Incidence, Distribution and Characteristics of Major Tomato Leaf ...

LIST OF TABLES
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Table Page
1.1 Nutrient Contents of Vegetables (AVRDC, 2003) 2
2.1 The orthography of some tomato virus taxon names 12
3.1 Agro-climatic zones of Uganda 31
3.2 Some samples collected and subjected to molecular tests 63
3.3 Primers used in PCR reactions 65
3.4 Cultivated crops and weeds bearing leaf curl and mosaic symptoms
tested for TYLCV (sensu lato)
72
3.5 Weeds tested for ToLCV-UG begomovirus 73
3.6 Percentage number of samples showing characteristic symptoms of
tomato viruses per district in 1997
74
3.7 Grouping of samples and incidence of viruses detected in the seven
districts surveyed in (a)1996-1997, and (b) 1998.
78
3.8 Frequency of various combinations as found in tomato samples 79
3.9 Reaction of dot-blotted tomato geminivirus samples to the general and
specific probes
83
3.10 Legends for DNA Hybridisation membranes 84
3.11 Summary of DNA hybridization and PCR results also indicating cloned
and sequenced samples
86
3.12 ToLCV-UG specific primers prepared during this study 89
3.12 Samples of alternative hosts positive for tomato yellow leaf curl viruses
in Uganda
4.1 List of sequences used in the phylogenetic analysis 105
4.2 Clustal W (1.82) multiple sequence alignment of ToLCV-UGf (482bp) 107
4.3 Clustal W (1.82) multiple sequence alignment of ToLCV-UGr (521bp) 107
4.4 TYLCV-UG (360bp) segment of the Intergenic Region, Clustal W (1.82) 107
5.1 Mean total whitefly counts for blue and yellow traps, and TYLCVinfected
plants throughout trial I.
124
5.2 Ranked Mean number of whiteflies trapped by Kubwa trap per treatment,
in trial I.
130
5.3 Ranked mean weekly number of whiteflies trapped with Kubwa trap and
the related TYLCV incidence, in trial II
130
5.4 Ranked mean Whitefly population on tomato in relation to ranked mean
number of TYLCV infected tomato plants, in trial III.
131
5.5 Mean number of whiteflies trapped by Kubwa trap per treatment in trials
I to III
133
5.6 Mean distance from initial infection to secondary infected plants 137
5.7 Infection rate for the different experiment plots for the period February
1999 to February 2000
5.8 Mean number of fruits, total yield and marketable yield (kgs) per 90 m²,
in trial I, II and III.
90
137
142