ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...

C.2.1 Introduction
CHAPTERC-2
MATERIALS AND METHODS
Two types of experiments were conducted for the nutritional evaluation. A six-day,
short-term, preliminary toxicity test was Used to determine the dietary concentration of
beta-sitosterol for the two-week experimental test This chapter explains the
experimental design, with materials and methods for each ofthe parameters tested.
C.2.2 Materials and methods
C2.2.1
'::.2.2.2
Beta-sitosterol
Oleic acid
Sodium caseinate
Glucose (GO) Assay Kit
Ethical consideratiou
Sigma
Sigma
Sigma
Sigma
The experimental protocol was performed according to national and international
guidelines. The animal experiment was approved by the Ethics Committee of the
Faculty of Science and Agriculture of the University of Zululand, KZN, South Africa.
(See Appendix C).
Solubilisation of beta-sitosterol
Beta-sitosterol was solubilised by esterification in the presence of sodium caseinate
solution (US Patent 6491952). Beta-sitosterol was administered as a suspension in oleic
acid (Sjoberg, 2002).
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