Preprint volume - SIBM
Preprint volume - SIBM
Preprint volume - SIBM
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Pre-print Volume – Posters<br />
AQUACULTURE COMMITEE<br />
ATPase, 1:100. PCDDs, PCDFs, DL-PCBs and NDL-PCBs were measured in muscle<br />
with high resolution gas chromatography coupled with high-resolution spectrometry.<br />
GST and CAT activities were determined in liver (Ferreira et al. 2010).<br />
Results – The sum of the six NDL-PCB indicators (∑6NDL-PCBs), was 6.28±1.67 and<br />
2.64±2.48, ng/g whole weight (Ww) in Fsb and Wsb, respectively. The TEQ (2006) DL-<br />
PCBs (pg/g -1 Ww) in Fsb and Wsb was 0.28±0.22 and 0.68±0.47, respectively. The<br />
∑TEQ-PCDD/F (pg/g Ww) in Fsb and Wsb were 0.17±0.13. Mean CF (1.5±0.03) and HSI<br />
(2.38±0.46) values were higher in Fsb than in Wsb (0.98±0.03; 0.97±0.05). All tissues<br />
were well structured, even if weak increase of the connective stroma texture in the<br />
testis, moderate increase of lipid vesicles in the hepatocytes and areas with reduction of<br />
respiratory lamellae in the gills were described in Fsb. Catalase activity measured in<br />
Fsb was 93 μmol/min/mg protein, higher than in Wsb. No significant differences were<br />
described for GST among Fsb and Wsb (77.64 and 68.35nmol/min/mg protein,<br />
respectively).<br />
Conclusions – The PCB concentrations in Fsb were in the range of those detected in<br />
other investigations in the Mediterranean Sea. TEQ (2006) DL-PCBs, values were<br />
significantly lower than the maximum levels allowed by the European Commission<br />
(EC, 2006) and lower than those measured in Carubelli et al. (2007), whereas the<br />
∑6NDL-PCBs were comparable. ∑TEQ-PCDD/F were of the same order than that measured in<br />
Çakıroğulları et al. (2010). CF and HSI were higher in Fsb than in Wsb as expected,<br />
while no significant differences were described for morphological parameters in Fsb in<br />
off-shore cages. GST activity in Fsb was not significantly different from that measured<br />
in Wsb but significantly higher than in other farmed sea bass (Ferreira et al., 2010).<br />
CAT activity in Fsb was higher than that measured in other farmed sea bass (Ferreira et<br />
al., 2010), denoting an efficient antioxidant answer. All together, these data confirm<br />
that currents and water deep are the key factors that increase the quality of the rearing<br />
environment, facilitating water exchange inside the cages, reducing the organic matter<br />
sedimentation under the farm and avoiding self-pollution.<br />
References<br />
ÇAKIROĞULLARI G.C., KILIÇ D., UÇAR Y. (2010) - Levels of polychlorinated dibenzo-pdioxins,<br />
dibenzo-p-furans and polychlorinated biphenyls in farmed sea bass (Dicentrarchus<br />
labrax) and sea bream (Sparus aurata) from Turkey. Food Control,<br />
doi:10.1016/j.foodcont.2010.02.010<br />
CARUBELLI G., FANELLI R., MARIANI G., NICHETTI S., CROSA G., CALAMARI D.,<br />
FATTORE E. (2007) – PCB contamination in farmed and wild sea bass (Dicentrarchus labrax<br />
L.) from a coastal wetland area in central Italy. Chemosph., 68: 1630-1635.<br />
EC (2006) – Commission Regualtion (EC) No 199/2006 of 3 February 2006.<br />
FERREIRA M., CAETANO M., ANTUNES P., GIL O., COSTA J., GIL D., BANDARRA N.,<br />
POUSAO-FERREIRA P., VALE C., REIS-HNRIQUES M. A. (2010) – Assessment of<br />
contaminants and biomarkers of exposure in wild and farmed sea bass. Ecotoxicol. Environ. Saf,<br />
doi:10.1016/j.ecoenv.2010.01.019<br />
TOFT G., EDWARDS T.M., AATRUP E., GUILLETTE L.J. (2003) – Disturbed sexual<br />
characteristics in male mosquitofish (Gambusia holbrooki) from a lake contaminated with<br />
endocrine disruptors. Environ. Health. Perspect., 111: 695-701.<br />
VAN DER OOST R., BEYER J., VERMEULEN N. P. E. (2003) – Fish bioaccumulation and<br />
biomarkers in environmental risk assessment: a review. Envir. Toxicol. Pharmacol., 13: 57-149.<br />
41 st S.I.B.M. CONGRESS Rapallo (GE), 7-11 June 2010<br />
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