Susana Isabel Ferreira da Silva de Sá ESTROGÉNIOS E ...

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310 Fig. 1. Electron micrographs of three consecutive ultrathin sections (A–C) of the VMNvl showing identical areas of the neuropil. A dendritic shaft (d) bears a large spine (s) that makes an asymmetrical synaptic contact (transparent arrow) with an axon terminal (a). This synaptic contact can be seen only in A and B. Axon terminals contacting dendritic shafts at asymmetrical synapses (thick arrows) can be seen in A. Asymmetrical spine synapses that are visible in one micrograph but not in the adjacent micrographs are marked by thin arrows. Scale bar0.74 m. Uterine weight To confirm the efficacy of the estradiol injection, we measured the uterine weight of rats used in both experiments (Table 1). We found a significant overall effect on uterine 44 S. I. Sá et al. / Neuroscience 162 (2009) 307–316 weight across treatment groups (F 7,4019.74; P0.0005). Animals injected with EB, i.e. belonging to EB, 2 EB, EBP and EBRU groups had higher uterine weights than rats treated with either oil or progesterone alone. In females injected with the ER agonist PPT the uterine
Table 1. Serum estradiol and progesterone levels, uterine weight and lordosis intensity in rats belonging to the different groups analyzed in Experiments 1 and 2 weight was similar to that of estradiol-treated rats and, accordingly, was significantly higher than in oil-treated rats. Rats treated with DPN had uterine weights similar to those of rats treated with oil or progesterone alone. Progesterone administration had no statistically significant effect (P0.05) on uterine weight compared to oil-treated rats. Numerical density of synapses Estradiol (pg/ml) Progesterone (ng/ml) Uterine weight (g) Lordosis intensity O group 33.17 (3.14) 4.02 (0.22) 0.25 (0.03) 0 P group 33.70 (0.71) 14.52 (0.84) 0.24 (0.02) 0 EBP group 59.55 (4.49)* 13.48 (0.59) 0.91 (0.09) ## 2.5 (0.22) EBRU group 64.76 (9.14)** 4.69 (0.14) 1.10 (0.16) ## 0 EB group 63.47 (5.87)* 5.82 (0.66) 0.89 (0.07) ## 0 2 EB group 110.61 (7.09) xxx 5.56 (0.64) 0.79 (0.05) # 0 PPT group 34.17 (4.09) 3.63 (0.54) 0.81 (0.08) ## 0 DPN group 33.23 (3.90) 4.40 (0.60) 0.25 (0.21) 0 Values are expressed as means (SEM). * P0.05; ** P0.005, compared with groups that were not injected with estradiol; xxx P0.0005 compared with all other groups; P0.0005 compared with groups that were not injected with progesterone; # P0.005, ## P0.0005 compared with O-, P- and DPN-treated groups. In Experiment 1 (Fig. 2), in which O, P, EB, EBP and EBRU groups were studied, we found no significant influence of treatment in the N V of spine (F 4,252.85; P0.05) and dendritic (F 4,251.75; P0.17) synapses. In contrast, in Experiment 2 (Fig. 3), in which O, EB, 2 EB, PPT and DPN groups were analyzed, ANOVA revealed a significant effect of treatment on the N V of both types of synapses (F 4,2519.90; P0.0001 for spine and F 4,25 7.63; P0.0004 for dendritic synapses). With respect to spine synapses, the density was significantly higher in 2 EB- and PPT-treated rats than in the remaining groups. In dendritic synapses, this difference was only apparent for the PTT-treated group and no significant difference was found between 2 EB-treated rats and the remaining groups. Fig. 2. Graphic representation of the N V of spine (white columns) and dendritic (black columns) synapses in the VMNvl of rats studied in Experiment 1. The number of animals per group was six. On average, 200 synapses were counted per animal. Columns represent means and vertical bars 1 SD. S. I. Sá et al. / Neuroscience 162 (2009) 307–316 311 Neuronal numerical density ANOVA revealed a significant influence of treatment in the N V of VMNvl neurons in Experiment 1 (F 4,255.70; P 0.005) as well as in Experiment 2 (F 4,257.46; P0.0005). In Experiment 1 (Fig. 4), rats injected with estradiol (EB, EBP and EBRU groups) had significantly fewer neurons per unit volume of the VMNvl than rats injected with oil or progesterone (O and P groups). In Experiment 2 (Fig. 4), rats treated with estradiol (EB and 2 EB groups) and rats receiving ER agonists (PPT and DPN groups) had significantly lower neuronal numerical densities than oiltreated rats. No significant differences were detected among EB, 2 EB, PPT and DPN groups. Total number of synapses per neuron The variations in synapse numbers induced by the administration of hormones, and receptor agonists and antagonists are shown in Figs. 5 and 6 and summarized in the diagram shown in Fig. 7. Treatments used in Experiment 1 significantly influenced the total number of spine (F 4,259.57; P0.0001) and dendritic (F 4,257.73; P0.001) synapses established by each VMNvl neuron (Fig. 5). The number of Fig. 3. Graphic representation of the N V of spine (white columns) and dendritic (black columns) synapses in the VMNvl of rats studied in Experiment 2. The number of animals per group was six. On average, 200 synapses were counted per animal. Columns represent means and vertical bars 1 SD. Tukey’s post hoc tests: * P0.05, ** P0.0005 compared with O-treated group; P0.005, P0.0005 compared with EB-treated group; # P0.005 compared with DPN-treated group. 45
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Susana Isabel Ferreira da Silva de
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Esta investigação foi realizada n
Page 9 and 10:
Corpo Catedrático da Faculdade de
Page 11: Doutor Manuel Machado Rodrigues Gom
Page 15 and 16: AGRADECIMENTOS Em 2001, por sugest
Page 17: ÍNDICE ITRODUÇÃO 1 TRABALHOS 15
Page 21 and 22: Acção dos estrogénios no sistema
Page 23 and 24: O núcleo ventromedial do hipotála
Page 25 and 26: ventral e sistema mesencefálico de
Page 27 and 28: 1991). São, no entanto, menos clar
Page 29: o objectivo de aquilatar se as dose
Page 33: TRABALHOS 15
Page 37 and 38: Neuroscience 133 (2005) 919-924 NEU
Page 39 and 40: enclosed by the Golgi complex (Fig.
Page 41 and 42: Fig. 4. Graphic representation of t
Page 43: Estrogen modulates the sexually dim
Page 46 and 47: SYNAPTIC PLASTICITY IN THE VM NUCLE
Page 59 and 60: Neuroscience 162 (2009) 307-316 EFF
Page 61: ml) and chloral hydrate (40 mg/ml)
Page 65 and 66: (Frankfurt and McEwen, 1991a; Segar
Page 67 and 68: Canteras NS, Simerly RB, Swanson LW
Page 69: Role of neural afferents as mediato
Page 72 and 73: 2 1989; Krettek and Price, 1978), b
Page 74 and 75: 4 rats. As expected, treatment of o
Page 76 and 77: 6 estrogen-induced synapse formatio
Page 78 and 79: 8 4.3. Hormonal determinations Bloo
Page 80 and 81: 10 estrogen treatment. Brain Res. 2
Page 83: DISCUSSÃO GERAL 65
Page 86 and 87: Apesar de nas fêmeas os neurónios
Page 88 and 89: influências excitatórias que sobr
Page 90 and 91: estrogénios promovam a formação
Page 93: CONCLUSÕES 75
Page 97: REFERÊNCIAS 79
Page 100 and 101: Brown TJ, Clark AS, MacLusky NJ. Re
Page 102 and 103: Griffin GD, Flanagan-Cato LM. Estra
Page 104 and 105: Luiten PG, ter Horst GJ, Steffens A
Page 106 and 107: Ribeiro AC, Sawa E, Carren-LeSauter
Page 108 and 109: Voskuhl RR. Hormone-based therapies
Page 111: Com os resultados constantes desta
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The studies incorporated in the pre
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