Incidence, Distribution and Characteristics of Major Tomato Leaf ...

Incidence, Distribution and Characteristics of Major Tomato Leaf ... Incidence, Distribution and Characteristics of Major Tomato Leaf ...

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Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases Eretmocerus spp., as well as predators like lacewing and coccinelids (Zalon et al., 2003). However, concentrating on vector control is not always very effective since some vectors transmit viruses in a persistent manner (Green, 1991; Gianessi et al., 2003), so that even at very low densities, some vectors are effective in transmitting viruses. Moreover, transmission of non-persistent viruses takes place quickly and readily, and is often performed by vectors that visit, but do not establish their colonies on the visited crop. According to Perring et al. (1999), no one method of control is likely to keep crops free of vectors and virus infections. 2.1.4.1.1 Crop Sanitation A number of cultural practices have been reported to reduce incidence and severity of viral diseases. These include elimination of inoculum sources by removal of infected plants and alternative hosts. Chan and Jeger (1994), while working with an analytical model of plant virus disease dynamics with uprooting and replanting, found that in sparsely planted crops intensive uprooting only was able to eradicate virus diseases. Cho et al. (1989) reported that removing TSWV-infected crop plants, weeds and alternative hosts, in an overall integrated management package, helped to control viral diseases. Lloyd et al. (1974) were able to eliminate banana viruses by use of virus-free planting material obtained by thermal therapy and tissue culture. Other effective methods reported are debris removal from the field, disinfection of support trellises before re-use, crop quarantine measures executed for incoming seeds, and crop rotation (AVRDC, 1985; Green and Kim, 1991); use of skimmed milk foliar sprays (Black et al., 1991; Green et al., 1991); application of antiviral agents like cytovirin (Simmons 1959), and treatment of tomato seed with trisodium phosphate for 1 hour (Broadbent, 1965 and 1976). 2.1.4.1.2 Cultural Control There are a number of cultural control measures, such as close plant spacing to compensate for yield losses through diseased plants, use of mulching and intercropping with nitrogen fixing cover crops like Lablab purpureus L. (Cohen et al., 1974). Greer and 42

Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases Dole (2003) reported that aluminium foil and mulches are effective in repelling insect pests, and that black plastic mulches improve crop yield better than bare ground. Mutwiwa et al. (2005) found that ultra-violet light absorbing plastics used as mulches or green house roofing material repel whitefly pests when low UV-intensity plastics are used. Other cultural options include timely planting, intercropping, and use of barrier hedges (Simons et al., 1980). These methods effectively reduce tomato yield losses, virus incidence and spread, even though they are simple and common. 2.1.4.2 Cross-protection Cross-protection is the method of controlling virus disease by using a mild virus strain to immunize otherwise healthy plants against a severe virus strain (Matthews, 1991). The method has been used against ToMV and TSWV. Avgelis (1987) used a mild strain (MX lV – l) of ToMV to cross-protect ToMV-susceptible tomato cultivars Earlypak and Dombo C2 VF2 in Crete, leading to 14% yield improvement. He expected higher yields, and as such attributed low increase in yield to higher than expected temperatures, which could have reduced the effectiveness of mild strains. Another example of cross-protection is when Min-Wang and Gonsalves (1992) and Cho et al. (1989) used nitrous acid to induce mutation of TSWV to mutant R27G, which was put in tomato in order to control severe TSWV strains from Hawai and Oklahoma. This method requires more sophisticated facilities and careful management of the outcome, which could otherwise result into more dangerous strains. With limited resources in the south, one would to be very careful to recommend use of such a technology, even though it could be required in some instances. 2.1.4.3 Host Plant Resistance Use of resistant crop varieties is the most convenient and cost-effective control measure of all (Hall, 1980; Gajos, 1981; Kumar and Irulappan, 1992; Nono-Womdim, 1993; Rubio et al., 2003; Yang et al., 2004; de Castro et al., 2005). For example, planting of 43

<strong>Incidence</strong>, distribution <strong>and</strong> characteristics <strong>of</strong> major tomato leaf curl <strong>and</strong> mosaic virus diseases<br />

Eretmocerus spp., as well as predators like lacewing <strong>and</strong> coccinelids (Zalon et al., 2003).<br />

However, concentrating on vector control is not always very effective since some vectors<br />

transmit viruses in a persistent manner (Green, 1991; Gianessi et al., 2003), so that even<br />

at very low densities, some vectors are effective in transmitting viruses. Moreover,<br />

transmission <strong>of</strong> non-persistent viruses takes place quickly <strong>and</strong> readily, <strong>and</strong> is <strong>of</strong>ten<br />

performed by vectors that visit, but do not establish their colonies on the visited crop.<br />

According to Perring et al. (1999), no one method <strong>of</strong> control is likely to keep crops free<br />

<strong>of</strong> vectors <strong>and</strong> virus infections.<br />

2.1.4.1.1 Crop Sanitation<br />

A number <strong>of</strong> cultural practices have been reported to reduce incidence <strong>and</strong> severity <strong>of</strong><br />

viral diseases. These include elimination <strong>of</strong> inoculum sources by removal <strong>of</strong> infected<br />

plants <strong>and</strong> alternative hosts. Chan <strong>and</strong> Jeger (1994), while working with an analytical<br />

model <strong>of</strong> plant virus disease dynamics with uprooting <strong>and</strong> replanting, found that in<br />

sparsely planted crops intensive uprooting only was able to eradicate virus diseases. Cho<br />

et al. (1989) reported that removing TSWV-infected crop plants, weeds <strong>and</strong> alternative<br />

hosts, in an overall integrated management package, helped to control viral diseases.<br />

Lloyd et al. (1974) were able to eliminate banana viruses by use <strong>of</strong> virus-free planting<br />

material obtained by thermal therapy <strong>and</strong> tissue culture. Other effective methods reported<br />

are debris removal from the field, disinfection <strong>of</strong> support trellises before re-use, crop<br />

quarantine measures executed for incoming seeds, <strong>and</strong> crop rotation (AVRDC, 1985;<br />

Green <strong>and</strong> Kim, 1991); use <strong>of</strong> skimmed milk foliar sprays (Black et al., 1991; Green et<br />

al., 1991); application <strong>of</strong> antiviral agents like cytovirin (Simmons 1959), <strong>and</strong> treatment <strong>of</strong><br />

tomato seed with trisodium phosphate for 1 hour (Broadbent, 1965 <strong>and</strong> 1976).<br />

2.1.4.1.2 Cultural Control<br />

There are a number <strong>of</strong> cultural control measures, such as close plant spacing to<br />

compensate for yield losses through diseased plants, use <strong>of</strong> mulching <strong>and</strong> intercropping<br />

with nitrogen fixing cover crops like Lablab purpureus L. (Cohen et al., 1974). Greer <strong>and</strong><br />

42

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