Incidence, Distribution and Characteristics of Major Tomato Leaf ...

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Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases Table 3.11: Summary of DNA hybridisation and PCR results, also showing cloned and sequenced samples Samples Hybridisation Primer PAv 494 and Pac 1048 PTYCRv21 and PTYCRc287 Polymerase Chain Reaction (PCR) Cloning and Sequencing TYLCV-EG (TYLCV-Is) PTYCRv 1499 and PTYCRc 2196 PTYCRv 466 and PTYCRc 1814 TLCV-UG Specific primers PTLCV-UGrep2r and PTLC-UGcp1f TYLCV CP TYLCV RL1 - +¹ -² - + 0 - 0 0 0 RL2 - + - ++ +++ 0 - 0 0 0 RL5 ++ + + ++ +++ - - + 0 + K2 + - 0 0³ 0 0 0 0 0 0 IG1 +++ + +++¹ 0 ++¹ 0 ++ - 0 + KN - - 0 0 0 0 0 0 0 0 MB9 + + ++ 0 + 0 - 0 0 0 K3 ++ ++ - +++ +++ - + 0 0 0 IG2 - - 0 0 0 0 0 0 0 0 MB8 - + ++ +++ +++ 0 - 0 0 0 MB7 - + 0 0 0 0 0 0 0 0 MB2 - - 0 0 0 0 0 0 0 0 IG3 + - 0 0 0 0 0 0 0 0 MB6 - - 0 0 0 0 0 0 0 0 ISOPOT ++ + +++ +++ +++ 0 - 0 0 0 CVMV + 0 - 0 +++ 0 - 0 0 0 CVMVmxd + 0 ++ 0 + 0 0 0 0 0 (¹+,…, +++) = intensity of positive reaction to geminiviruses’ test; (-)² = negative reaction to geminiviruses’ test; ³ (0) stand for samples that were not included in that particular experiment. 86 TLVC-UG
Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases Figure 3.12 Reaction of tomato virus samples to degenerate primers PAL1 v 1978 and PAR1c 715: (A and B) 1% agarose in TBE buffer, at 97 volts; (C) reaction of tomato samples to coat protein degenerate primers PAR Av 494 and PAR Ac 1048: 1% agarose in TBE buffer, at 97volts; (D and E) agarose gel (1%) electrophoresis, reaction of PCR product DNA for selected samples where primers PTYCRv 21 and PTY IR c287 were used; (F and G) agarose gel (1%) electrophoresis photo showing reaction of PCR product DNA for selected samples using primers PTYC2v 1499 and PTYAL1c 2196; (H) agarose gel (1%) electrophoresis results photo showing reaction of PCR product DNA using primers PTYCrc 1814 and PTYV2v466; (I) agarose gel (1%) electrophoresis results, showing reaction of PCR product DNA for selected samples with primers PTLCVUGrep2r and cp1f NB: Photos were labelled according to the 1kb Marker Ladder from Biotechnology Research Laboratory (BRL), Wisconsin-Madison, USA 87
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Incidence, Distribution and Charact
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Charles SSEKYEWA Incidence, Distrib
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CONTENTS 1 CHAPTER 1...............
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ACKNOWLEDGEMENT The following perso
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vii 7 mosaic virus (CMV), Alfalfa m
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ix 9 Consequently, our findings con
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SAMENVATTING Tomaat is een economis
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xiii 13 Beide genhomologie percenta
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xv 15 dit onderzoek geven ook aan d
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LIST OF TABLES xvii 17 Table Page 1
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ACRONYMS AND ABBREVIATIONS xix 19 A
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Abbreviation Word in full PCR Polym
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Incidence, distribution and charact
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Table 2.1: The orthography of some
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8 ANNEXES Incidence, distribution a
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Table A.1.1 continued Incidence, di
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Annex 5: Incidence, distribution an
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Books Incidence, distribution and c
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