ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ... ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
180 = molecular weight ofthe glucose; 60 = incubation time in minutes; N = number ofmolecules ofglucose hberated by hydrolysis in each sugar (n = 1 for sucrose and lactose, n = 2 for maltose) [Rodriguez-Castilla et al., 1996]. B.C.2.3 ATPase [Vaslirhelyi et aL (1986)] The Na+!K'"-ATPase activity of freeze-thawed samples was determined by measuring the release of inorganic phosphate (Pi) associated with the hydrolysis of ATP. The samples (250 )l1) were added to 4750 )l1 of Reagent 1 [final concentration per litre: 100 mM of NaCl., 20 mM of KCl., 2.5 mM of MgCh, 0.5 mM of ethylene glycol tetraacetic acid (EGTA), 50 mM oftris-HCI (pH 7.4),1 mM ofATP, 1 mM of phosphoenolpyruvate, 0.16 mM of nicotineamide adenine dinucleotide (NADH), 5 kU of pyruvate kinase, 12 kU of lactate dehydrogenase]. After 300 s, 65 J.L1 of 10 mM ouabain was added to inhIbit ouabain sensitive ATPase activity. The Na+JK+-ATPase is composed of the stoichiometric of two obligatory major polypeptides, the a-subunit (- 112 kDa) and the f3-subunit (- 45 kDa). The binding sites for ATP, cations and ouabain are localized in the a-subunit, which is responsible for the atalytic activity ofthe enzyme. The change in absorbance was monitored at 340 llID. The Na+JK+-ATPase activity was calculated from the difference in A 340 . 257
AppendixC OLEIC ACID STANDARD 230 235 240 258 l:TOFMS;j 'ol
- Page 223 and 224: .. - Figure C3-5: Representative ph
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- Page 229 and 230: C.4.1 Introduction CHAPTERC-4 DISCU
- Page 231: significantly, decreased after beta
- Page 234 and 235: decreased by sitosterols as a resul
- Page 237 and 238: Bhattacharyya AI
- Page 239 and 240: Dablqvist A (1968) Assay ofintestin
- Page 242 and 243: Ivorra MD., D'OconMP., Paya M and V
- Page 245 and 246: Miettinen TA, Tilvis RS. and Kesani
- Page 247: Pollak O.I. and Kritchevsky D. (198
- Page 251 and 252: 2.1 Introduction CHAPTER 2 GENERAL
- Page 253 and 254: 2.4 Suggestions for future work Fur
- Page 255: A.A.3 Enzyme trypsin 10 mg bovine-p
- Page 259 and 260: AppendixB DETAILS OF MEmOOOLOGY BA1
- Page 261 and 262: sugars were estimated from standard
- Page 263 and 264: B.A.l.5.4.5 Liquid Chromatography M
- Page 266: B.A.2.4 Tannin [Van-Burden and Robi
- Page 269 and 270: The mixture was stirred continuousl
- Page 273: B.C.l Esterification ofbeta-sitoste
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AppendixC<br />
OLEIC ACID STANDARD<br />
230 235 240<br />
258<br />
l:T<strong>OF</strong>MS;j<br />
'ol
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