Metabolomics - CERM

Celiac Disease Metabolomics
Experimental scheme:
• Study subjects: 34
• Control subjects: 34
• Samples: Serum and Urine
NMR spectra acquired:
• 1D Noesy (standard 1D 1H spectra) for serum and urine samples
• CPMG: to remove signals due to macromolecules (on serum samples)
• @ a Bruker 600 MHz
Statistical
Analysis
Projection to Latent Structures (PLS) to reduce data dimension Optimal number of
components obtained by minimizing the Cross-Validated (CV) error
Canonical Analysis (CA) to obtain two well separated clusters
Support Vector Machines (SVM) for classification