Metabolomics - CERM
Metabolomics - CERM Metabolomics - CERM
Systems Biology and the rise of the “-omics” Omics technologies such as genomics and high-throughput DNA sequencing were introduced in parallel to the Human Genome Project since 1990s. According to one etymological analysis, the suffix 'ome' is derived from the Sanskrit OM ("completeness and fullness") (Lederberg and McCray, 2001). Omics technologies and various neologisms that define their application contexts, however, are more than a simple play on words. They substantially transformed both the throughput and the design of scientific experiments. The omics technologies allow the generation of copious amounts of data at multiple levels of biology from gene sequence and expression to protein and metabolite patterns underlying variability in cellular networks and function of whole organ systems (Nicholson and Lindon, 2008; Wilke et al., 2008) Genomics Study of genes Epigenomics The study of the complete set of epigenetic (DNA methylation) modifications on the genetic material of a cell, known as the epigenome Transcriptomics All the mRNA in a cell/tissue/organism Proteomics All the proteins in a cell/tissue/organism Metallomics comprehensive analysis of the entirety of metal and metalloid species within a cell or tissue type Metabonomics/Metabolomics All the metabolites in a cell/tissue/organism
“La metabolomica è l’ultima nata tra le scienze omiche e ha lo scopo di studiare il metaboloma, che è l’insieme di tutti i metaboliti contenuti in un fluido biologico (o cellula o tessuto)”. Cos’è la Metabolomica Punti di forza: forza Genomics: the only -omics which is not context dependent Metabolomics: strong environmental influence L’insieme dei metaboliti rappresenta l’espressione l’espressione amplificata amplificata del genoma I metaboliti sono caratterizzati da elevata stabilità. stabilità Ciò permette una elevata precisione e riproducibilità delle misure L’analisi metabolomica scatta “un’istantanea un’istantanea” dello stato di salute o malattia di un soggetto
- Page 1: Metabolomics Leonardo Tenori FiorGe
- Page 5 and 6: Metabolomica: la nuova frontiera
- Page 7 and 8: Metabonomics “…measurement of t
- Page 9 and 10: Metabolomica: alcuni obiettivi Valu
- Page 11 and 12: Metabolomica: alcuni obiettivi Scop
- Page 13 and 14: The metabolome consists of what? Sm
- Page 15 and 16: C om p ound class N u m b er C om p
- Page 17 and 18: Why 1 µM? Equals ~200 ng/mL Limit
- Page 19 and 20: Medical Metabolomics Generate metab
- Page 21 and 22: Traditional Metabolite Analysis HPL
- Page 23 and 24: What’s the Difference Between Met
- Page 25 and 26: Advantages Measure multiple (10’s
- Page 27 and 28: 2 Routes to Metabolomics Two approa
- Page 29 and 30: Benefits of analyzing the metabolom
- Page 31 and 32: Advantages: Use of NMR in Non-destr
- Page 33 and 34: Raccolta e stoccaggio dei campioni
- Page 35 and 36: NMR A typical 950-MHz H NMR spectru
- Page 37 and 38: Profilo 1 H NMR di urina umana
- Page 39 and 40: Profilo di siero umano Proteins + L
- Page 41 and 42: Data analysis - approach Classify N
- Page 43: PCA Unsupervised Multivariate analy
- Page 51 and 52: PCA Loadings Plot • Loadings plot
“La metabolomica è l’ultima nata tra le scienze omiche e ha lo scopo di studiare il<br />
metaboloma, che è l’insieme di tutti i metaboliti contenuti in un fluido biologico (o cellula<br />
o tessuto)”.<br />
Cos’è la Metabolomica<br />
Punti di forza: forza<br />
Genomics: the only -omics which is not context dependent<br />
<strong>Metabolomics</strong>: strong environmental influence<br />
L’insieme dei metaboliti rappresenta<br />
l’espressione<br />
l’espressione amplificata amplificata del<br />
genoma<br />
I metaboliti sono caratterizzati da<br />
elevata stabilità. stabilità Ciò permette<br />
una elevata precisione e<br />
riproducibilità delle misure<br />
L’analisi metabolomica scatta<br />
“un’istantanea<br />
un’istantanea” dello stato di<br />
salute o malattia di un soggetto
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