P9-7 Upala za ispitivane parametre. Sva tri parametra bila su značajno povišena u trudnica koje su prijevremeno rodile u odnosu na kontrolnu skupinu s još izraženijim razlikama koncentracija u prisutnosti konatalne infekcije i PVL. Naši rezultati pokazuju kako IL-6, IL-1 beta i CRP daju vrijednu informaciju o riziku od infekcijom uzrokovanog prijevremenog poroda, fetalne infekcije i nastanka PVL još prije poroda. E-mail: sasa.kralik-oguic@zg.htnet.hr P9-8 Koncentracija TNF-alfa, CXCL8, velikog ET- 1 i hsCRP kod zdravih nepušača, pušača i bolesnika s KOPB Marević S 1 , Petrik J 2 , Vrkić N 3 , Popović-Grle S 4 , Čepelak I 1 1Klinika za infektivne bolesti Dr. Fran Mihaljević, Zagreb, Hrvatska 2Farmaceutsko-biokemijski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 3KB Sestre milosrdnice, Zagreb, Hrvatska 4Klinika za plućne bolesti Jordanovac, Zagreb, Hrvatska Kronična opstrukcijska plućna bolest (KOPB) je progresivna kronična upalna bolest obilježena smanjenjem protoka zraka kroz dišne putove koje nije potpuno reverzibilno. Premda patološki mehanizmi razvoja KOPB još nisu potpuno jasni, poznato je da različite stanice u plućima oslobađaju brojne upalne medijatore. TNF-alfa (čimbenik tumorske nekroze-alfa), oslobađanje kojega izaziva duhanski dim, ključni je proupalni citokin u patogenezi KOPB. TNF-alfa aktivira transkripcijske čimbenike i potiče transkripciju gena za citokine, kemokine, proteaze i endoteline. CXCL8 (interleukin-8) je kemokin koji privlači neutrofi le u upalno područje. ET-1 (endotelin-1) i veliki ET-1 doprinose plućnoj vazokonstrikciji i plućnoj hipertenziji u KOPB. hsCRP (visoko osjetljivi C-reaktivni protein) je protein akutne faze povišen u upalnim bolestima. Cilj istraživanja bio je ispitati koncentracije i međusobnu korelaciju TNF-alfa, CXCL8, velikog ET-1 i hsCRP kod zdravih nepušača, zdravih pušača i bolesnika s KOPB. Koncentracije TNF-alfa, CXCL8 i velikog ET-1 određene su u plazmi kod zdravih nepušača (n=23, kontrolna skupina), zdravih pušača (n=30) i bolesnika s KOPB (n=30) gotovim ELISA test paketom, a koncentracija hsCRP u serumu nefelometrijskom metodom. Koncentracija hsCRP kod bolesnika s KOPB bila je značajno povećana u odnosu na vrijednosti kontrolne skupine (p=0,0004), dok koncentracije TNF-alfa (p=0,0788), CXCL8 (p=0,4839) i velikog ET-1 (p=0,3641) able prediction of perinatal infection and PVL. All three parameters were signifi cantly higher in patients with premature delivery than in control patients with term delivery, with even more pronounced diff erences in the rate of perinatal infection and PVL. Our results indicate that maternal IL-6, IL-1 beta and CRP levels provide information on the risk of infection-complicated preterm labor, fetal infection and PVL before birth. E-mail: sasa.kralik-oguic@zg.htnet.hr P9-8 Infl ammation Concentrations of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, smokers and COPD patients Marević S 1 , Petrik J 2 , Vrkić N 3 , Popović-Grle S 4 , Čepelak I 1 1Dr. Fran Mihaljević University Hospital for Infectious Diseases, Zagreb, Croatia 2School of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia 3Sestre milosrdnice University Hospital, Zagreb, Croatia 4Jordanovac University Hospital for Lung Diseases, Zagreb, Croatia COPD (chronic obstructive pulmonary disease) is a chronic progressive infl ammatory disease characterized by limitations in lung airfl ow that is not fully reversible. Although the pathophysiology of COPD is not understood completely, it is well known that various cells in the lungs are releasing infl ammatory mediators. Tobacco smoke induces the release of a proinfl ammatory cytokine TNFalpha (tumor necrosis factor-alpha) that might play a key role in COPD. TNF-alpha activates certain transcription factors and switches on the transcription of genes for cytokines, chemokines, proteases and endothelins. CXCL8 (interleukin-8) is a chemokine that attracts neutrophils to the infl ammatory site. ET-1 (endothelin-1) and big ET-1 contribute to pulmonary vasoconstriction and pulmonary hypertension in COPD patients. hsCRP (highly sensitivity C-reactive protein) is an acute phase protein elevated in infl ammatory diseases. The aim of the study was to investigate the concentrations and correlation of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, healthy smokers and patients with COPD. The concentration of TNF-alpha and CXCL8 in serum and big ET-1 in plasma were measured with an ELISA test in healthy nonsmokers (n=23, control group), healthy smokers (n=30) and COPD patients (n=30). hsCRP was measured in serum nephelometrically. The concentration of hsCRP was signifi cantly higher in <strong>Biochemia</strong> <strong>Medica</strong> 2006;16(Suppl 1):S1–S268 S141
P9-8 Upala nisu bile značajno promijenjene. Koncentracije svih analita u uzorcima pušača također se nisu značajno razlikovale od vrijednosti u kontrolnoj skupini. Spearmanov koefi cijent korelacije za koncentracije TNF-alfa i CXCL8 iznosio je r= 0,638 (p
Change language