Metabolomics - CERM
Metabolomics - CERM Metabolomics - CERM
Problems with Traditional Methods Requires separation followed by identification (coupled methodology) Requires optimization of separation conditions each time Often requires multiple separations Slow (up to 72 hours per sample) Manually intensive (constant supervision, high skill, tedious)
What’s the Difference Between Metabolomics and Traditional Clinical Chemistry? Throughput (more metabolites, greater accuracy, higher speed)
- Page 1 and 2: Metabolomics Leonardo Tenori FiorGe
- Page 3 and 4: “La metabolomica è l’ultima na
- Page 5 and 6: Metabolomica: la nuova frontiera
- Page 7 and 8: Metabonomics “…measurement of t
- Page 9 and 10: Metabolomica: alcuni obiettivi Valu
- Page 11 and 12: Metabolomica: alcuni obiettivi Scop
- Page 13 and 14: The metabolome consists of what? Sm
- Page 15 and 16: C om p ound class N u m b er C om p
- Page 17 and 18: Why 1 µM? Equals ~200 ng/mL Limit
- Page 19 and 20: Medical Metabolomics Generate metab
- Page 21: Traditional Metabolite Analysis HPL
- Page 25 and 26: Advantages Measure multiple (10’s
- Page 27 and 28: 2 Routes to Metabolomics Two approa
- Page 29 and 30: Benefits of analyzing the metabolom
- Page 31 and 32: Advantages: Use of NMR in Non-destr
- Page 33 and 34: Raccolta e stoccaggio dei campioni
- Page 35 and 36: NMR A typical 950-MHz H NMR spectru
- Page 37 and 38: Profilo 1 H NMR di urina umana
- Page 39 and 40: Profilo di siero umano Proteins + L
- Page 41 and 42: Data analysis - approach Classify N
- Page 43: PCA Unsupervised Multivariate analy
- Page 51 and 52: PCA Loadings Plot • Loadings plot
- Page 53 and 54: SIMCA Supervised learning method ba
- Page 55 and 56: OPLS OPLS method is a recent modifi
- Page 57 and 58: Targeted profiling Targeted metabol
- Page 59 and 60: Databases
- Page 61 and 62: Effetto della dieta Trimetilammina
- Page 63 and 64: Effetto delle patologie Campioni di
- Page 65 and 66: METabolomic REFerence The METREF pr
- Page 67 and 68: Ind 1 Ind 2 METabolomic REFerence G
- Page 69 and 70: METabolomic REFerence Dendrogram of
- Page 71 and 72: METabolomic REFerence An individual
Problems with<br />
Traditional Methods<br />
Requires separation followed by<br />
identification (coupled methodology)<br />
Requires optimization of separation<br />
conditions each time<br />
Often requires multiple separations<br />
Slow (up to 72 hours per sample)<br />
Manually intensive (constant supervision,<br />
high skill, tedious)
Change language