ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...

A.A.ll 0.1 M Orthophosphorieletbanol medium
6.78 mI of orthophosphoric acid was dissolved in I litre of distilled water and 250 mI of
ethanol was added to the orthophosphoric acid.
AB.l Phosphate buffer
18.74 g of sodium dihydrogen orthophosphate ( ) and 8.16 g of disodium hydrogen
orthophosphate ( ) were mixed and dissolved together in a litre ofdistilled water. pH was
adjusted to 6.9.
AB.2 Soluble starch
I g ofsoluble starch was dissolved in 100 mI ofphosphate buffer.
AB.3 Dinitrosalicylic acid
1 g of3.5 dinitrosalicylic acid was dissolved in 20 mI of2 M NaOH and 50 mI of distilled
water. 30 g of Rochelle salt was added and the resulting mixture was made up to 100 ml
with distilled water.
AB.4 Tris-HO
0.72 mI HCl was added to 10 mI of distilled water. 6.06 g ofTris was dissolved in 200 mI
of distilled water and 9.75 ml of HCl. The resulting mixture was made up to 250 m1, then
up to 1000 mI with distilled water and the pH was adjusted to 7.5.
AB.5 0.4 M NaO phosphate buffer
23.38 g ofNael was dissolved in 500 mI ofphosphate buffer.
AB.6 G-l00 Sephadex
109 ofSephadex was dissolved in 500 mI ofphosphate buffer.
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