Sorghum Diseases in India

Table 4. Reaction of six Xanthomonas campestris pv holcicola isolates on eight sorghum lines. 1 .
Reaction to Xanthomonas campestris pv holcicola isolates from
Line Nebraska Kansas Texas Mexico Lesotho Australia
BTx 623 R 2
S 3
S S S S
BTx 630 R R R R R R
B 8106 R R S S R R
QL 3 R R R R R R
TAM 428 R S S S S S
Tx 434 R S S S s s
84 C-7730 R s S s s s
85 L-21905 R s s s s s
1. Source: Leach et al., unpublished.
2. R = resistant reaction.
3. S = susceptibility.
veloped for recovering X. campestris pv phaseoli
from common blight-infested bean debris. Reducing
the concentration of gentamycin from 2 mg
L -1 to 1 mg L -1 provided an excellent medium for
X. campestris pv holcicola. The medium consisted
of 0.8 g K2HPO4, 0.6 g KH2PO4, 0.7 g yeast extract,
8 g soluble potato starch, 10 g potassium
bromide, 1 g glucose, and 15 g agar in 1 L of
distilled water. After autoclaving and after the
medium cooled to 48°C, add 1 mL of Daconil 2787
(chlorothalonil ® ; to prepare stock suspension,
add 1.2 mL of Daconil ® 2787 to 38.8 mL of H2O);
10 mL of cephalexin (for stock Solution, add 1.0 g
cephal-exinmonohydrate to 500 ml H2O); 10 mL
of kasugamycin [stock solution, add 1.0 g kasugamycin
hemisulfate to 500 µl H2O), and 5 mL
of gentamycin sulfate (stock solution: 100 mg in
500 mL H2O) per liter of media. The antibiotics
should be filter sterilized prior to use. To enhance
starch hydrolysis zones, add 30 µl L -1 of methyl
violet 2B (1% solution in 20% ethanol), and 60 µl
L -1 of methyl green (1% aqueous solution).
The medium was utilized to characterize
X. campestris pv pennamericanum, the causal
agent of bacterial leaf streak of pearl millet
(Qhobela and Claflin 1987).
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