Incidence, Distribution and Characteristics of Major Tomato Leaf ...
Incidence, Distribution and Characteristics of Major Tomato Leaf ... Incidence, Distribution and Characteristics of Major Tomato Leaf ...
Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases Eretmocerus spp., as well as predators like lacewing and coccinelids (Zalon et al., 2003). However, concentrating on vector control is not always very effective since some vectors transmit viruses in a persistent manner (Green, 1991; Gianessi et al., 2003), so that even at very low densities, some vectors are effective in transmitting viruses. Moreover, transmission of non-persistent viruses takes place quickly and readily, and is often performed by vectors that visit, but do not establish their colonies on the visited crop. According to Perring et al. (1999), no one method of control is likely to keep crops free of vectors and virus infections. 2.1.4.1.1 Crop Sanitation A number of cultural practices have been reported to reduce incidence and severity of viral diseases. These include elimination of inoculum sources by removal of infected plants and alternative hosts. Chan and Jeger (1994), while working with an analytical model of plant virus disease dynamics with uprooting and replanting, found that in sparsely planted crops intensive uprooting only was able to eradicate virus diseases. Cho et al. (1989) reported that removing TSWV-infected crop plants, weeds and alternative hosts, in an overall integrated management package, helped to control viral diseases. Lloyd et al. (1974) were able to eliminate banana viruses by use of virus-free planting material obtained by thermal therapy and tissue culture. Other effective methods reported are debris removal from the field, disinfection of support trellises before re-use, crop quarantine measures executed for incoming seeds, and crop rotation (AVRDC, 1985; Green and Kim, 1991); use of skimmed milk foliar sprays (Black et al., 1991; Green et al., 1991); application of antiviral agents like cytovirin (Simmons 1959), and treatment of tomato seed with trisodium phosphate for 1 hour (Broadbent, 1965 and 1976). 2.1.4.1.2 Cultural Control There are a number of cultural control measures, such as close plant spacing to compensate for yield losses through diseased plants, use of mulching and intercropping with nitrogen fixing cover crops like Lablab purpureus L. (Cohen et al., 1974). Greer and 42
Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases Dole (2003) reported that aluminium foil and mulches are effective in repelling insect pests, and that black plastic mulches improve crop yield better than bare ground. Mutwiwa et al. (2005) found that ultra-violet light absorbing plastics used as mulches or green house roofing material repel whitefly pests when low UV-intensity plastics are used. Other cultural options include timely planting, intercropping, and use of barrier hedges (Simons et al., 1980). These methods effectively reduce tomato yield losses, virus incidence and spread, even though they are simple and common. 2.1.4.2 Cross-protection Cross-protection is the method of controlling virus disease by using a mild virus strain to immunize otherwise healthy plants against a severe virus strain (Matthews, 1991). The method has been used against ToMV and TSWV. Avgelis (1987) used a mild strain (MX lV – l) of ToMV to cross-protect ToMV-susceptible tomato cultivars Earlypak and Dombo C2 VF2 in Crete, leading to 14% yield improvement. He expected higher yields, and as such attributed low increase in yield to higher than expected temperatures, which could have reduced the effectiveness of mild strains. Another example of cross-protection is when Min-Wang and Gonsalves (1992) and Cho et al. (1989) used nitrous acid to induce mutation of TSWV to mutant R27G, which was put in tomato in order to control severe TSWV strains from Hawai and Oklahoma. This method requires more sophisticated facilities and careful management of the outcome, which could otherwise result into more dangerous strains. With limited resources in the south, one would to be very careful to recommend use of such a technology, even though it could be required in some instances. 2.1.4.3 Host Plant Resistance Use of resistant crop varieties is the most convenient and cost-effective control measure of all (Hall, 1980; Gajos, 1981; Kumar and Irulappan, 1992; Nono-Womdim, 1993; Rubio et al., 2003; Yang et al., 2004; de Castro et al., 2005). For example, planting of 43
- Page 16 and 17: xiii 13 Beide genhomologie percenta
- Page 18 and 19: xv 15 dit onderzoek geven ook aan d
- Page 20 and 21: LIST OF TABLES xvii 17 Table Page 1
- Page 22 and 23: ACRONYMS AND ABBREVIATIONS xix 19 A
- Page 24 and 25: Abbreviation Word in full PCR Polym
- Page 26 and 27: Incidence, distribution and charact
- Page 28 and 29: Incidence, distribution and charact
- Page 30 and 31: Incidence, distribution and charact
- Page 32 and 33: Incidence, distribution and charact
- Page 34 and 35: Incidence, distribution and charact
- Page 36 and 37: Table 2.1: The orthography of some
- Page 38 and 39: Incidence, distribution and charact
- Page 40 and 41: Incidence, distribution and charact
- Page 42 and 43: Incidence, distribution and charact
- Page 44 and 45: Incidence, distribution and charact
- Page 46 and 47: Incidence, distribution and charact
- Page 48 and 49: Incidence, distribution and charact
- Page 50 and 51: Incidence, distribution and charact
- Page 52 and 53: Incidence, distribution and charact
- Page 54 and 55: Incidence, distribution and charact
- Page 56 and 57: Incidence, distribution and charact
- Page 58 and 59: Incidence, distribution and charact
- Page 60 and 61: Incidence, distribution and charact
- Page 62 and 63: Incidence, distribution and charact
- Page 64 and 65: Incidence, distribution and charact
- Page 68 and 69: Incidence, distribution and charact
- Page 70 and 71: Incidence, distribution and charact
- Page 72 and 73: Incidence, distribution and charact
- Page 74 and 75: Incidence, distribution and charact
- Page 76 and 77: Incidence, distribution and charact
- Page 78 and 79: A B Incidence, distribution and cha
- Page 80 and 81: A B Incidence, distribution and cha
- Page 82 and 83: A Incidence, distribution and chara
- Page 84 and 85: Incidence, distribution and charact
- Page 86 and 87: Incidence, distribution and charact
- Page 88 and 89: Incidence, distribution and charact
- Page 90 and 91: Incidence, distribution and charact
- Page 92 and 93: Incidence, distribution and charact
- Page 94 and 95: Incidence, distribution and charact
- Page 96 and 97: Incidence, distribution and charact
- Page 98 and 99: Incidence, distribution and charact
- Page 100 and 101: Incidence, distribution and charact
- Page 102 and 103: Incidence, distribution and charact
- Page 104 and 105: Incidence, distribution and charact
- Page 106 and 107: Isolate IG1 Incidence, distribution
- Page 108 and 109: Incidence, distribution and charact
- Page 110 and 111: Incidence, distribution and charact
- Page 112 and 113: 1600 500 700 200 Incidence, distrib
- Page 114 and 115: Incidence, distribution and charact
<strong>Incidence</strong>, distribution <strong>and</strong> characteristics <strong>of</strong> major tomato leaf curl <strong>and</strong> mosaic virus diseases<br />
Eretmocerus spp., as well as predators like lacewing <strong>and</strong> coccinelids (Zalon et al., 2003).<br />
However, concentrating on vector control is not always very effective since some vectors<br />
transmit viruses in a persistent manner (Green, 1991; Gianessi et al., 2003), so that even<br />
at very low densities, some vectors are effective in transmitting viruses. Moreover,<br />
transmission <strong>of</strong> non-persistent viruses takes place quickly <strong>and</strong> readily, <strong>and</strong> is <strong>of</strong>ten<br />
performed by vectors that visit, but do not establish their colonies on the visited crop.<br />
According to Perring et al. (1999), no one method <strong>of</strong> control is likely to keep crops free<br />
<strong>of</strong> vectors <strong>and</strong> virus infections.<br />
2.1.4.1.1 Crop Sanitation<br />
A number <strong>of</strong> cultural practices have been reported to reduce incidence <strong>and</strong> severity <strong>of</strong><br />
viral diseases. These include elimination <strong>of</strong> inoculum sources by removal <strong>of</strong> infected<br />
plants <strong>and</strong> alternative hosts. Chan <strong>and</strong> Jeger (1994), while working with an analytical<br />
model <strong>of</strong> plant virus disease dynamics with uprooting <strong>and</strong> replanting, found that in<br />
sparsely planted crops intensive uprooting only was able to eradicate virus diseases. Cho<br />
et al. (1989) reported that removing TSWV-infected crop plants, weeds <strong>and</strong> alternative<br />
hosts, in an overall integrated management package, helped to control viral diseases.<br />
Lloyd et al. (1974) were able to eliminate banana viruses by use <strong>of</strong> virus-free planting<br />
material obtained by thermal therapy <strong>and</strong> tissue culture. Other effective methods reported<br />
are debris removal from the field, disinfection <strong>of</strong> support trellises before re-use, crop<br />
quarantine measures executed for incoming seeds, <strong>and</strong> crop rotation (AVRDC, 1985;<br />
Green <strong>and</strong> Kim, 1991); use <strong>of</strong> skimmed milk foliar sprays (Black et al., 1991; Green et<br />
al., 1991); application <strong>of</strong> antiviral agents like cytovirin (Simmons 1959), <strong>and</strong> treatment <strong>of</strong><br />
tomato seed with trisodium phosphate for 1 hour (Broadbent, 1965 <strong>and</strong> 1976).<br />
2.1.4.1.2 Cultural Control<br />
There are a number <strong>of</strong> cultural control measures, such as close plant spacing to<br />
compensate for yield losses through diseased plants, use <strong>of</strong> mulching <strong>and</strong> intercropping<br />
with nitrogen fixing cover crops like Lablab purpureus L. (Cohen et al., 1974). Greer <strong>and</strong><br />
42