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ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...

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B2.2.1<br />

B2.2.2<br />

B2.2.3<br />

Introduction<br />

CHAPTER B2-2<br />

MATERIALS AND METHODS<br />

This chapter gives a brief description of the materials and methods used to isolate,<br />

partially purify and characterize a saponin from white Colocasia esculenta grown in<br />

Esikhawini, Zululand, South Africa. (See Appendix B for methodology).<br />

Plant material<br />

White tubers from Colocasia esculenta (Amadmnbe) were collected from the local<br />

market at Esikhawini, Kwazulu-Natal, South Africa. The plant materials were collected<br />

between January and March 2006.<br />

Methods<br />

(See Appendix B for details ofmethodology)<br />

147

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