Preprint volume - SIBM
Preprint volume - SIBM
Preprint volume - SIBM
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Pre-print Volume – Posters<br />
AQUACULTURE COMMITEE<br />
I. TRAVERSI, A. MANDICH, A. CEVASCO, E. FATTORE * , C. GENEROSO * , A. MASSARI<br />
Dipartimento di Biologia, Università di Genova, viale Benedetto XV, 5 - 16132 Genova, Italia.<br />
traversiilaria@gmail.com<br />
* Istituto Mario Negri, Via Giuseppe La Masa, 19 - 20156 Milano, Italia.<br />
MORPHOLOGICAL AND BIOCHEMICAL RESPONSES<br />
TO ENVIRONMENTAL CONTAMINANTS IN FARMED<br />
AND WILD EUROPEAN SEABASS (DICENTRARCHUS LABRAX)<br />
RISPOSTE MORFOLOGICHE E BIOCHIMICHE A CONTAMINANTI<br />
AMBIENTALI<br />
IN SPIGOLA (DICENTRARCHUS LABRAX) ALLEVATA E SELVATICA<br />
Abstract – The presence of environmental pollutants in both wild and cultured fish is cause of concern. The<br />
European sea bass (Dicentrarchus labrax L., Teleostei, Moronidae) represents one of the most diffused<br />
marine species cultured in the Mediterranean areas. Aim of this study was to investigate the relationships<br />
of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDDs and PCDFs), dioxin-like and non dioxinlike<br />
polychlorinated biphenyl (DL and NDL-PCBs) levels in the muscle of wild and cultured sea bass with<br />
morphological and enzymatic activities as biochemical biomarkers of exposure. In farmed fish, levels of<br />
contaminants were lower than those detected in other studies. The low level of contamination was<br />
confirmed both by enzymatic activities and by gonad, liver and gill morphology showing absence of chronic<br />
alterations.<br />
Key-words: environmental pollutants, biomarker, seabass, aquaculture.<br />
Introduction – Polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs)<br />
and polychlorinated biphenyls (PCBs) are persistent contaminants widespread in the<br />
environment and animal tissues (Toft et al., 2003). The integrated use of chemical<br />
analysis and morphological/biochemical markers of exposure makes it possible to<br />
study the impact of anthropogenic contaminants and to compare the effects on wild and<br />
reared fish. The aim of this study was to investigate the relationships between PCDDs,<br />
PCDFs, DL-PCBs, NDL-PCBs levels, morphological endpoints in gonads, liver and<br />
gills and glutathione S-tranferase (GST) and Catalase (CAT) activities as biomarkers of<br />
exposure (Van der Oost et al., 2003) in wild (Wsb) and farmed (Fsb) European sea<br />
bass, Dicentrarchus labrax, selected as bioindicator species<br />
Materials and methods – Farmed D. labrax (n=20; 327.13±57.62 g) were collected,<br />
from an off-shore fish farm located in the Ligurian sea, while wild specimens (n=8,<br />
714.7±58.3), were captured nearby the Cinque Terre National Park,both in February-<br />
March 2009. Fish were measured and weighted and the condition factor (CF: body<br />
weight (g) ×100/length 3 (cm)) and the hepatosomatic index (HSI; liver weight (in<br />
g)/body weight (g) ×100) were calculated. Gonad, liver and gill fragments were<br />
Bouin’s fixed and stained with haematoxylin-eosin. Two series of 4 μm thick sections<br />
were analyzed for presence/absence of alteration of connective stroma, granulocyte and<br />
melanomacrophage centres (MMCs) infiltration, haemorrhages, gamete atresia and<br />
oocytes in testis, for gonads; granulocyte and MMCs infiltration, and lipid deposit in<br />
hepatocytes, for liver; alteration of primary lamellae and respiratory lamellae,<br />
haemorrhages, and relative number of chloride cells and mucous cells, for gills.<br />
Chloride cells were identified by using the mouse raised monoclonal ab-Na, K-<br />
41 st S.I.B.M. CONGRESS Rapallo (GE), 7-11 June 2010<br />
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