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OBEN GAS DEVELOPMENT PROJECT

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Appendixes<br />

acidified to a pH of 1.5 for heavy metal analysis and the others kept at 4 o C) and then transported to the<br />

laboratory for analyses.<br />

Water temperature was measured in situ using Radiometer, while transparency was determined with a<br />

Turbidimeter. Sample for dissolved oxygen (DO) was fixed in the field using 1.0 ml each of Winkler’s Solutions<br />

A and B (APHA, 1989).<br />

Samples for BOD5 determination were collected in black 250ml reagent bottles and taken to the laboratory for a<br />

five-day incubation, fixing and analysis.<br />

Hydrogen-ion concentration (pH) , conductivity, turbidity, salinity, and total dissolved solids were measured in<br />

situ using Radiometer (portable digital meters).<br />

Dissolved oxygen and BOD5 samples were analysed in the laboratory using the Winkler’s titrimetric method.<br />

i. Phytoplankton and Zooplankton<br />

Composite samples were taken quantitatively by filtering 100 litres of water through 55µm Hydrobios plankton<br />

net. All samples were preserved in 4% buffered formaldehyde in labeled polyethylene bottles and taken to the<br />

laboratory for analysis.<br />

ii. Benthic fauna<br />

The light dredge or Ekman grab was used in sampling benthic fauna. The grab samples were sieved at the<br />

station using 500µm sieve and preserved using 4% buffered formaldehyde. The labeled samples were taken to<br />

the laboratory for analysis.<br />

Hydrogeology and hydrology<br />

A total of five (5) environmental boreholes were investigated but samples were collected from three existing<br />

boreholes for in-situ measurements. The water bearing rocks (aquifer) are generally shallow and the static<br />

water levels in the area range between 13.21m-14.53m. The subsurface materials consist of topsoils which are<br />

brownish in colour and predominantly silty sands followed by sands of different grain sizes and shapes.<br />

Groundwater Sampling<br />

The modified HACH groundwater sampler was used to collect samples after flushing the holes. In- situ<br />

measurements for pH, temperature, conductivity, salinity, TDS and Turbidity were conducted for these<br />

samples.<br />

The insitu measurements were taken with the aid of Turbidimeter and Radiometer respectively for Turbidity,<br />

TDS, DO, pH ,Salinity and Electrical Conductivity. Here data were collected by filling water sample into a<br />

sample cell and the equipment’s nob was press to boot on. The sample cell with water sample was inserted<br />

into the equipment and the READ command prompted for data analysis.<br />

Groundwater Flow Direction<br />

The direction of groundwater flow in the project was determined using the three existing environmental<br />

boreholes. The boreholes were located in a triangular manner and the static level was measured in each with<br />

Fisher Model WLT electric water level indicator.<br />

A-7

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