ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...

BI.3.1
BI.3.2.1
Introduction
CHAPTER BI-3
RESULTS
In this chapter, the results for the isolation, partial purification and characterization ofthe
two a-amylase inhibitors from Amadumbe are presented.
Extraction and purification ofa-Amylase inhibitors
The overall results of the purification procedure of a-amylase inlnbitors from Colocasia
esculenta have been summarized in Table BI-3.1. Preliminary studies (Figure B-1)
suggested that 80 per cent ammonium-sulphate saturation was best for salting out the
inhibitor protein from the crude extract.
Table BI-3.1: Purification ofamylase inhibitors from Amadumbe
Crude extract
Ammonium sulphate
DEAE sephacel:
Peak A
PeakB
Sephadex G-lOO
Peak Al
PeakBl
20.9
18.5
7.2
5.9
3.7
5.6
62
56
54
50.5
41.8
39.8
2.9
3.02
7.5
8.56
11.29
12.83
Note: The inhibitor units were calculated using human-salivary amylase.
129
100
88.5 1.04
33.44
28.22
17.70
14.83
2.59
2.93
3.89
4.42