Incidence, Distribution and Characteristics of Major Tomato Leaf ...

Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases
For TAS-ELISA (Macintosh et al., 1992; Roberts et al., 1984), a monoclonal antibody
developed by Dr. Steven Walker (Germany) against TYLCV-SR and named TYLCV -
4F 11 C5-C3, together with ACMV IgG, was used to test samples. Nunc® microtiter
plates were used.
Table 3.4: Cultivated crops and weeds bearing leaf curl and mosaic symptoms in and
around tomato fields of Kabale and Kasese districts, and tested for TYLCV in the
laboratory
Plant Source Description
Solanum melongena Kabale Highland Leaf mottling symptoms
Cucurbita maxima Kasese Irrigation
Scheme
Severe mosaic symptoms
Nicotiana tabacum Kasese Irrigation
Scheme
Leaf curl, mottling and stunting
Desmodium spp. Kasese Irrigation Small leaves with interveinal
Scheme
yellowing
Commelina benghalensis Kasese Irrigation
Scheme
Small leaves with mottling
Conyza floribunda Kabale Highland Leaf curl and crinkling
Crassocephalum spp. Kabale Highland Crinkled leaves with mottling
Euphorbia heterophylla Kasese Irrigation Small curled leaves with
scheme
whiteflies
Ageratum conyzoides Kabale Highland Mild mottling
Table 3.5: Weeds tested for tomato leaf curl virus (ToLCV-UG) begomovirus: these
weeds had yellow leaf curl symptoms, and were therefore collected from Iganga and
Mbarara districts
Weed Source Description
Euphorbia heterophylla Iganga Small leaves with whiteflies
Physalis floridana Iganga Small leaves, curl, yellow mottle
Desmodium spp. Mbarara Small leaves with yellowing
Samples were squashed in eppendorf tubes using a surface-sterilised prototype of Kontes
pestles developed from plastic comb teeth with a broad base and small tip. Each sample
was replicated twice on the microtiter plate and each test repeated twice. A sample was
considered positive if both replicates turned obviously yellow in microtiter plate wells,
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