Incidence, Distribution and Characteristics of Major Tomato Leaf ...

Incidence, distribution and characteristics of major tomato leaf curl and mosaic virus diseases
Table 3.10: Legends for DNA hybridisation membranes shown in Figure 3.11: (a) Refers
to squash-blotted membranes A and D; (b) refers to dot-blotted membranes E and F; (c)
refers to dot-blotted membranes B and C.
a) Legend for squash-blotted membranes A (general probe) and D (specific probe)
A B C D E F G H I
1 MB1 MB2 MB3 MB4 ISO 2 ISO 3 ISO 4 ISO 5 ISO 6
28/5/98 28/5/98 28/5/98 28/5/98
2 ISO 6 ISO 7 ISO 8 ISO 9 ISO 10 ISO 11 ISO 12 ISO 13 ISO 14
3 ISO 15 ISO 16 ISO 17 ISO 18 ISO 19 ISO 20 ISO 21 ISO 22 ISO 23
4 ISO 24 ISO 25 ISO 26 ISO 27 ISO 28 ISO 29 ISO 30 ISO 31 Healthy
5 IG 10/ IG 7/ MB 4/ MB 20/ MB 2/ MB 5/ MB 3/ MB 10/ MB 21/
22/7 22/7 22/7 22/7 22/7 22/7 22/7 22/7 22/7
6 RL 5 IG 1 IG 2 IG 2 IG 2 K2 TYLCV BGMV -ve
3/9/98 3/9/98 3/9/98 3/9/98 3/9/98
b) Legend for dot-blotted membranes E and F (specific probe)
A B C D E F
1 IG1 IG2 RL5 K2 K3 Healthy
2 CVMV K1 RL1 RL2 ISOPOT Diseased
3 MB8 CVMVmx MB6 MB9 MB7 Healthy
c) Legend for dot-blotted membranes B and C (mixed probe)
A B C D E F G H I J K
1 RL1 RL1 RL2 RL2 RL5 RL5 K2 K2 IGI IG1 TYLCV
2 KN KN MB9 MB9 K3 K3 IG2 IG2 MB8 MB8 BGMV
3 MB7 MB7 MB2 MB2 IG3 IG3 MB6 MB6 ISOPOT ISOPOT Healthy
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