxn 

EvaGreen Dye (1mL) 

50x1mL 89139-038 2,575.00 

20X in Water, Trial Size 1mL 89138-984 37.85 

20X in Water 5x1mL 89138-982 162.23 


Nucleic Acid 

Purification Kits 

Unique High Throughput Workflow 

KingFisher Nucleic Acid Purification Kits have been designed and optimized 

for use with the Thermo Scientific KingFisher magnetic particle processors, 

providing a unique automated workflow. Enabling the fast and accurate 

purification of DNA and RNA, the kits contain all relevant buffers and 

reagents for a range of different sample types. As a unique, open, and 

flexible instrument, the KingFisher system enables users to select a kit that 

best meets their specific application demands. As a result, you can employ 

a single instrument for a broad range of protocols. Therefore, the KingFisher 

Nucleic Acid Purification Kits enable you to create a complete workflow, from 

sample preparation to downstream application analysis, from a single source. 

• Flexible KingFisher system with optimized purification kits 

• Customized kits for wide variety 

of sample types 

Description 

KingFisher Flex Kits, 1x96 Preps 

Cat. No. Price 

Blood DNA 97060-408 242.11 

Cell & Tissue DNA 97060-416 254.24 

Plant DNA 97060-422 237.03 

Total RNA 97060-412 265.10 

Viral Nucleic Acid 

KingFisher mL Kits, 60 Preps 

97060-420 249.51 

Blood DNA 97060-406 237.94 

Cell & Tissue DNA 97060-414 245.04 

Total RNA 97060-410 255.23 

Viral Nucleic Acid 97060-418 188.55

illustra 

GFX 

PCR DNA 

and Gel Band Purification Kits 

Designed for the rapid purification and 

concentration of PCR products or DNA 

fragments. Can be used to purify DNA from 

reaction volumes up to 100μL, or agarose 

gel slices up to 900mg. Typical recoveries range 

from 60 to 80% for DNA fragments from 

agarose gel, to as high as 95% for PCR products 

from solution; 99.5% of contaminants removed. 

• For the isolation and concentration of DNA 

fragments (50bp to 10Kb) from PCR mixtures, 

DNA-containing agarose gel bands, enzymebased 

DNA modifications, and restriction digestions 

• Fast and easy-to-use method with less than 

10 minutes hands-on time 

• Flexible 10 to 50μL elution volume 

for different DNA concentration needs 

• Visual color indicator to the capture buffer 

ensures optimal pH for maximum DNA 

binding and recovery 

No. of Purifications Cat. No. Price 

100 95026-728 170.00 

250 95026-730 400.00 

10x96-well Plates 95026-726 1,345.00 

PyroScript ® 

RT-PCR 

2X Master Mix 

One Step, One Enzyme, One Master Mix 

Enables thirty minute reverse transcription-PCR amplification 

of RNA up to 400bp in length without a separate reverse 

transcription step. Unique thermostable PCR enzyme 

with efficient reverse transcription activity streamlines the 

process by reducing hands-on time and the possibility for error. 

Just add template, primers, and cycle. Detect amplification products 

by gel electrophoresis or real-time analysis. Extreme thermostability 

of the enzyme allows denaturation of crude specimens at 94°C to 

simplify sample preparation. Reverse transcription at 70°C, or even 

higher, improves analysis of highly-structured RNA templates. 

• Fast - Amplify viral and cellular RNA in 30 minutes 

• High Temperature - 

Reverse transcription at > 70°C 

• Convenient - Just add 

primers and RNA template 

PyroScript RT-PCR 2X Master Mix Kit Cat. No. Price 

50 rxns 89137-906 125.00 

100 rxns 89137-908 225.00 

500 rxns (5x100) 89137-910 999.00 


Gel/PCR DNA Fragments Kits 

IBI Standard Gel/PCR DNA Fragments Kits are designed to recover or 

concentrate DNA fragments from 50bp to 10Kb using a chaotropic salt 

to dissolve the agarose and denature the enzymes. The DNA fragments in 

the chaotropic salt are bound to the glass fiber matrix of the spin column. 

Contaminants are washed with a wash buffer and the purified DNA is then 

eluted from the column, with recovery rates around 80 to 90%. 

IBI Gel/PCR DNA Purification Kit for small DNA fragments is specifically 

designed to recover or concentrate DNA fragments of 50 to 200bp from 

agarose gel or other enzymatic reactions utilizing an easy, 20 minute 

protocol, as well as provide excellent yield and purity. 

• Easy to use standard protocols 

• Excellent yield and purity 

• Save up to 45% on your sample prep costs 


Gel PCR DNA Fragments Extraction Kits 

4 Preps 95039-640 Ea./ 7.14 

100 Preps 95039-642 Ea./ 110.16 

300 Preps 95039-644 Ea./ 333.54 

Small DNA Fragments Extraction Kits 

4 preps 97060-556 Pk. 2/ 7.14 

100 Preps 97060-558 Pk. 2/ 107.10 

300 Preps 97060-560 Pk. 2/ 328.44 



Gel Imagers 

Basic Gel Imaging With Choice of Options 

Capture and print images with this easy to use, 

compact imager. Users can see fluorescent 

gels through the unique viewport 

window. The window blocks UV and 

allows users to safely see gels when 

illuminated by the UV 

transilluminator. Save images 

to the memory card or connect 

to a PC to transfer the images. 

System Includes: 

Monochrome CCD camera 

Lightweight hood with handles 

Ethidium bromide filter 

Choice of transilluminator, 

camera, and door configuration 

Additional models available 

Ask your VWR Sales Representative 

about accessories including: 

thermal printer and paper, 

converter plates (converts UV to 

white light for Coomasie Blue 

and Silver Stains), gel cutter 

and gel tools, and analysis software. 

See www.vwrsp.com/imagers 

for the complete product line. 

Description Wavelength, nm Cat. No. Price 

Gel Imager, 0.3 MP Camera, 115V 

20x20cm 302 89131-380 7,117.94 

20x20cm 302/365 89131-388 7,301.10 

21x26cm 302 89131-384 7,321.45 

21x26cm 302/365 89131-392 7,504.62 

Gel Imager Plus, 1.3 MP Camera, Side Access Doors, 115V 

20x20cm 302 89131-396 8,115.16 

20x20cm 302/365 89131-404 8,318.68 

21x26cm 302 89131-400 8,318.68 

21x26cm 302/365 89131-408 8,522.19 


Green PCR Reagents 

Environmentally Friendly 

GenScript’s advanced high-stability technology has led to the invention of a 

new generation of PCR reagents– Green PCR Reagents. The Green series of 

reagents, from the proprietary Taq, dNTP, to PCR mixtures, are stable for one 

month if stored at room temperature, or for over six months if stored at 4°C. 

The high-stability feature comes with increased half-life of active enzymes at 

high temperatures, which results in more amplification cycles and higher yield. 

• No freeze-thaw cycles provide 

instant access to high-throughput 

PCR automation 

• Helps preserve energy associated 

with storage and shipping 

• No contamination activity has been 

detected in standard test reactions 

• Enhanced yields with longer 

enzyme half-life 

• Stable at room temperature – 

stop refrigerating and save energy 


Green Taq DNA Polymerase 1,000U 95055-024 61.80 

Stabilized dNTP Mix, 10mM 0.5mL 95057-806 29.86 

UV, Dual UV, and Variable 

Intensity Transilluminators 

• Uniquely designed with 

increased UV intensity 

and uniformity 

• Instant-on capabilities 

• No lamp flicker 

• Reduced electrical 

consumption 

Visit www.vwrsp.com/imagers 

for a complete listing of sizes 

and options. 

89131-452 

UV Transilluminator Filter Size, cm Cat. No. Price 

302nm UV 20x20 89131-440 1,379.00 

302nm UV 21x26 89131-444 1,617.00 

Dual, 302/365nm UV 20x20 89131-464 1,670.00 

Dual, 302/365nm UV 21x26 89131-468 1,935.00 

Variable Intensity, 302nm UV 20x20 89131-452 1,431.00 

Variable Intensity, 302nm UV 21x26 89131-456 1,670.00

Multiskan ® 

GO UV/Vis 

Spectrophotometer 

Excellent for DNA/RNA Assays 

with Both Microplates and Cuvettes 

The Thermo Scientific Multiskan GO microplate 

spectrophotometer reads 96- and 384-well 

plates and cuvettes. A broad wavelength range 

including UV as well as pathlength correction 

and fast reading speed make it an ideal tool 

for any photometric research application, 

including DNA/RNA and protein analysis. 

Excellent measurement precision guarantees 

excellent sensitivity in DNA and RNA 

measurements. The Multiskan GO offers 

true usability both as standalone or in PC 

control. When high throughput is required, 

quantitative and qualitative DNA assays can 

be run in 384-well plate format, and low 

throughput needs are fulfilled with simple 

ready-made cuvette sessions. DNA spectrum 

measurement is also a valuable tool for fast 

analysis of the sample quality. 

• Stand-alone use for quick and simple 

cuvette and plate measurements 

• Easy assay setup for demanding assays 

with PC control 

• Ready-made cuvette session for 

DNA/RNA concentrations and purity 

Multiskan GO accepts 

versatile cuvettes. 

Measurement data can 

easily be saved on a USB 

memory stick for transfer 

to a computer for 

further processing. 


Multiskan GO, w/ Cuvette 97051-718 12,257.00 

Multiskan GO, w/o Cuvette 97051-716 10,197.00 

Blocking Solutions, 

Buffers, and Substrates 


Founded in 1962, Rockland manufactures over 5,000 antibodies and 

antibody-based tools in its laboratories near Philadelphia, PA. Antibodies 

are in the areas of Cell Signaling, Cytokines, Notch Signaling, NFkB 

Pathway, PI3 Kinase Pathway, Ubiquitin, and UBL Proteins. 

• Sensitive 

• Quality made 

• 100% guaranteed 

Description 

Blocking Solutions 

Size Cat. No. Price 

BLOTTO Blocking Agent 500g RLB501-0500 50.00 

Bovine Serum Albumin (BSA) 

Fraction V 

50g RLBSA50 80.00 

Bovine Serum Albumin 30% 

Solution (BSA 30%) 

500mL RLBSA-30 385.00 

ELISA Microwell Blocking 

Buffer & Stabilizer 

1L RLMB-064-1000 155.00 

Fluorescent Western Blot 

Blocking Buffer 

500mL RLMB-070 115.00 

Goat Serum (NGS) 10mL RLB304 90.00 

IHC Blocking Buffer 100mL RLMB-071-0100 85.00 

Mouse Serum (NMS) 

Buffers 

10mL RLB308 225.00 

EDTA, 0.5M, pH 8.0 100mL RLMB-014 50.00 

PBS, 10X, pH 7.2 1L RLMB-008 50.00 

SDS-PAGE Running Buffer, 

10X, pH 8.3 

1L RLMB-017 60.00 

TAE, 50X, pH 8.3 1L RLMB-020 75.00 

TBS, 10X, pH 7.5 

Substrates 

1L RLMB-012 60.00 

ABTS Liquid Peroxidase 100mL RLABTS-100 80.00 

BCIP/NBT Liquid Alk. Phos. 100mL RLNBT-100 80.00 

Chemiluminescent FemtoMax 

Super Sensitive 

110mL RLFEMTOMAX-110 295.00 

TMBE Liquid Peroxidase 1L RLTMBE-1000 175.00 



Mouse Genotyping in Less Than One Hour 

KAPA Kits for Rapid Extraction and Amplification of DNA from Mouse Tissue 

KAPA Mouse Genotyping Kits Contain: 

• KAPA Express Extract - 15 minute extraction using no 

organic solvents and requiring no centrifugation or 

pH neutralization. 

• KAPA2G Fast Genotyping Mix - 45 minute amplification 

using a novel DNA polymerase engineered for high 

processivity and extreme speed. 

• Available in a convenient master mix format with loading 

dye, in both hot start and non-hot start formulations. 

Template DNA for mouse genotyping is routinely prepared from ear or 

tail biopsy samples. Typically, samples are incubated for several hours 

or overnight with Proteinase K, followed by purification of the DNA to 

remove salts and detergents. Alternatively, DNA is released from the 

mouse tissue by heating the sample in an alkaline solution, followed 

by neutralization in a Tris-HCl buffer with an acidic pH. Proteinase K 

protocols yield extracts with a higher DNA concentration and quality, 

but are laborious and time-consuming. Heat lysis protocols allow for 

rapid generation of PCR-ready DNA, but samples vary in quality and 

the concentration of amplifiable template, leading to reduced PCR 

success rates with wild-type DNA polymerases. 

KAPA Biosystems offers a unique solution for mouse genotyping. 

KAPA Mouse Genotyping Kits contain KAPA Express Extract for 

DNA extraction and KAPA2G Fast Genotyping Mix for DNA 

amplification. KAPA Express Extract DNA Extraction Kits contain a 

novel thermostable protease and buffer system designed for rapid and 

efficient, single-tube mouse tissue lysis. Good quality, PCR-ready DNA 

can be generated in as little as 15 minutes with minimal handling, 

thereby reducing the risk of sample loss or contamination. The 

process yields sufficient template for multiple assays and is easily 

scaled to handle samples in a 96-well format. KAPA2G Fast 

Genotyping Mix with dye is ideally suited for the fast and reliable 

amplification of DNA fragments across a wide range of amplicon 

lengths and GC contents. This ready-to-use cocktail contains KAPA2G 

Fast DNA polymerase, a novel enzyme engineered for improved 

processivity and speed. Together, these reagents allow for significantly 

reduced turnaround times and improved success rates in mouse 

genotyping (Figure 1). 


Total reaction time (hours) 

6.0 

5.0 

4.0 

3.0 

2.0 

1.0 

0.0 

1.0 

KAPA Mouse 

Genotyping Kit 

5.0 

Proteinase K + 

Wild-type Taq 

4.0 

Alkaline lysis + 

Wild-type Taq 

Figure 1: The KAPA Mouse Genotyping Kit outperforms crude DNA 

extraction methods used for mouse genotyping, in significantly shorter 

turnaround times. Results for five amplicons (312-915bp) generated with 

the KAPA Mouse Genotyping Kit (1) were compared to those obtained 

with two commonly used methods (2 and 3) (bottom panel). With the 

KAPA Mouse Genotyping Kit, DNA lysates were prepared from mouse tails 

with the rapid (15 minutes), single-tube KAPA Express Extract system. 

Amplification with the KAPA2G Fast Genotyping Mix with dye was 

completed in 45 minutes. In contrast, DNA lysates were prepared with 

a ~3.5-hour Proteinase K protocol (2) or a rapid (~2.5-hour) alkaline 

lysis method (3) (top panel). In both cases, amplification was performed 

with wild-type Taq (1.5-hour cycling protocol). Results obtained with the 

KAPA Mouse Genotyping Kit were equal or better (more specific) than 

those obtained with other methods, which (depending on the exact DNA 

extraction protocol used) may take at least four times as long, or up to 

one day to complete.

From Tail to Type in One Hour 

Tail or 

Ear Tissue 

Incubation at 

75°C and 95°C 

Fast PCR 

Gel Electrophoresis 


PRODUCT OVERVIEW 

Current workflows for the extraction and amplification of DNA for mouse 

genotyping can benefit from improvements in throughput, turnaround 

time and reliability. 

KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel 

thermostable protease and buffer system that allows the extraction of 

PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G 

Fast Genotyping Mix with dye, containing a DNA polymerase engineered 

via a process of molecular evolution for high processivity and extreme 

speed. The combination of KAPA Express Extract and KAPA2G Fast 

Genotyping Mix allows for the reliable extraction and amplification of 

DNA fragments from mouse tissue in 1 hour, as compared to ≥1 day 

with conventional protocols. 

The KAPA Express Extract system has been designed for optimal tissue 

lysis and sample preservation. Unlike existing protocols that rely on 

proteinase K digestion, extractions using KAPA Express Extract are 

conveniently performed in a single-tube, without the need for hazardous 

chemicals and multiple washing steps. Extracted DNA is then amplified 

with KAPA2G Fast Genotyping Mix (2X) with dye, a ready-to-use master 

mix containing all components for Fast PCR, except primers and 

template. The master mix is designed for routine Fast PCR and improved 

performance, compared to wild-type Taq DNA polymerase. The master 

mix with dye additionally contains two inert dyes to allow for the analysis 

of reaction products by gel electrophoresis directly after PCR, i.e. without 

needing to add a DNA loading solution. 


KAPA Mouse Genotyping Kit 500 rxn 97060-478 450.00 

KAPA HotStart Mouse Genotyping Kit 500 rxn 97060-480 500.00 

KAPA2G Robust HotStart DNA Polymerase 

2G Robust HS ReadyMix 100 rxn 89125-076 89.28 

2G Robust HS ReadyMix 500 rxn 89125-078 341.38 

2G Robust HS Enzyme Kit 250 U 89125-064 157.55 

2G Robust HS Enzyme Kit 500 U 89125-066 288.86 

KAPA2G Fast HotStart DNA Polymerase 

2G Fast HS ReadyMix 100 rxn 89125-110 52.52 

2G Fast HS ReadyMix 500 rxn 89125-112 196.95 

2G Fast HS Enzyme Kit 250 U 89125-090 144.42 

2G Fast HS Enzyme Kit 500 U 89125-092 199.57 

KAPA HiFi HotStart DNA Polymerase 

HiFi HS ReadyMix 100 rxn 89125-040 126.05 

HiFi HS ReadyMix 500 rxn 89125-042 525.20 

HiFi HS w/dNTP Mix 250 U 89125-036 110.29 

HiFi HS w/dNTP Mix 500 U 89125-038 262.60 

KAPA HRM Fast PCR Kits, Size 

1mL – 97060-314 75.00 

5mL – 97060-316 325.00 

10mL – 97060-318 600.00 



Hazard Reducing RNA Kits RNA Purification Dyes 

and Solutions 

Phenol-Free Total RNA Purification Kit 

1.5% Formaldehyde Agarose Gel 

Formaldehyde-Free RNA Gel Kit 

30 

Amresco ® Competitor 

Agilent BioAnalyzer Gel Image 

Amresco ® Competitor 

Small RNA 

Species 

Size range of RNA purified with Phenol-Free Total RNA Purification Kit versus 

a competitor’s kit. Note the presence of small RNA species (red circles) in the 

samples isolated via Phenol-Free Total RNA Purification Kit and the absence 

of these species in the competitor RNA preparation. 

Purifies high quality RNA in just 

20 minutes 

No additional staining or 

destaining steps 

Ethidium Bromide Formaldehyde-Free RNA Dye 

25 20 15 10 5 30 25 20 15 10 5 

Total RNA µm Total RNA µm 

Denatured in Formaldehyde-Free RNA loading buffer containing either 

ethidium bromide or Formaldehyde-Free RNA dye and incubated 10 minutes 

at 65°C. Formaldehyde-Free RNA gel dye is as sensitive as ethidium bromide. 

All-in-one RNA gel kit produces 

immediate results without 

hazardous chemicals 

No formaldehyde or 

ethidium bromide 

One convenient solution 

Non-toxic, non-mutagenic, 

alternative to ethidium bromide 

No long incubations or 

buffer recirculation 


Phenol-Free Total RNA 

Purification Kit 

50 preps 97064-308 178.00 

Formaldehyde-Free RNA Gel Kit 

Visit vwr.com for Kit contents. 

15 gels 97064-300 179.90 


RiboReserve RNA Storage Solution stabilizes RNA for long term 

storage and is sterile and RNase-free – will not inhibit downstream RT-PCR, 

transcription, or Northerns. 

RNA EZ-Vision ® and EZ-Vision ® RNA Ladder provide instant visualization 

with non-mutagenic, fluorescent dye in loading buffer – no post staining, 

destaining, or ethidium bromide. 

Gold-N-Gel RNA Stain (200X) is a non-mutagenic, in-gel fluorescent 

stain for instant RNA visualization – no post staining, destaining, or 

ethidium bromide. 


RiboReserve RNA Storage Solution 12x1mL 97063-258 49.37 

RiboReserve RNA Storage Solution 50mL 97063-260 31.00 

RNA EZ-Vision Dye as Loading Buffer 2x1.5mL 97064-294 89.00 

EZ-Vision RNA Ladder 150μL* 97063-358 172.00 

Gold-N-Gel RNA Stain, 12.5mL 97064-304 88.25 

30x100mL gels 

* Includes 7 bands ranging from 1,500 nt - 9,000 nt, sufficient for 25 lanes 

ExiSpin 

The World’s First Combination 

Vortex/Centrifuge 

Convenience & Time Savings 

ExiSpin provides reproducible results using optimized 

vortexing of multiple samples. Four 8-strip 0.2mL tubes 

can be handled simultaneously enabling up to 32 tubes 

to be run at once. Or use the 12-place rotor (included), to 

load twelve 1.5mL tubes simultaneously for cell lysis and other applications. 

Programmable speed and time guarantee the reproducibility of each 

application. Use it for minipreps to resuspend oligos, set up your PCR 

reactions, or any task that requires vortexing and a subsequent spin-down. 

Once you try the ExiSpin, you’ll find that it’s an essential instrument in 

your laboratory. 

• Fully automated spin-vortex-spin centrifuge 

• Can be used as a centrifuge, 

a vortex, or both 

• Rotors for microcentrifuge tubes 

and strip tube included 


ExiSpin Vortex/Centrifuge 89170-878 870.00 

PCR PreMix 95027-448 52.00 

HotStart PCR PreMix 95027-520 73.00 

TLA High Fidelity/Long Range PCR PreMix 95027-510 79.00

AcroPrep 

Advance Filter 

Plates for Ultrafiltration 

AcroPrep Advance Filter Plates are a versatile format to advance your 

sample prep. High performance membrane housed in optimized filter plate 

configurations meet a spectrum of sample prep requirements. Our filter plates 

for ultrafiltration provide rapid, efficient separation of biomolecules. 

• Omega membrane provides high recovery – typically results in greater 

than 90% 

• New well design results in faster, more consistent filtration rates across 

the plate 

• Applications include size exclusion, PCR clean-up, nucleic acid purification, 

and protein separation 

MWCO/Pore Size 

Filter Plates with Omega, 350µL 

Cat. No. Pack of 10 

3K 97052-106 332.92 

10K 97052-108 332.92 

30K 97052-110 343.92 

100K 97052-112 343.92 

Enzyme Immunoassay 

Kits and Peptides 

Bachem. Quality Matters. 

Proteomics 

Bachem is pleased to offer 

peptide, protein, and 

immunoassay products to 

the life science community 

that are manufactured to 

unmatched standards of 

quality and reliability. Our 

Enzyme Immunoassays 

(EIA) are highly sensitive, 

highly specific, and 

non-radioactive, making 

them cost-effective and easy to use. Bachem’s peptides and complementary 

immunology products are indispensable tools used in a wide variety of life 

science fields of study including apoptosis, diabetes, cardiovascular diseases, 

neuroscience, obesity, osteoporosis, and pituitary dysfunction. 

• High Purity Reagents – Set the standard for quality and reliability – 

the benchmark in consistent purity and solubility 

• Convenient – Peptides, proteins, and immunoassay kits available from 

stock in convenient pack sizes 

• Comprehensive – The widest selection of bioactive peptides and proteins 

currently available, and a complementary immunology product line 

Description 

High Sensitivity EIA Kits, Rabbit Host 

Cat. No. Each 

ß-MSH (Human) S-1426.0001BA 535.00 

Adrenomedullin (Rat) S-1423.0001BA 535.00 

Alarin (Rat) S-1424.0001BA 535.00 

Copeptin (Human) S-1427.0001BA 550.00 

Copeptin (Rat) S-1422.0001BA 535.00 

Maxadilan S-1425.0001BA 565.00 

Pancreastatin, 33-48 (Human) 

Peptides 

S-1421.0001BA 535.00 

ß-MSH (Human) H-1475.0001BA 235.00 

Adrenomedullin (Rat) H-2934.0500BA 431.00 

Copeptin (Human) H-6718.0500BA 281.00 

Copeptin (Rat) H-6726.0500BA 281.00 

Maxadilan H-6734.0500BA 909.00 

Pancreastatin, 33-48 (Human) H-6506.0001BA 346.40 


Proteomics 

Spin-X ® 

Spin-X UF 500 for 

samples up to 500µL 

UF Concentrators 

Corning ® Spin-X UF Centrifugal Concentrators offer a simple, one-step 

procedure for concentrating or desalting proteins and other biomolecules 

with 90% or better recovery. The vertical membrane design and thin channel 

filtration chamber minimizes membrane fouling and provides fast, high-speed 

concentrating, even with particle-laden solutions. Integrated dead-stop design 

eliminates risk of spinning to dryness; no respinning necessary. 

Choice of three sizes for greater flexibility: 

Spin-X UF 6 for 

samples up to 6mL 

Spin-X UF 20 for samples 

up to 20mL, (14mL if 

using fixed angle rotors) 

Low binding polyethersulfone (PES) membranes are available with five 

molecular weight cut-offs (MWCO): 5,000, 10,000, 30,000, 50,000, and 

100,000 to meet all of your concentrating needs. Choose a MWCO 

1 1 

⁄2 to ⁄3 smaller than the protein to be concentrated. 

The MWCO and graduations are printed right on the side of the concentrator 

tube to avoid mix-ups. Spin-X UF 6 and 20 concentrators can be used with 

either swinging bucket or fixed-angle rotors. Spin-X UF 500 concentrators 

require fixed-angle rotors. 

MWCO 

Spin-X UF 500, 500µL 

Cat. No. Case of 

5,000 97027-888 25/ 83.29 

10,000 97027-890 25/ 83.29 

30,000 97027-892 25/ 83.29 

50,000 97027-894 25/ 83.76 

100,000 

Spin-X UF 6, 6mL 

97027-896 25/ 83.76 

5,000 97027-898 25/ 113.10 

10,000 97027-900 25/ 113.10 

30,000 97027-902 25/ 113.10 

50,000 97027-904 25/ 113.10 

100,000 

Spin-X UF 20, 20mL 

97027-906 25/ 113.10 

5,000 97027-908 12/ 102.82 

10,000 97027-910 12/ 102.82 

30,000 97027-912 12/ 102.82 

50,000 97027-914 12/ 102.82 

100,000 97027-916 12/ 102.82 


SuperSignal ® 

Western 

Blot Enhancer 

Increase Signal-to-Noise Ratio and Band 

Development for Better Sensitivity 

The Thermo Scientific SuperSignal Western Blot Enhancer contains a 

membrane treatment reagent and a primary antibody diluent that increase 

both signal intensity and sensitivity 3- to 10-fold compared to detection 

performed without it. 

When a protein or antigen is difficult to detect because of low abundance 

or poor immunoreactivity, use of SuperSignal Western Blot Enhancer can 

significantly reduce background and enhance detection of low-abundance 

and weakly immunoreactive antigens. This versatile kit works with PVDF and 

nitrocellulose membranes, and is compatible with fluorescent, chromogenic 

and chemiluminescent detection. 

Increase Sensitivity – Achieve 3- to 10-fold increase in signal intensity 

and sensitivity 

Improve Specificity – Improves signal-to-noise ratio for poor quality 

and low affinity antibodies 

Better Clarity – Reduces background for cleaner Western blots 

No. Mini Blots Cat. No. Price 

2 PI46641 41.12 

25 PI46640 204.57

Kodak 

BioMax ® 

Film – 

See the Difference! 

Enhanced visualization so that even 

the faintest signals can be seen with 

BioMax’s exclusive clear film base. 

Kodak has the widest selection of 

film types to provide sensitivity, speed, 

and contrast designed for specimens 

tagged with all commonly used markers. 

BioMax Light Film 

Maximum clarity and sensitivity 

Highest signal-to-noise ratio results 

with all chemiluminescent kits 

BioMax MR Film 

Two-fold increase in sensitivity in the detection of 35S, 33P, and 

14C labeled samples 

Fast quenching chemiluminescent-tagged specimens 

Time to properly load your cassettes and deliver images with 

unsurpassed resolution 

BioMax XAR Film 

Exceptional results for most radio and chemiluminescent-labeled samples 

Most versatile scientific film available today 

BioMax MS Film 

Maximum sensitivity for light emitted from BioMax MS Intensifying Screens 

Ideal for Northern, Southern, and dot blotting applications 

Suitable for low intensity detection or reduced exposure time 

Most sensitive detection system available 

For other Kodak film options, 

please visit vwr.com. 

Dimensions, cm Cat. No. Pack of 50 Case of 5 Packs 

BioMax Light 

20.3x25.4* IB-1788207 245.79 – 

13x18 † IB-1917012 386.44 1,469.58 

BioMax MR 

20.3x25.4* IB-IB8701302 403.59 1,534.79 

13x18* IB-8941114 199.52 – 

BioMax XAR-2 

20.3x25.4• IB1651454 375.04 1,426.20 

13x18• IB1651496 251.30 955.63 

BioMax MS 

20.3x25.4* IB-8294985 403.59 1,534.79 

13x18* IB-1111681 262.70 999.01 

* Non-Interleaved † Individually Wrapped • Alternate-Interleaved 

Ordering Information: Alternate-interleaved sheets are packed with 

sheets of paper to protect film. 

Proteomics 

pH Test Strips and 

DoubleZone Indicator Papers 

pH Test Strips 

BDH ® pH Test Strips are a high 

quality, color-fixed pH test strip. They 

are always ready for immediate use, and 

as single use devices, they are completely 

maintenance free. 

Designed for safe testing of dangerous, poisonous, or aggressive liquids 

Longest available handle protects user from contact with sample 

Fixed dye cannot bleed into the sample, protecting it against 

contamination and enabling measurements even in weakly buffered 

or strongly alkaline solutions 


For each pH value, these papers show a single 

color that can be matched with the color scale at 

intervals of 0.2 to 1pH. They are also able to show 

two different colors for each pH value at intervals of 

0.3 to 1pH unit for even more accurate reading and 

better estimation of intermediate values. The colors 

on the color scales are specially mixed to perfectly 

match the reaction color of the indicator papers to 

make the read-off of results easy and accurate. 

The BDH DoubleZone Indicator Papers are 

10mm wide and come in 5m long rolls. 

Description pH Graduation Cat. No. Each Pack of 5 

Universal Range Test Strips 

0-14.0 1.0 BDH35309.606 13.00 63.33 

Intermediate Range Test Strips 

0-6.0 0.5 BDH35310.601 13.00 63.33 

2.0-9.0 0.5 BDH83930.601 13.00 63.33 

4.5-10.0 0.5 BDH35311.604 13.00 63.33 

6.0-10.0 0.3/0.4 BDH83931.601 13.00 63.33 

7.0-14.0 0.5 BDH35312.607 13.00 63.33 

Narrow Range Test Strips 

0.3-2.3 0.3/0.4 BDH35314.604 13.00 63.33 

1.7-3.8 0.3 BDH35315.607 13.00 63.33 

3.6-6.1 0.3/0.5 BDH35316.601 13.00 63.33 

5.1-7.2 0.3 BDH83932.601 13.00 63.33 

6.0-7.7 0.3/0.4 BDH35317.604 13.00 63.33 

7.5-9.5 0.2/0.4 BDH35318.607 13.00 63.33 

7.9-9.8 0.2/0.4 BDH83933.601 13.00 63.33 


1.0-4.3 0.5 BDH35301.600 15.00 70.00 

1.0-12.0 1.0 BDH35300.606 15.00 70.00 

3.5-6.8 0.5 BDH35302.603 15.00 70.00 

5.0-8.0 0.5 BDH35303.606 15.00 70.00 

7.0-10.0 0.3/0.4 BDH70035.607 15.00 70.00 

9.5-14.0 0.5 BDH70036.601 15.00 70.00 


Microbiology 

UV Crosslinker 

Multipurpose Ultraviolet Exposure 

The VWR ® Crosslinker’s overhead 

254nm UV is used for sterilization 

and decontamination of plates, 

small tools, etc. The Crosslinker 

assures consistent UV output 

for many applications. 

• Flexibility and reliability 

through preset conditions 

and manual controls for 

ultraviolet or time exposures 

• Factory presettings that deliver 120,000 microjoules or 5 minutes of 

exposure; the maximum UV energy setting is 999,900 microjoules per cm2 • Door window that blocks UV radiation, but still allows observation 

of the process 

• Five 8-watt, 254nm ultraviolet tubes for uniform overhead UV illumination 

• Small footprint for benchtop use 


UV Crosslinker, 115V 89131-484 1,586.00 

UV Crosslinker, 230V 89131-486 1,586.00 

UV Hand Lamps 

Ultraviolet lamps have many uses in the micro lab. The 365nm UV lamp can 

be used for water testing, quality control inspection, non-destructive testing, 

and sanitation. The 254nm UV lamp can be used for TLC, E-Coli testing, 

and sterilization. 

Model Wavelength, nm Cat. No. Price 

UV-A 365 89131-488 259.00 

UV-AC 254/365 89131-492 323.00 

UV-AB 302/365 89131-496 333.00 

Note: The lamps use 6-watt tubes and feature a lightweight, rugged 

aluminum design with a scratch-resistant powder paint. 


PolySeed ® 

For BOD5 Analysis 

vs Natural Seed 

Which Seed Should You Use? 

When it comes to deciding between PolySeed or natural seed for BOD5 

testing, the choice is clear. For fast, economical BOD5 analysis with consistent 

results, use PolySeed. Look at the facts and try it for yourself. Contact your 

local VWR Sales Representative to request your free sample today. 

• Pre-measured, 

easy-to-use capsules 

• Non-pathogenic dry 

product rehydrates 

quickly and easily 

• Two year shelf-life 

• Free of nitrifying 

micro-organisms 

• EPA recommended 

• Formulated according to 

The Standard Methods for 

Examination of Water and 

Wastewater 

• Each capsule yields seed 

inoculum for up to 200 

BOD5 tests 

PolySeed Natural Seed 

Convenient Inconvenient 

Safe Safety Hazard 

Consistent Inconsistent 

Known Bacterial Content Unknown Bacterial Content 

Free of Nitrifying Bacteria Possible Nitrifying Bacteria 


PolySeed 66130-430 98.65 

PolySeed-NX CBOD 5 66130-432 139.36 

Polytox ® Rapid Biological Toxicity Screening Test 66130-436 199.98

Masterclave ® 

Series 

Automated Culture 

Media Preparators 

The AES CHEMUNEX Masterclave Series represents the most advanced and 

easy-to-use media preparators available today. With 30 years of experience 

specializing in culture media manufacturing, you can expect the highest 

quality agar and broth preparation. 

Our Masterclaves provide a large magnetic stirrer to ensure homogeneity, and 

feature accurate core temperature monitoring and easy-to-clean 

systems. Sterilization temperatures range from 95 to 125°C. Units also 

feature a sterilization period up to 60 minutes and a dispensing option 

from 25 to 80°C. 

Produce your own high quality media quickly, accurately, and safely! 

• Masterclave 09 capacity 1 to 9L 



Description Capacity, L Cat. No. Price 

Masterclave 09 1 to 9 97059-212 22,808.82 



Culture Media 

and Media Bases 

Microbiology 

For Microbiology and Molecular Biology Applications 

HiMedia ® manufactures 

and supplies high-quality 

products using stringent 

quality parameters. 

Mueller Hinton broth is used 

to determine the susceptibility 

of bacteria to sulphonamides 

by the tube dilution method. 

Luria broth is used for the 

cultivation and maintenance 

of recombinant strains of Escherichia coli. Nutrient Broth is used for 

cultivation of less fastidious organisms, while Lactose broth is used for the 

detection of coliform bacteria in water, foods, and dairy products. Letheen 

broth AOAC is used for determination of bacterial activity of quaternary 

ammonium compounds using Escherichia coli or Staphylococcus aureus. 

Contact your VWR Life Science Specialist to learn more 

about the complete range of HiMedia products available. 


Brain Heart Infusion Broth 95021-488 88.13 

Brain Heart Infusion Broth, HiVeg 61000-752 92.68 

Buffered Peptone Water with NaCl 95020-848 90.24 

Casein Soybean Digest Agar 95021-622 58.62 

Lactose Broth 95020-262 66.88 

Letheen Broth, AOAC 95022-874 122.37 

Luria Broth 95022-016 52.54 

Luria Bertani Agar, Miller 95020-584 86.96 

Luria Bertani HiVeg Agar, Miller 61000-490 86.96 

MacConkey Agar w/ 0.15% Bile Salts, 95020-204 79.57 

CV, and NaCl 

Mueller Hinton Agar 95039-350 78.30 

Mueller Hinton Broth 95039-356 100.97 

Nutrient Agar 95019-996 101.05 

Nutrient Broth 95019-998 80.89 

Nutrient Broth, HiVeg 61000-218 84.93 

Plate Count Agar 95020-238 82.27 

Plate Count Agar, HiVeg 61000-378 86.51 

Potato Dextrose Agar 95020-250 98.03 

Sabouraud Dextrose Agar, HiVeg 61000-326 67.98 

Soybean Casein Digest Medium 95020-030 40.23 

(Tryptone Soya Broth) 

Soybean HiVeg Agar 61000-848 61.57 

* All items listed are 500g; visit vwr.com for recommended applications 


Cell Biology 

Antibodies and Conjugates 

To Power Your Immunoassays 

Boost Sensitivity and Specificity 

KPL has a complete offering of affinity purified secondary antibodies and 

conjugates that provide high signal and low background in immunoassays. 

They recognize a broad range of species labeled with multiple enzyme 

and fluorochrome tags. KPL antibodies enable the development of high 

performance immunoassays, such as ELISA, blotting, and immunochemistry. 

• We make what we sell – KPL controls the entire process from raw 

materials to final product 

• Broad product offering – Antibodies recognize the 

immunoglobulins of over 20 species with seventeen 

labels including a range of DyLight fluorescent tags 

• Products in stock – Available for immediate shipment 

Description 

DyLight 488 

Cat. No. Price 

Anti-Goat IgG (H+L) 95058-496 141.80 

Anti-Human IgG (H+L) 95058-494 131.30 

Anti-Mouse IgG (H+L) HSA 95058-512 131.30 

Anti-Rabbit IgG (H+L) 95058-500 131.30 

Anti-Rat IgG (H+L) 

Horseradish Peroxidase 

95058-504 141.80 

AntiI-Goat IgG (H+L) 95058-778 44.50 

Anti-Human IgG (H+L) 95058-720 60.60 

Anti-Mouse IgG (H+L), HSA 95058-740 57.30 

Anti-Rabbit IgG (H+L) 95058-730 59.60 

Anti-Rat IgG (H+L) 95058-782 44.10 

* All products listed are 1mg 


cellgro ® 

Molecular 

Biology Grade Water 

Mediatech cellgro ® Molecular Biology Grade Water 

is ideal for the preparation of reagents, rinsing 

glassware and plasticware, and other molecular 

biology applications where RNase, DNase, and 

Protease-free water is required. No toxic agents, 

including DEPC, are used in the preparation, 

eliminating possible interference with enzymatic 

reactions. Ideal downstream results require the 

right reagents and you can trust cellgro ® 

Molecular Biology Grade Water for your 

important research needs. 

• Use for preparing reagents and other 

tasks requiring high sterility 

• Produced under cellgro ® 

high industry standards 


Sterile Molecular Grade Water, 1L 45001-044 Pk. 6/ 122.49 

Research Use 

SOC Medium, 10x10mL 45001-099 Ea./ 74.14 

Molecular Reagent 

Electroporation Systems 

BTX ® offers platform 

systems designed to 

provide transfection and 

transformation solutions. 

The ECM 830 square wave 

system efficiently transfects 

mammalian cells, including 

primary cells in vitro using either cuvettes 

or 96-well electroporation. Ideal for transfecting 

delicate tissues in vivo or ex vivo using BTX specialty 

electrodes. The ECM 630 is a powerful exponential 

decay wave system for high efficiency prokaryotic 

transformations in cuvettes alone, or can be expanded 

up to 96-well electroporation for high throughput 

library screening. Both systems can use the BTXpress 

High Performance Electroporation Buffer to increase 

transfection efficiencies and critical cell viability of 

primary cells including difficult to transfect cells. 

A single buffer for all mammalian cell types. 


Electroporation System 58018-004 7,964.14 

Electroporation System w/ Cuvettes 58017-932 6,600.21 

High Solution, 50 Reaction Kit 89130-542 263.99

Cell Biology 

Antibodies 

VWR’s Life Science Portfolio continually 

expands to support your research 

and production needs. 

To learn more, contact us today! 

www.vwr.com 1.800.932.5000 

Cell Counters 

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0611 30M Lit. No. 92872 

32

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AlBioTech . . . . . . . . . . . . . . . . . . . 13 

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HiMedia ® . . . . . . . . . . . . . . . . . . . . 27 

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Idaho Technology Inc. . . . . . . . . . . 6-7 

InterLab ® /Polyseed ® . . . . . . . . . . . . 26 

KAPA Biosystems. . . . . . . . . 20-21, 31 

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Polyplus transfection . . . . . . . . 32, 33 

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Rockland Immunochemicals. . . . . . 19 

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Thermo Scientific . . 16, 19, 24, 31, 32 

VWR . . . . . . . . . . . . . . . . . . . . . 18, 26 

Life Science Magazine vwr.com 800.932.5000 33 

L2K 

FACS analysis 24 hours 

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1.800.932.5000 vwr.com 

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0611 30M Lit. No. 92872 

ATTN. 

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If addressee is no 

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