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Sorghum Diseases in India

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4. Us<strong>in</strong>g male sterile and fertile cultivars, determ<strong>in</strong>e<br />

the precise period of susceptibility of<br />

fertilized gynoecia of pathogens from Africa,<br />

<strong>India</strong>, and Thailand <strong>in</strong> several environments.<br />

5. Survey farmers' practices, particularly with<br />

respect to knowledge of ergot contam<strong>in</strong>ation<br />

(sclerotia and/or sexual conidia) of seed and<br />

to postharvest land management.<br />

6. Standardize resistance-screen<strong>in</strong>g techniques<br />

and evaluation of resistant genotypes, notably<br />

<strong>in</strong>clud<strong>in</strong>g Ethiopian sorghum. Resistance<br />

<strong>in</strong> this genotype was claimed <strong>in</strong> the 1978<br />

Workshop at Hyderabad.<br />

7. Determ<strong>in</strong>e role of sclerotia <strong>in</strong> epidemiology of<br />

ergot disease.<br />

8. Assess losses to ergot <strong>in</strong> sorghum.<br />

Pearl Millet Ergot<br />

1. Determ<strong>in</strong>e the importance, to farmers, of<br />

pearl millet ergot with respect to gra<strong>in</strong> yield<br />

losses and to toxicity of the sclerotia to humans,<br />

livestock, and poultry.<br />

2. Fully test (as alternatives to resource-consum<strong>in</strong>g<br />

ergot-resistant breed<strong>in</strong>g programs for<br />

pearl millet) other possibilities, <strong>in</strong>clud<strong>in</strong>g use<br />

of exist<strong>in</strong>g ergot-resistant populations as f<strong>in</strong>ished<br />

varieties, pollen management (pollendonor<br />

variety <strong>in</strong>tersown with a conventional<br />

hybrid) at the farmers' level, and hybrids not<br />

based on sterile cytoplasm (e.g., topcross<br />

hybrids).<br />

3. Study (where resources are available) the biology/epidemiology<br />

of pearl millet ergot and<br />

resistance mechanisms, <strong>in</strong>clud<strong>in</strong>g postpoll<strong>in</strong>ation<br />

stigmatic constriction or similar<br />

ag<strong>in</strong>g phenomena.<br />

4. Investigate doubled haploids as a means of<br />

fix<strong>in</strong>g short protogeny (ergot resistance) <strong>in</strong><br />

pearl millet l<strong>in</strong>es.<br />

5. Encourage <strong>in</strong>stitutions funded for basic research<br />

to study the mechanism of postpoll<strong>in</strong>ation<br />

stigmatic constriction, which<br />

excludes pathogenic parasitization of the<br />

ovary via the stigma.<br />

Bacterial <strong>Diseases</strong><br />

1, Document <strong>in</strong>cidence and importance of bacterial<br />

diseases by publish<strong>in</strong>g <strong>in</strong> appropriate<br />

journals, newsletters, and data bases.<br />

336<br />

2. Determ<strong>in</strong>e, where possible, sources of resistance<br />

from hybrids, cultivars, or landraces.<br />

3. Determ<strong>in</strong>e effect of plant-pathogenic bacteria<br />

on stalk rot.<br />

4. Develop techniques such as semiselective<br />

media or serological procedures for accurate<br />

identification of bacterial causal agents.<br />

5. Determ<strong>in</strong>e role of seed transmission <strong>in</strong> dissem<strong>in</strong>ation<br />

of bacterial diseases.<br />

6. Identify portals of entry of plant pathogenic<br />

bacteria <strong>in</strong>to plants through study of hydathodes,<br />

stomates, and other plant structures.<br />

Study effects of wounds.<br />

7. Publish and distribute illustrated handbooks<br />

on bacterial diseases to national scientists<br />

work<strong>in</strong>g with sorghum and pearl millet <strong>in</strong> the<br />

field.<br />

Virus <strong>Diseases</strong><br />

1. Cont<strong>in</strong>ue survey and identification programs<br />

for sorghum viruses and virus diseases.<br />

2. Study crop losses to virus diseases.<br />

3. Study epidemiology host/vector/virus <strong>in</strong>teractions.<br />

4. Standardize <strong>in</strong>oculation techniques and virus-severity<br />

rat<strong>in</strong>g scales.<br />

5. Develop short- and long-term strategies for<br />

manag<strong>in</strong>g sorghum virus diseases.<br />

6. Study genetics of resistance: its mechanisms<br />

and the role of <strong>in</strong>heritance.<br />

7. Develop an <strong>in</strong>dex of 'resistance' as a criterion<br />

for selection.<br />

8. Standardize and publish techniques for<br />

research workers <strong>in</strong> sorghum and millet<br />

virology:<br />

a. ELISA<br />

b. electron microscopy<br />

c. <strong>in</strong>oculation<br />

d. rat<strong>in</strong>g scales<br />

e. screen<strong>in</strong>g<br />

9. Collaboration and tra<strong>in</strong><strong>in</strong>g<br />

a. Provide tra<strong>in</strong><strong>in</strong>g opportunities for plant<br />

virologists <strong>in</strong> develop<strong>in</strong>g nations.<br />

b. International cooperation <strong>in</strong> serology and<br />

electron microscopy for identification of<br />

sorghum and millet virus diseases.<br />

c. Expand tra<strong>in</strong><strong>in</strong>g <strong>in</strong> molecular aspects of virus<br />

identification.<br />

d. Organize and conduct an <strong>in</strong>ternational<br />

workshop on sorghum and millet viruses<br />

and virus diseases.

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