ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ... ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
CHAPTER 1: GENERAL INTRODUCTION Nutritional value is the main concern when a plant is considered as food source. However, endogenous toxic :fuctors characteristic of plant material can also affect the content of nutrients. These toxic factors act as anti-nutrients and adversely affect the organism. Anti nutrients are chemicals which have been evolved by plants for their own defence, among other biological functions. Anti-nutrients reduce the maximum utilization of nutrients (especially proteins, vitamins and minerals), thus preventing optimal exploitation of the nutrients present in a food and decreasing the nutritive value (Ugwu and Oranye, 2006). Anti-nutrients vary in chemical structures, ranging from amino acids to proteins; from simple amines to a1kaloids, glycosides and many phenolic compounds. The biological effects ofall these chemicals are diverse and complex. When man ingests plant foods to meet nutritional needs, a wide variety of these non nutrient phytochemicals are ingested at the same time. Processing is expected to inactivate these anti-nutritional factors and increase the availability of bioactive compounds. However, the health risk to consumers of large quantities of residual anti-nutrients cannot be ruled out In sub-Saharan Africa, approximately three quarters of the population live in rural areas. Most of their dietary energy comes from staple cereals, such as maize. Given the limited resources and restricted access to different foods in rural societies, most African communities have developed diets that maximize the use of local foodstuffs. The advancements in grain production have not brought significant benefits to areas where tuber crops are the major staples. Therefore, emphasis should be placed on such tuber crops as Amadumbe (Colocasia esculenta), which is a staple food in many developing nations of West-Africa (as taro/cocoyam), Asia and the Pacific (as cocoyam). Amadumbe (Colocasia esculenta) is widely grown in the sub-tropical parts of South Africa as a traditional food I
crop. Amadumbe is not extensively commercialized at present, but is mostly grown in rural areas or on small fimns in Kwazulu-Natal, South Africa. Literature abounds with research carried out on Colocasia esculenta species in other parts ofthe world, but little or no information is available regarding the composition, structural activity and biochemical mechanisms of anti-nutritional factors in local Colocasia esculenta (Amadumbe). Studies investigating these factors could help the government in formulating food and nutrition policies for South Africa. The overall hypothesis of this study was, therefore, that if the constituents of various nutritional and anti-nutritional factors inherent in Colocasia esculenta (Amadumbe), a traditional crop food grown in Kwazulu-Natal, were identified, this tuber could be more efficiently utilized in food and nutrition policies for South Africa. The project was divided into three specific aims to meet the overall objective: • to determine the proximate composition, the mineral and the anti-nutrient content of Amadumbe from Kwazulu-Natal, South Africa, in order to compare the factors with those observed in Colocasia esculenta from other areas ofthe world. At the same time, the best processing method for maximum elimination of the anti-nutritional factors would be identified; • to isolate and characterize some ofthe screened anti-nutritional factors to determine the structural activity and biochemical properties of these toxicants occurring naturally in Amadumbe tubers; • to perform a nutritional evaluation using rats with a specific, identified anti-nutritional factor from Colocasia esculenta to determine the biological effects of the anti nutritional factoL 2
- Page 1 and 2: ANTI-NUTRITIONAL CONSTITUENT OF COL
- Page 3: ABSTRACT Colocasia esculenta 1. Sch
- Page 6 and 7: • My colleagues Kayode, Stranger,
- Page 8 and 9: CHAPTER A-2 : MATERIALS AND METHODS
- Page 10 and 11: CHAPTER 8-1-4 : DISCUSSION Bl-A.l I
- Page 12 and 13: C.3.3 EXPERIMENTAL TEST C.3.3.1 Foo
- Page 14: MakP Makatini purple MakW Makatini
- Page 17 and 18: Figures C3-9 Figure B-1 The activit
- Page 19: Table C3-3 TableC3-4 Summary ofseru
- Page 24 and 25: section of the study therefore inve
- Page 26 and 27: Figure AI-2: The aboveground portio
- Page 28 and 29: Taro is produced in the Pacific Isl
- Page 30 and 31: A.1.4 Anti-nutrients Foods are comp
- Page 32 and 33: essential amino acids not available
- Page 34 and 35: A.l.4.2 Amylase inhibitors In order
- Page 36: A.l.4.4 Total polyphenols Polypheno
- Page 42: A.1.4.7 when the levels are high en
- Page 45: A.1.4.8 coordination sides to inhib
- Page 49 and 50: However,ifsaponins have a triterpen
- Page 51 and 52: A.l.6 nutritional factors naturally
- Page 54 and 55: A.2.2.3 A.2.3 A.2.3.1 A.2.3.2 Speci
- Page 56 and 57: A.2.3.4.2 A.2.3.4.3 A2.3.4.4 In ano
- Page 58: A.2.3.6.3 \.2.3.6.3.1 \.2.3.6.3.2 L
- Page 61 and 62: sulphuric acid. Absorbance was meas
- Page 63 and 64: Table A3-1a: The moisture and ash c
- Page 65 and 66: Table A3-1c Carbohydrate content (g
- Page 68 and 69: and 4.13 mg 100 g-l DMin the unproc
CHAPTER 1: GENERAL INTRODUCTION<br />
Nutritional value is the main concern when a plant is considered as food source. However,<br />
endogenous toxic :fuctors characteristic of plant material can also affect the content of<br />
nutrients. These toxic factors act as anti-nutrients and adversely affect the organism. Anti<br />
nutrients are chemicals which have been evolved by plants for their own defence, among<br />
other biological functions. Anti-nutrients reduce the maximum utilization of nutrients<br />
(especially proteins, vitamins and minerals), thus preventing optimal exploitation of the<br />
nutrients present in a food and decreasing the nutritive value (Ugwu and Oranye, 2006).<br />
Anti-nutrients vary in chemical structures, ranging from amino acids to proteins; from<br />
simple amines to a1kaloids, glycosides and many phenolic compounds. The biological<br />
effects ofall these chemicals are diverse and complex.<br />
When man ingests plant foods to meet nutritional needs, a wide variety of these non<br />
nutrient phytochemicals are ingested at the same time. Processing is expected to inactivate<br />
these anti-nutritional factors and increase the availability of bioactive compounds.<br />
However, the health risk to consumers of large quantities of residual anti-nutrients cannot<br />
be ruled out<br />
In sub-Saharan Africa, approximately three quarters of the population live in rural areas.<br />
Most of their dietary energy comes from staple cereals, such as maize. Given the limited<br />
resources and restricted access to different foods in rural societies, most African<br />
communities have developed diets that maximize the use of local foodstuffs. The<br />
advancements in grain production have not brought significant benefits to areas where<br />
tuber crops are the major staples. Therefore, emphasis should be placed on such tuber crops<br />
as Amadumbe (Colocasia esculenta), which is a staple food in many developing nations of<br />
West-Africa (as taro/cocoyam), Asia and the Pacific (as cocoyam). Amadumbe (Colocasia<br />
esculenta) is widely grown in the sub-tropical parts of South Africa as a traditional food<br />
I