Do it quick! Update on CLSI M35A2 - SWACM

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Technologist must…. Begin w<strong>it</strong>h pure colony Recognize what <strong>it</strong> could be from typical colony morphology Perform rapid tests accurately and read them correctly <strong>Do</strong>n’t cut corners…
Supervisor must….. Validate the competency of the staff doing tests Check to see that all tests are done Be sure that procedures are wr<strong>it</strong>ten and QC is done usually by lot
Page 1 and 2: Do it</str
Page 3 and 4: References Prove Value : D
Page 5 and 6: Grows on MAC Spreading Proteus
Page 7 and 8: M35-A2 Nov. 2009 M35A GRAM-POSITIVE
Page 9: “Is Use of Odor Safe” “Sniff
Page 13 and 14: Not “Presumptive” >95% accurac
Page 15 and 16: Many rapid indole tests….
Page 17 and 18: PYR negative = E. coli 1. Wet disk
Page 19 and 20: Brucella blood, lymph nodes, body f
Page 21 and 22: Urease rapid test-incubate 2 h - +
Page 23 and 24: Other Oxidase + catalase + urease +
Page 25 and 26: Campylobacter in stools Distinctiv
Page 27 and 28: Positive Indoxyl A
Page 29 and 30: Colonies move when pushed
Page 31 and 32: HACEK bacteria BAP & MAC Pos<strong
Page 33 and 34: Cardiobacterium hominis Indole-pos
Page 35 and 36: Blood agar hemolysis- Found in join
Page 37 and 38: Francisella tularensis If growth ta
Page 39 and 40: Rapid identification Oxidase - Cata
Page 41 and 42: Francisella tularensis - BSL III
Page 43 and 44: Neisseria species Gram-negative di
Page 45 and 46: Neisseria lactamica Colony grows on
Page 48 and 49: Next step if not eit</stron
Page 50 and 51: Neisseria Table All are catalase +
Page 52 and 53: Staphylococcus Latex tests Staph L
Page 54 and 55: S. lugdunensis can be Latex + or -
Page 56 and 57: Algorithm for Stap
Page 58 and 59: PYR-positive α he
Page 60 and 61:
•Streptococcus anginosus (“mill
Page 62 and 63:
Listeria monocytogenes (blood, CSF,
Page 64:
Rapid testing does make a differenc
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Do it quick! Update on CLSI M35A2 - SWACM

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