Metabolomics - CERM

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Challenges when analyzing metabolomes Metabolomes extend over 7 to 9 order of magnitudes in concentration (picomoles to millimoles) Currently not possible to analyze all metabolites in a single analysis Several analytical strategies (MS, NMR in combination with chromatographic separations, whole cell analysis) Requires high throughput
Advantages: Use of NMR in Non-destructive, non-biased Easily quantifiable Requires little or no separation metabolomics studies Permits identification of novel compounds Does not require chemical derivatization Particularly amenable to cmpds less tractable to GC-MS or LC-MS (sugars, amines, volatile ketones, & relatively non-reactive compounds) Ref. Trends in analytical chemistry (2008) Vol 27, pp.228-237
Page 1 and 2: Metabolomics Leonardo Tenori FiorGe
Page 3 and 4: “La metabolomica è l’ultima na
Page 5 and 6: Metabolomica: la nuova frontiera
Page 7 and 8: Metabonomics “…measurement of t
Page 9 and 10: Metabolomica: alcuni obiettivi Valu
Page 11 and 12: Metabolomica: alcuni obiettivi Scop
Page 13 and 14: The metabolome consists of what? Sm
Page 15 and 16: C om p ound class N u m b er C om p
Page 17 and 18: Why 1 µM? Equals ~200 ng/mL Limit
Page 19 and 20: Medical Metabolomics Generate metab
Page 21 and 22: Traditional Metabolite Analysis HPL
Page 23 and 24: What’s the Difference Between Met
Page 25 and 26: Advantages Measure multiple (10’s
Page 27 and 28: 2 Routes to Metabolomics Two approa
Page 29: Benefits of analyzing the metabolom
Page 33 and 34: Raccolta e stoccaggio dei campioni
Page 35 and 36: NMR A typical 950-MHz H NMR spectru
Page 37 and 38: Profilo 1 H NMR di urina umana
Page 39 and 40: Profilo di siero umano Proteins + L
Page 41 and 42: Data analysis - approach Classify N
Page 43: PCA Unsupervised Multivariate analy
Page 51 and 52: PCA Loadings Plot • Loadings plot
Page 53 and 54: SIMCA Supervised learning method ba
Page 55 and 56: OPLS OPLS method is a recent modifi
Page 57 and 58: Targeted profiling Targeted metabol
Page 59 and 60: Databases
Page 61 and 62: Effetto della dieta Trimetilammina
Page 63 and 64: Effetto delle patologie Campioni di
Page 65 and 66: METabolomic REFerence The METREF pr
Page 67 and 68: Ind 1 Ind 2 METabolomic REFerence G
Page 69 and 70: METabolomic REFerence Dendrogram of
Page 71 and 72: METabolomic REFerence An individual
Page 73 and 74: METabolomic REFerence 2 Experimenta
Page 75 and 76: METabolomic REFerence 2 2005 collec
Page 77 and 78: Un “salto” metabolico Evoluzion
Page 79 and 80: Celiac Disease Metabolomics Experim
Page 81 and 82:
Celiac Disease Metabolomics Note: b
Page 83 and 84:
Celiac Disease Metabolomics Celiac
Page 85 and 86:
Celiac Disease Metabolomics Cluster
Page 87 and 88:
Potential celiac disease Subjects:
Page 89 and 90:
Celiachia Potenziale Celiaci - Sani
Page 91 and 92:
ACIDO LINOLENICO REGIONE DEI METILI
Page 93 and 94:
PRODOTTI DI OSSIDAZIONE QUASI ASSEN
Page 95 and 96:
CARATTERIZZAZIONE GEOGRAFICA ( 1 H)
Page 97 and 98:
Metabolomica del latte Recente inte
Page 99 and 100:
Spettro 1 H NMR di marche diverse N
Page 101 and 102:
Formato Spettro 1 H NMR di latte di
Page 103 and 104:
formato Formato acetato e piruvato
Page 105:
Disponibilità di Tesi in METABOLOM
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Metabolomics - CERM

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