Surgery and Healing in the Developing World - Dartmouth-Hitchcock
Surgery and Healing in the Developing World - Dartmouth-Hitchcock Surgery and Healing in the Developing World - Dartmouth-Hitchcock
8 60 Surgery and Healing in the Developing World Lab in a suitcase Phase contrast microscope fitted 25-50 blood transfusion bags with anti-coagulant with mirror & light source 500 ml ethanol 10X, 40X, 100X and oil 1000 ml sterile distilled water immersion objectives *20 small test tubes *15 Urine collection bottles *5 glass eye droppers Extra bulbs Leishman stain ˜˜100 ml Oil for viewing Field stain ˜ 50 ml *Hemocytometer Gram stain ˜ 100 ml *100 glass slides Carbon Fuscin stain ˜ 100ml *100 glass cover slips Xylene ˜ 50 ml Syringes (1,5,10,50 ml) HIV & Hapatitis B kit ˜ 50 tests Needles (16,18,20 and 22 G) Widal kit ˜ 50 tests Sterile alcohol swabs Blood typing kit ˜ 50 tests Citrate saline ˜ 100 ml Glucometer *Pipettes 100 b-HCG dipsticks One 40-200 ml 200 Glucose/dextrose sticks One 100-1000 ml 200 Urine dipsticks *50 tips to fit each pipette Extras: Maglite Propane powered Coleman stove 10 candles Battery-powered portable photometer *30 pairs Gloves Enzyme immunoassays, biochemistry and drug Calculator level for serum, plasma and urine *5 ESR tubes Pipette bulb Vertical ESR stand Timer Tweezers 1 box kimwipes 100 ml graduated cylinder *All re-usable if cleaned and dried. Note: Reagents are required if stains are to bemade by the technician power is not available, an alternate must be considered. Solar powered refrigerators are one possibility. Much of the equipment listed in the suitcase has been chosen for its ability to be reused. All glassware, slides, ESR tubes, urine bottle, gloves, pipette tips, etc. can be cleaned, dried and reused. This recycling minimizes the amount of equipment that needs to be transported (Fig. 2). Organizing and Setting Up the Lab A clean, flat surface is necessary when setting up the lab. If a bench top or table is not available, a flat section of ground covered with a clean sheet will suffice. If a room is available, it will optimally include a power and water supply and refrigerator. Large windows covered with fly nets will make the working atmosphere more enjoyable and provide good lighting. In short, a simple room in either a building or dwelling will do quite nicely.
Lab in a Suitcase Figure 1. The phase contrast microscope. Preparing Blood, Plasma and Serum Various procedures will require different sample. If whole anticoagulated blood is needed mix (by inverting gently) 4.5 ml blood with 0.5 ml citrate saline. This will prevent clotting of the sample. If plasma is needed allow the anticoagulated blood sample to sit upright for 2-3 hours. The cells will sediment in the lower half of the tube with plasma on top. If a centrifuge is available, spin the tube at 1000 rpm for 10 min. To isolate serum, obtain a blood sample with no additives. Allow blood to clot for 1 hour and let sit upright for 2-3 hours to allow for separation of cells and serum. As with plasma, serum will be the top layer in the tube. Blood Typing and Transfusion One of the most important and potentially life saving procedures is blood typing and transfusion. It is important to quickly determine the blood type of the parent 61 Figure 2. Re-used glass urine bottles. 8
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60 <strong>Surgery</strong> <strong>and</strong> <strong>Heal<strong>in</strong>g</strong> <strong>in</strong> <strong>the</strong> Develop<strong>in</strong>g <strong>World</strong><br />
Lab <strong>in</strong> a suitcase<br />
Phase contrast microscope fitted 25-50 blood transfusion bags with anti-coagulant<br />
with mirror & light source 500 ml ethanol<br />
10X, 40X, 100X <strong>and</strong> oil 1000 ml sterile distilled water<br />
immersion objectives *20 small test tubes<br />
*15 Ur<strong>in</strong>e collection bottles *5 glass eye droppers<br />
Extra bulbs Leishman sta<strong>in</strong> ˜˜100 ml<br />
Oil for view<strong>in</strong>g Field sta<strong>in</strong> ˜ 50 ml<br />
*Hemocytometer Gram sta<strong>in</strong> ˜ 100 ml<br />
*100 glass slides Carbon Fusc<strong>in</strong> sta<strong>in</strong> ˜ 100ml<br />
*100 glass cover slips Xylene ˜ 50 ml<br />
Syr<strong>in</strong>ges (1,5,10,50 ml) HIV & Hapatitis B kit ˜ 50 tests<br />
Needles (16,18,20 <strong>and</strong> 22 G) Widal kit ˜ 50 tests<br />
Sterile alcohol swabs Blood typ<strong>in</strong>g kit ˜ 50 tests<br />
Citrate sal<strong>in</strong>e ˜ 100 ml Glucometer<br />
*Pipettes 100 b-HCG dipsticks<br />
One 40-200 ml 200 Glucose/dextrose sticks<br />
One 100-1000 ml 200 Ur<strong>in</strong>e dipsticks<br />
*50 tips to fit each pipette Extras:<br />
Maglite Propane powered Coleman stove<br />
10 c<strong>and</strong>les Battery-powered portable photometer<br />
*30 pairs Gloves Enzyme immunoassays, biochemistry <strong>and</strong> drug<br />
Calculator level for serum, plasma <strong>and</strong> ur<strong>in</strong>e<br />
*5 ESR tubes<br />
Pipette bulb<br />
Vertical ESR st<strong>and</strong><br />
Timer<br />
Tweezers<br />
1 box kimwipes<br />
100 ml graduated cyl<strong>in</strong>der<br />
*All re-usable if cleaned <strong>and</strong> dried. Note: Reagents are required if sta<strong>in</strong>s are to<br />
bemade by <strong>the</strong> technician<br />
power is not available, an alternate must be considered. Solar powered refrigerators<br />
are one possibility.<br />
Much of <strong>the</strong> equipment listed <strong>in</strong> <strong>the</strong> suitcase has been chosen for its ability to be<br />
reused. All glassware, slides, ESR tubes, ur<strong>in</strong>e bottle, gloves, pipette tips, etc. can be<br />
cleaned, dried <strong>and</strong> reused. This recycl<strong>in</strong>g m<strong>in</strong>imizes <strong>the</strong> amount of equipment that<br />
needs to be transported (Fig. 2).<br />
Organiz<strong>in</strong>g <strong>and</strong> Sett<strong>in</strong>g Up <strong>the</strong> Lab<br />
A clean, flat surface is necessary when sett<strong>in</strong>g up <strong>the</strong> lab. If a bench top or table<br />
is not available, a flat section of ground covered with a clean sheet will suffice. If a<br />
room is available, it will optimally <strong>in</strong>clude a power <strong>and</strong> water supply <strong>and</strong> refrigerator.<br />
Large w<strong>in</strong>dows covered with fly nets will make <strong>the</strong> work<strong>in</strong>g atmosphere more<br />
enjoyable <strong>and</strong> provide good light<strong>in</strong>g. In short, a simple room <strong>in</strong> ei<strong>the</strong>r a build<strong>in</strong>g or<br />
dwell<strong>in</strong>g will do quite nicely.