ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ... ANTI-NUTRITIONAL CONSTITUENT OF COLOCASIA ESCULENTA ...
diabetes mellitus or obesity. Octivio and Rigden (2000) referred to a-amylase and its inhibitors as 'drug-design targets' from which compounds for the treatment of diabetes, obesity and hyperlipaemia could be developed. Btl.3 Aim and outline ofSection B-1 Amylase inlnbitors have been extracted from several types of plants, especially those in the cereal and legume family. In contrast to a-amylase inhibitors in cereal (Roy and Gupta, 2000; Heidari et al., 2005; Muralikrishna and Nirma1a, 2005) and legumes (Giri and Kachole, 1998; Melo et ai, 1999), which have been extensively studied, little is known about the structural features and properties oftuber a-amylase inhibitors. The aim of Section B-1 was therefore to extract, isolate and characterize a-amylase inhibitors from Amadumbe. The objectives were: • the extraction, isolation and partial purification ofa-amylase inhibitors through ion-exchange and gel chromatography; • to undertake kinetic studies (pH and temperature optima); and • to investigate the selectivity ofinhibitor action on different a-amylases. 124
B1.2.1 Introduction B1.2.2 Materials B1.23 Methods CHAPTER BI-2 MATERIALS AND METHODS This chapter gives a brief description of the materials and methods used to isolate, partially purify and characterize a-amylase inhibitors from white Colocasia esculenta grown in Esikhawini, Zululand, South Africa. (See Appendices A and B for details). The white variety of Amadumbe (Colocasia esculenta) tubers was obtained from the local maIket in Esikhawini, Kwazulu-Nata1, South-Africa. Commercially available amylases (human saliva, type IX-A; porcine pancreatic, type I-A; sweet potato, barley, Bacillus species, type IT-A; Aspergillus oryzae) and all other reagents used were obtained from Sigma Chemical Company. (See Appendix B for details ofmethodology.) 125
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B1.2.1 Introduction<br />
B1.2.2 Materials<br />
B1.23 Methods<br />
CHAPTER BI-2<br />
MATERIALS AND METHODS<br />
This chapter gives a brief description of the materials and methods used to isolate,<br />
partially purify and characterize a-amylase inhibitors from white Colocasia esculenta<br />
grown in Esikhawini, Zululand, South Africa. (See Appendices A and B for details).<br />
The white variety of Amadumbe (Colocasia esculenta) tubers was obtained from the<br />
local maIket in Esikhawini, Kwazulu-Nata1, South-Africa. Commercially available<br />
amylases (human saliva, type IX-A; porcine pancreatic, type I-A; sweet potato, barley,<br />
Bacillus species, type IT-A; Aspergillus oryzae) and all other reagents used were obtained<br />
from Sigma Chemical Company.<br />
(See Appendix B for details ofmethodology.)<br />
125
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