ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ... ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
A.2.1 Introduction A.2.2 A.2.2.1 A.2.2.2 CHAPTERA-2 MATERIALS AND METHODS This chapter gives a briefdescription ofthe materials and methods used to determine the nutritional and anti-nutritional composition of Colocasia esculenta grown in Zululand, South Africa. See Appendices A and B for details. Materials Food material Two varieties of Colocasia esculenta L. Schott (Amadumbe) - white and purple - were obtained from local markets at Esikhawini and Mtubatuba and from Makatini Experimental Farm, KwazuIu-Natal (KZN), South Africa Reagents (See Appendix A for details ofhow reagents were prepared.) a-Amylase a-Chymotrypsin from human pancreas l3-glycosidase Ammonium molybdate Anthrone reagent Ascorbic acid Benzoyl-DL-arginine-p-nitroaniline (BAPNA) Bispyrazolone 33 Sigma Sigma Sigma Kleber chemicals Merck Merck Sigma Fluka
- Page 1 and 2: ANTI-NUTRITIONAL CONSTITUENT OF COL
- Page 3: ABSTRACT Colocasia esculenta 1. Sch
- Page 6 and 7: • My colleagues Kayode, Stranger,
- Page 8 and 9: CHAPTER A-2 : MATERIALS AND METHODS
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- Page 12 and 13: C.3.3 EXPERIMENTAL TEST C.3.3.1 Foo
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- Page 57 and 58: A.2.3.5 A.2.3.6 A.2.3.6.1 A.2.3.6.2
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- Page 100 and 101: REFERENCES Adeparusi E.O. (2001) Ef
A.2.1 Introduction<br />
A.2.2<br />
A.2.2.1<br />
A.2.2.2<br />
CHAPTERA-2<br />
MATERIALS AND METHODS<br />
This chapter gives a briefdescription ofthe materials and methods used to determine the<br />
nutritional and anti-nutritional composition of Colocasia esculenta grown in Zululand,<br />
South Africa. See Appendices A and B for details.<br />
Materials<br />
Food material<br />
Two varieties of Colocasia esculenta L. Schott (Amadumbe) - white and purple - were<br />
obtained from local markets at Esikhawini and Mtubatuba and from Makatini<br />
Experimental Farm, KwazuIu-Natal (KZN), South Africa<br />
Reagents<br />
(See Appendix A for details ofhow reagents were prepared.)<br />
a-Amylase<br />
a-Chymotrypsin from human pancreas<br />
l3-glycosidase<br />
Ammonium molybdate<br />
Anthrone reagent<br />
Ascorbic acid<br />
Benzoyl-DL-arginine-p-nitroaniline (BAPNA)<br />
Bispyrazolone<br />
33<br />
Sigma<br />
Sigma<br />
Sigma<br />
Kleber chemicals<br />
Merck<br />
Merck<br />
Sigma<br />
Fluka
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