Incidence, Distribution and Characteristics of Major Tomato Leaf ...

More documents

Recommendations

Info

Annex 6: Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases 194 Figure A.6.1: CLUSTAL W (1.82) nucleotide multiple sequence alignment of (a) 521bp of coat protein region, (IR, V1, V2 ORFs), and (b) 482bp of replicative gene (C1 ORF) for tomato leaf curl virus (ToLCV) from Uganda with geminiviruses OkLCV-EG, CoLCV-SD, ToLCV-Ma, TbLCZWV, TbLCZWmd, ATLCV-Tz, EACMV-UG, AEV- NP, TYLCV-Is, TYLCV-JR, TYLCV-OM, TLCV-SD, ToLCV-IN, ToLCV-TW, TbLCV-Ph, SCLV-JR, ToLCV-PH, TCSV-SA, ToLCV-AU and ChaMV-Ng for outgroup rooting (-) indicates no consensus, while areas of maximum consensus are marked with asterisks Fig.A.6.1a) ToLCV-Ugrev TbLCZbwV -------------------------------TATTGGGCCTCCAA--GGCCGCGCAGCGG 27 TbLCZbwV-md -------------------------------TAAACGCTTTTGTATTAGCCGCGCAGCGG 29 ATLCV-Tz -------------------------------TCATGGGTCTTAAT--GGCCGCGCAGCGG 27 ChaMV-Ng -------------------------------TACTCGGCCTCCAC--GGCCGCGCAGCGG 27 AEV-NP -------------------------------TTTGAGGCCTCAGA--GGCCGCGCAGCGG 27 OkLCV-EG -------------------------------TAATCGGTCTACTT--GGCCGCGCAGCGG 27 CoLCV-SD -------------------------------TAATCGGTCTACTT--GGCCGCGCAGCGG 27 ToLCV-Ma -------------------------------TATTGGGTCTCCAA--GGCCGCGCAGCGG 27 ToLCV-UGrev -------------------------------TGTGGGATCCACTGTTAA---ATGAGTTC 26 EACMV-MW -------------------------------TGTGGGATCCATTGTTGA---ATGAGTTC 26 TYLCV-Is/PT -------------------------------TGTGGGATCCACTTCTAA---ATGAATTT 26 TYLCV-JR/Szka -------------------------------TGTGGGATCCACTTCTAA---ATGAATTT 26 TYLCV-OM -------------------------------TGTGGGACCCACTTCTAA---ATGAATTT 26 TLCV-SD/Gez -------------------------------TGTGGGATCCACTTCTAA---ATGAATTT 26 ToLCV-IN/Bg -------------------------------TGTGGGATCCACTTACAA---ATGATTTT 26 ToLCV-TW -------------------------------TGTGGGATCCACTTTTAA---ATGATTTT 26 * * * TbLCZbwV CACCACACACATTCTCA---GAGACCCACCCTTCAAGTTCCTCAGGGACTTGATCAAAAG 84 TbLCZbwV-md CACCACACACATTCTCA---GAGACCCACTCTTCAAGTCCCTCAGGGACTTGATCAAAAG 86 ATLCV-Tz TAGTTAGAACATTCTGT---GCCGCCCACTCTTCAAGTTCCTCTGGAACTTGATCGAAAG 84 ChaMV-Ng CATCCATCACGTTCGCC---GATGCCCATCGTTGAAGTTCATCAGGAACCTGAGTGAAGG 84 AEV-NP CATTCATGACATTTTCT---GAGACCCATTCTTCAAGTTCTTCAGGAACTTGATCAAAAG 84 OkLCV-EG CCTCAACGACGTTCTCC---GCCGCCCATTCTTCAAGTTCTTCGGGAACTTGGTCGAACG 84 CoLCV-SD CATCAACGACGTTCTCC---GCCGCCCATTCTTCAAGTTCTTCTGGAACTTGGTCGAACG 84 ToLCV-Ma CATCCCTGACGTTCTTA---GAAACCCACTCTTCAAGTTCTTCGGGAACTTGATCGAAAG 84 TbLCV-Ch CATGCCTTACATTATCAA--GCAGCCCACTCTTCAAGTTCTTCGGGAACTTGATCAAATG 85 ToLCV-UGrev CCAGACTC-TGTTC------ATGGGT-TTCG---TTGCAT-----GCTTGCTATAAAATA 70 EACMV-MW CCCGATTC-TGTGC------ACGGTT-TTCG---CTGTAT-----GCTTGCTATTAAATA 70 TYLCV-Is/PT CCTGAATC-TGTTC------ACGGAT-TTCG---TTGTAT-----GTTAGCTATTAAATA 70 TYLCV-JR/Szka CCTGAATC-TGTTC------ACGGAT-TTCG---TTGTAT-----GTTAGCTATTAAATA 70 TYLCV-OM CCTGAATC-TGTTC------ACGGAT-TTCG---TTGTAT-----GTTAGCTATTAAATA 70 TLCV-SD/Gez CCTGAATC-TGTTC------ATGGAT-TTCG---TTGTAT-----GTTAGCTATTAAATA 70 ToLCV-IN/Bg CCCGAAAC-CGTAC------ACGGAT-TCAG---GTGTAT-----GCTTGCTATAAAATA 70 ToLCV-TW CCGGAGTC-TGTG--C----ATGGTC-TACG---GTGTAT-----GCTTGCCATTAAGTA 70 * * * TbLCZbwV A--AGAAGAAGAGAAA-GGAGAAATATAAGGCTCTGGGGGAGCCTGGAAAATC------- 134 TbLCZbwV-md A--AGAAGAAGAGAAA-GGAGAAATATAAGGCTCTGGGGGAGCCTGGAAAATC------- 136 ATLCV-Tz A--AGAACATAAGAAA-GGAGAAACATAATCCTCCAACGGAGGTGTAAAAATC------- 134 ChaMV-Ng A--GGAAGCACAGAAG-GGAGAAACATAAACCTCCGGAGGAGCTTGGAAGATC------- 134 AEV-NP A--AGAAGATAAAAAA-GGCGAAACATAAACCTCCAACGGAGGAGTAAAAATC------- 134 OkLCV-EG A--AGAAGACAAAAAA-GGAGAAACATAAGGAGCTGGAGGCTCCTGAAAAATC------- 134 CoLCV-SD A--AGAAGACAAAAAA-GGAGAAACATAAGGAGCTGGAGGCTCCTGAAAAATC------- 134
Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases 195 Fig.A.6.1a) continued ToLCV-Ma A--AGAAGAAAGAAAA-GGACAAACAAAAACCTCTACAGGAGGTGCAAAAATC------- 134 TbLCV-Ch A--TGAAGATAAAAAA-GGAGAAACAAAA-CCTCTAAAGGAGGTGCAAAAATC------- 134 ToLCV-UGrev TTTGCAGGCTATTGAG-TCCA---CTTACGAGCC-CAATACTTTGGGCC--ACGATTTAA 123 EACMV-MW TTTGCAGGCCTTGGAG-GAAA---CCTACGAGCC-CAATACTTTGGGCC--ACGATCTAG 123 TYLCV-Is/PT TTTGCAGTCCGTTGAG-GAAA---CTTACGAGCC-CAATACATTGGGCC--ACGATTTAA 123 TYLCV-JR/Szka TTTGCAGTCCGTTGAG-GAAA---CTTACGAGCC-CAATACATTGGGCC--ACGATTTAA 123 TYLCV-OM TTTGCAGTCCGTTGAG-GAAA---CTTACGAGCC-CAATACATTGGGCC--ACGATTTAA 123 TLCV-SD/Gez TTTGCAGGCCGTTGAG-GAAA---CTTACGAGCC-CAATACATTGGGCC--ACGATTTAA 123 ToLCV-IN/Bg CTTGCAGTTGGTAGAA-AATA---CGTATTCCCC-CGATTCTTTGGGAT--ACGATCTAA 123 ToLCV-TW TCTACATTTTGTAGAA-AATA---CATATTCCCCGGAT-ACTTTGGGTT--ACGATCTCA 123 * * ***** * TbLCZbwV -------------------------------------------------CTA--TCTAAA 143 TbLCZbwV-md -------------------------------------------------CTA--TCTAAA 145 ATLCV-Tz -------------------------------------------------CTA--TCTAAA 143 ChaMV-Ng -------------------------------------------------TTA--TCCAAA 143 AEV-NP -------------------------------------------------CTA--TCTAAA 143 OkLCV-EG -------------------------------------------------CTC--TCTAAA 143 CoLCV-SD -------------------------------------------------CTC--TCTAAA 143 ToLCV-Ma -------------------------------------------------CTA--TCTAAA 143 TbLCV-Ch -------------------------------------------------CTA--TCTAAA 143 ToLCV-UGrev TTCGA-------GAT---------------------------------CTCA--TTTGTG 141 EACMV-MW TCCGT-------GAT---------------------------------CTCA--TCTGTG 141 TYLCV-Is/PT TTAGG-------GAT---------------------------------CTTA--TATCTG 141 TYLCV-JR/Szka TTAGG-------GAT---------------------------------CTTA--TATCTG 141 TYLCV-OM TTAGG-------GAT---------------------------------CTTA--TATCTG 141 TLCV-SD/Gez TTAGG-------GAT---------------------------------CTTA--TATCTG 141 ToLCV-IN/Bg TACGT-------GAT---------------------------------TTAA--TTTCTG 141 ToLCV-TW TACGT-------GAT---------------------------------TTGA--TTTTAG 141 * * TbLCZbwV TTAGT-----CTTTAAATTATGATACTGAAAAATAAAATCTTTAGGGAGTTTTTCCCTAA 198 TbLCZbwV-md TTAGT-----CTTTAAATTATGATACTGAAAAATAAAATCTTTAGGGAGTTTTTCCCTAA 200 ATLCV-Tz TTACA-----TTTTAAATTATGATACTGAAAAATGAAATCTTTAGGGAGTTTCTCCCTAA 198 ChaMV-Ng TTAGC-----CTTGATATTATGATATTGAAATATATAATCTTTGGGGAGTTTCTCCTTAA 198 AEV-NP TTAGA-----ATTTAAATTATGATATTGAAAAATAAATTTTTCTGGGAGTTTCTCCTTTA 198 OkLCV-EG TTACT-----ATTTAAATTATGAAATTGTAAAACAAAATCTTTGGGAGCTAACTCCCTTA 198 CoLCV-SD TTACT-----ATTTAAATTATGAAATTGTAAAACAAAATCTTTGGGAGCTAACTCCCTTA 198 ToLCV-Ma TTAGA-----ATTTAAATTATGAAATTGTAAAACAAAATCTTTAGGAGCTAACTCCTTAA 198 TbLCV-Ch TTAGC-----ATTTAAATTATGAAATTGTAAAACAAAATCTTTAGGAGCTAACTCCTTAA 198 ToLCV-UGrev TCGTT---AGAGCCAGAGATTATGTCGAAGCGACCCGG---AGATATAATAATTTCAACG 195 EACMV-MW TTATC---CGAGCCCGTGATTATGTCGAAGCGACCCGC---AGATATAATCATTTCCACT 195 TYLCV-Is/PT TTGTA---AGGGCCCGTGACTATGTCGAAGCGACCAGG---CGATATAATCATTTCCACG 195 TYLCV-JR/Szka TTGTA---AGGGCCCGTGACTATGTCGAAGCGACCAGG---CGATATAATCATTTCCACG 195 TYLCV-OM TTGTA---AGGGCCCGTGACTATGTCGAAGCGACCAGG---CGATATAATCATTTCCACG 195 TLCV-SD/Gez TTGTA---AGGGCACGTGACTATGTCGAAGCGACCAGG---CGATATAATCATTTCCACG 195 ToLCV-IN/Bg TCGTC---AGGGC---------------------------------TAAA---------- 155 ToLCV-TW TTATC---CGTGCTAGAGATTATGTCGAAGCGACCCGC---CGATATAGTC--------- 186 * TbLCZbwV TAATAGCCATAGCTGCTTCAGCGGAACCTGCGTTTAAAGCGTCGGCGCATGCGTCGTTAG 258 TbLCZbwV-md TAATAGCCATAGCTGCTTCAGCGGAACCTGCGTTTAAAGCGTCTGCGCATGCGTCGTTAG 260 ATLCV-Tz TAATAGCCAGAGCGGCTTCAGCGGATCCTGCGTTTAATGCCTCGGCGCATGCGTCGTTAG 258 ChaMV-Ng TAATAGCCAACGCAGCTTCAGCGGAACCTGCGTTTAACGCCTCGGCGGCTGCGTCATTAG 258 AEV-NP TTATAGCCATTGCAGCTTCTTTAGAACCTGCATTTAGGGCTTCTGCAGCAGCATCATTAG 258 OkLCV-EG TAACCCTAAGAGCCTCTGCCTTACTTCCTGCGTTAAGCGCTGCGGCGTAAGCGTCATTGG 258 CoLCV-SD TAACCCTAAGAGCCTCTGCCTTACTTCCTGCGTTAAGCGCTGCGGCGTAAGCGTCATTGG 258 ToLCV-Ma TTACATTAAGAGCCTCTGACTTACTGCCGCTGTTAAGTGCCTGTGCGTAAGCGTCATTGG 258 TbLCV-Ch TTACATTAAGAGCCTCTGACTTACTGCCGCTGTTAAGTGCCTGGGCGTAAGCGTCATTGG 258 ToLCV-UGrev C-CCGCCTCG-AAGGTTCG---TCGAAGGTTG-----AACTTCGACAGC----CCGTACA 241 EACMV-MW C-CCGTCTCG-AAGGTGCG---TCGAAGGCTG-----AACTTCGACAGC----CCGTTCA 241 TYLCV-Is/PT C-CCGCCTCG-AAGGTTCG---CCGAAGGCTG-----AACTTCGACAGC----CCATACA 241 TYLCV-JR/Szka C-CCGCCTCG-AAGGTTCG---CCGAAGGCTG-----AACTTCGACAGC----CCATACA 241 TYLCV-OM C-CCGTCTCG-AAGGTTCG---CCGAAGGCTG-----AACTTCGACAGC----CCATACA 241 TLCV-SD/Gez C-CCGTCTCG-AAGGTTCG---CCGAAGGCTG-----AACTTCGACAGC----CCATACA 241 ToLCV-IN/Bg --CCGACTT--------------------CTG-----AGCTCAATCAGC----CCATACA 184 ToLCV-TW -----------A---------------------------TTTCCACTCA----CCGTGTA 204 * *
Page 1:
Incidence, Distribution and Charact
Page 4 and 5:
Charles SSEKYEWA Incidence, Distrib
Page 6 and 7:
CONTENTS 1 CHAPTER 1...............
Page 8 and 9:
ACKNOWLEDGEMENT The following perso
Page 10 and 11:
vii 7 mosaic virus (CMV), Alfalfa m
Page 12 and 13:
ix 9 Consequently, our findings con
Page 14 and 15:
SAMENVATTING Tomaat is een economis
Page 16 and 17:
xiii 13 Beide genhomologie percenta
Page 18 and 19:
xv 15 dit onderzoek geven ook aan d
Page 20 and 21:
LIST OF TABLES xvii 17 Table Page 1
Page 22 and 23:
ACRONYMS AND ABBREVIATIONS xix 19 A
Page 24 and 25:
Abbreviation Word in full PCR Polym
Page 26 and 27:
Incidence, distribution and charact
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Table 2.1: The orthography of some
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
A B Incidence, distribution and cha
Page 80 and 81:
A B Incidence, distribution and cha
Page 82 and 83:
A Incidence, distribution and chara
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Isolate IG1 Incidence, distribution
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
1600 500 700 200 Incidence, distrib
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
1200 1200 (A) IG1 (ToLCV-UG) PAL1v
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
78 Incidence, distribution and char
Page 134 and 135:
87 87 Incidence, distribution and c
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Page 142 and 143:
Page 144 and 145:
A A C D Incidence, distribution and
Page 146 and 147:
Opaque and not smeared Translucent
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Rainfall (cm) Temp. Cº Mean Whitef
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Page 168 and 169: Incidence, distribution and charact
Page 198 and 199: 8 ANNEXES Incidence, distribution a
Page 202 and 203: Table A.1.1 continued Incidence, di
Page 216 and 217: Annex 5: Incidence, distribution an
Page 232: Books Incidence, distribution and c
show all

Incidence, Distribution and Characteristics of Major Tomato Leaf ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?