20.06.2013 Views

RevisionPharmacognosy2_203 - Faculty of Pharmacy

RevisionPharmacognosy2_203 - Faculty of Pharmacy

RevisionPharmacognosy2_203 - Faculty of Pharmacy

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

Identification Of Unknown Powder<br />

A-Observe the color:<br />

Orange Red Powder Reddish brown Powder Grey Powder<br />

* Fiery pungent taste<br />

* Boil pd with KOH, examine under<br />

microscope Endocarp and seed coat<br />

* Spicy Pungent Taste<br />

* 2dps <strong>of</strong> dil I2 on watch glass, sprinkle<br />

the pd, examine under microscope <br />

Blue particles (Starch Granules)<br />

* Pd + Sudan III Red globules<br />

2012/2013<br />

* Heterogeneous (fluffy & gritty).<br />

* Bitter taste.<br />

* Powder+ Phloroglucin + Conc.HCl<br />

Red color (Broken Lignified Ribs<br />

under microscope)<br />

(Capsicum) (Nutmeg) (Nux vomica)


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

*Wavy Crossed Fibers & simple vittae<br />

(few)<br />

* Powder+ Phloroglucin + Conc.HCl<br />

Red color (lignified wavy crossed<br />

Fibers under microscope)<br />

B- Pd + KOH, Boil (Examine under microscope and Search for vittae)<br />

Vittae present<br />

(Umbelliferous fruit)<br />

2012/2013<br />

Simple vittae + Branched vittae +<br />

* Porous layer.<br />

no hair, no wavy crossed * Non glandular,<br />

fiber& no porous layer. unicellular, conical hair.<br />

* Test for khellin .<br />

Vittae absent<br />

Senna<br />

Linseed<br />

Fenugreek<br />

Black mustard<br />

Psyllium<br />

* Powder+ Phloroglucin +<br />

Conc.HCl Red color<br />

(Reticulate parenchyma<br />

under microscope)<br />

* Spicy aromatic taste. * Bitter taste. * Sweet aromatic taste. Sweet aromatic taste.<br />

(Coriander) (Ammi visnaga) (Fennel) (Anise)


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

* Crossed<br />

Fibers<br />

* Non<br />

glandular,<br />

unicellular hair<br />

(few)<br />

Senna<br />

* Mat-like structure<br />

* Pigment layer<br />

(Linseed)<br />

Vittae absent<br />

* Polygonal Network<br />

Reticulation<br />

(Black<br />

Mustard)<br />

* Palisade-like<br />

Epidermal cells<br />

* Basket- like<br />

hypodermal cells<br />

( Foenugreek)<br />

2012/2013<br />

* Mucilaginous touch<br />

* Epidermal cells <strong>of</strong><br />

ventral side<br />

(Psyllium)


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

Scheme for Identification <strong>of</strong> Vegetable Powder<br />

I. Physical characters<br />

1) Condition: powder<br />

2) Colour:<br />

Pale powder:<br />

Psyllium (brownish buff)<br />

Nux vomica (Grey)<br />

Foenugreek (yellowish green)<br />

Dark powder:<br />

Linseed & Black mustard (yellowish brown)<br />

Nutmeg (reddish brown)<br />

Capsicum (Orange red)<br />

Senna (dark greenish brown)<br />

Fennel, Anise, Ammi visnaga &Coriander (greenish brown)<br />

3) Odour:<br />

Aromatic: Nutmeg, Fennel, Anise & Coriander.<br />

Sternutatory: Capsicum.<br />

Spicy characteristic: Foenugreek.<br />

Pungent: Black mustard.<br />

Odourless: Psyllium & Senna.<br />

Characteristic: rest <strong>of</strong> the powders.<br />

4) Taste:<br />

Fiery pungent: Capsicum.<br />

Aromatic spicy: Nutmeg & Coriander.<br />

Slightly bitter, pungent & mucilaginous: Black mustard.<br />

Bitter: Nux vomica, Ammi visnaga .<br />

Bitter mucilaginous: Foenugreek & Senna.<br />

Oily mucilaginous: Linseed.<br />

Strongly mucilaginous: Psyllium.<br />

Sweet aromatic: Fennel & Anise.


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

II. Preliminary chemical tests<br />

1) Press between two filter papers:<br />

Oily stain (oil): all seeds and fruits except Psyllium, Senna .<br />

2) Press between two moist fingers:<br />

Mucilaginous touch ( mucilage): Psyllium, Foenugreek, Black mustard,<br />

Linseed & Senna.<br />

No mucilaginous touch: rest <strong>of</strong> the powders.<br />

3) Effect <strong>of</strong> iodine (T.S.):<br />

Blue stain (starch): Nutmeg.<br />

No blue stain: rest <strong>of</strong> the powders.<br />

4) Effect <strong>of</strong> potassium hydroxide (T.S):<br />

Yellow colour (flavonoids): Foenugreek.<br />

Reddish brown colour on hot (sennosides): Senna.<br />

5) Effect <strong>of</strong> ferric chloride ( T.S.):<br />

Greenish colour (tannins): Nutmeg.<br />

6) Effect <strong>of</strong> 80% sulphuric acid:<br />

No red colour: all seeds & fruits.<br />

III. Microscopical chemical tests<br />

1) Effect <strong>of</strong> iodine (T.S.):<br />

Blue stain (starch): Nutmeg.<br />

Brown stain (proteins): all seeds & fruits.<br />

2) Effect <strong>of</strong> Sudan III:<br />

Red stain (due to oil): all seeds and fruits.<br />

3) Effect <strong>of</strong> ruthenium red :<br />

Red stain (acidic mucilage): Psyllium, Black mustard, Linseed, & Senna.<br />

No red stain: rest <strong>of</strong> the powders.<br />

4) Effect <strong>of</strong> picric acid:<br />

Yellow stain (proteins): all seeds & fruits.<br />

5) Effect <strong>of</strong> potassium hydroxide solution:<br />

Reddish brown colour on hot (sennosides): Senna.<br />

6) Effect <strong>of</strong> ferric chloride:<br />

Greenish colour (tannins): Nutmeg.<br />

7) Effect <strong>of</strong> 80% sulphuric acid:<br />

No red colour: all seeds & fruits.


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

IV. Microscopical examination Includes:<br />

Labeled Drawings<br />

Description<br />

V. Conclusion<br />

English name: …………………..<br />

Origin: …………………………<br />

VI. Special chemical tests<br />

Linseed: Guignard paper test.<br />

Foenugreek: Froth test.<br />

Psyllium: Ruthenium red.<br />

Black mustard, Nutmeg, Fennel, Anise & Coriander: Sudan III.<br />

Ammi visanga : Test for khellin (powder decoction + KOH pellets).<br />

Capsicum: test for capsaicin.<br />

Senna: Modified Borntrager`s test.<br />

Nux vomica: Nitric acid.<br />

Wish you Good Luck<br />

Course coordinator<br />

Ass. Pr<strong>of</strong>. Dr. Maha M. Salama<br />

And<br />

Staff Members <strong>of</strong> Pharmacognosy-2 (<strong>203</strong>)


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

Hypodermis<br />

sclerenchyma<br />

Hyaline<br />

layer<br />

Endosperm<br />

T.S. in linseed<br />

T.S. In Foenugreek<br />

Thick<br />

cuticle<br />

Epidermis<br />

Pigment layer<br />

Epiderms<br />

(Palisade-like cells)<br />

Hypodermis<br />

(Basket-like cells)


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

T.S. in Capsicum seed<br />

Vitte<br />

V.B.<br />

T.S. in Fennel<br />

Orthospermous<br />

Endosperm<br />

Epicarp<br />

Hyaline layer<br />

Endocarp<br />

Testa


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

Branched Vittae<br />

1ry ridge<br />

Vascular bundle<br />

Vittae Orthospermous endosperm<br />

More prominent<br />

2ry ridge<br />

T.S. in Anise<br />

Less prominent<br />

1ry ridges<br />

Vittae Coelospermous endosperm<br />

T.S. in Coriander


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

T.S. in Ammi visnaga<br />

Orthospermous<br />

endosperm<br />

1ry ridge is more prominent than 2ry ridge<br />

T.S. in Ammi majus<br />

Orthospermous<br />

endosperm<br />

1ry ridge & 2ry ridge are equally prominent<br />

lacuna<br />

Fan like structure<br />

IML <strong>of</strong> mesocarp<br />

Crown shaped V.B.<br />

Papillosed Epicarp<br />

Fan-like structure


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

T.S. in capsicum pericarp<br />

(family solanaceae)<br />

Islets <strong>of</strong> scleriedes<br />

IMLM<br />

(Giant cell)<br />

Epicarp<br />

Wish you Good Luck<br />

Course coordinator<br />

Ass. Pr<strong>of</strong>. Dr. Maha M. Salama<br />

And<br />

Staff Members <strong>of</strong> Pharmacognosy-2 (<strong>203</strong>)


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

Spots


Cairo University Pharmacognosy-2 (<strong>203</strong>)<br />

<strong>Faculty</strong> <strong>of</strong> <strong>Pharmacy</strong><br />

Pharmacognosy Department<br />

Wish you Good Luck<br />

Course coordinator<br />

Ass. Pr<strong>of</strong>. Dr. Maha M. Salama<br />

And<br />

Staff Members <strong>of</strong> Pharmacognosy-2 (<strong>203</strong>)

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!