Posterske teme Poster topics - Biochemia Medica
Posterske teme Poster topics - Biochemia Medica
Posterske teme Poster topics - Biochemia Medica
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P23-1 Ostalo<br />
sidacijskim stresom i štetnim reakcijama kao što su peroksidacija<br />
višestruko nezasićenih masnih kiselina i smanjena<br />
aktivnost antioksidacijskih enzima. Mehanizam kojim<br />
je Fe uključen u započinjanje ili poticanje oksidacijskog<br />
oštećenja nije sasvim jasan. Cilj ovoga istraživanja bio je<br />
ispitivanje učinka velikih količina Fe na antioksidacijski<br />
status miševa čija je hrana obogaćena kukuruznim uljem.<br />
Mužjaci miševa Balb/c hranjeni su 3 tjedna standardnom<br />
prehranom obogaćenom s 5% kukuruznog ulja i s optimalnom<br />
količinom Fe (skupina FCO), odnosno obogaćenom s<br />
1% karbonilnog Fe (skupina FCOFe). Kontrolna skupina je<br />
hranjena standardnom prehranom. Količina Fe, bakra (Cu)<br />
i cinka (Zn) u tkivu jetre određeni su induktivno spregnutom<br />
plazma spektrometrijom. Aktivnosti Cu/Zn superoksid<br />
dismutaze (CuZnSOD) i glutation peroksidaze (GPx)<br />
određene su spektrofotometrijski u nadsloju homogenata<br />
jetre. Lipidna peroksidacija određena je mjerenjem<br />
količine reaktivnih spojeva s tiobarbiturnom kiselinom<br />
(TBARS). Prehrana s velikim udjelom Fe povećala je količinu<br />
Fe u jetri 2 puta. Međutim, porast količine Fe u timusu<br />
izazvan je isključivo masnoćama u hrani. Sadržaj Cu u jetri<br />
blago se smanjio u skupini FCO. U slezeni je porast količine<br />
Fe izazvan prehranom obogaćenom Fe negativno<br />
korelirao s količinom Cu. Na antiokidacijski status utjecali<br />
su oboje, i prehrambena masnoća i Fe. Miševi čija je hrana<br />
bila obogaćena kukuruznim uljem imali su povećanu količinu<br />
TBARS, s većim porastom u skupini FCOFe. Aktivnost<br />
CuZnSOD u jetri smanjena je u skupini FCO, dok je dodatak<br />
Fe u prehrani uzrokovao daljnje smanjenje aktivnosti<br />
enzima. Rezultati ukazuju na to da prehrana obogaćena<br />
kukuruznim uljem uzrokuje oksidacijsko oštećenje i smanjenje<br />
antioksidacijske enzimske zaštite. Dodatak velikih<br />
količina Fe dodatno je utjecao na antioksidacijski sustav<br />
povećavajući osjetljivost jetrenog tkiva na lipidnu peroksidaciju.<br />
Uz to, prehrana obogaćena kukuruznim uljem i<br />
Fe povećala je u miševa potrebe za Cu.<br />
E-mail: robertd@medri.hr<br />
P23-2<br />
Povezanost koncentracije serumskog<br />
kolesterola i suicidnog ponašanja<br />
Ruljančić N, Malić A, Mihanović M<br />
Psihijatrijska bolnica Sveti Ivan, Zagreb, Hrvatska<br />
Ranije studije pokazale su povezanost niske koncentracije<br />
ukupnog kolesterola i suicidnog ponašanja. Cilj ispitivanja<br />
bio je ustanoviti postoje li razlike u koncentraciji serumskog<br />
kolesterola u bolesnika s određenim psihijatrij-<br />
<strong>Biochemia</strong> <strong>Medica</strong> 2006;16(Suppl 1):S1–S268<br />
S230<br />
P23-2<br />
Other<br />
and deleterious reactions such as peroxidation of polyunsaturated<br />
fatty acids and decreased antioxidant enzyme<br />
activities. The mechanism by which Fe is involved in<br />
initiating or promoting oxidative damage is not entirely<br />
clear. The aim of the study was to analyze the eff ect of<br />
high dietary iron (Fe) on liver antioxidant status in mice<br />
fed corn oil-enriched diet. Male Balb/c mice were fed for<br />
3 weeks standard diets enriched with 5% by weight of<br />
corn oil, with adequate Fe (FCO group), or supplemented<br />
with 1% carbonyl Fe (FCOFe group). Control group was<br />
fed standard diet. Fe, copper (Cu), and zinc (Zn) levels<br />
in liver tissue were determined by inductively coupled<br />
plasma spectrometry. Cu/Zn superoxide dismutase<br />
(CuZnSOD) and glutathione peroxidase (GPx) activities<br />
were determined spectrophotometrically in liver homogenate<br />
supernatant. Lipid peroxidation was evaluated by<br />
measuring thiobarbituric acid-reactive species (TBARS).<br />
High-Fe diet induced 2-fold increase of hepatic Fe level.<br />
However, the increase of thymic Fe level was induced<br />
solely by dietary fat. Hepatic Cu level slightly decreased<br />
on FCO diet. In spleen, the high-Fe diet induced increase<br />
of Fe level correlated negatively with Cu level. The antioxidant<br />
status was infl uenced by both dietary fat and Fe.<br />
Mice fed corn oil-enriched diets had a higher concentration<br />
of TBARS, with a greater increase on FCOFe diet.<br />
Hepatic CuZnSOD activity was decreased in FCO diet,<br />
and Fe supplementation caused further decrease in the<br />
enzyme activity. These results suggest that feeding with<br />
corn oil-enriched diets increases oxidative damage by<br />
decreasing antioxidant enzyme defense. The high-Fe<br />
diet additionally aff ects the antioxidant defense system,<br />
further increasing the tissue susceptibility to lipid peroxidation.<br />
Additionally, both corn oil- and Fe-enriched diets<br />
increased Cu requirements in mice.<br />
E-mail: robertd@medri.hr<br />
Relationship between serum cholesterol and<br />
suicidal behavior<br />
Ruljančić N, Malić A, Mihanović M<br />
Sveti Ivan Psychiatric Hospital, Zagreb, Croatia<br />
Previous studies have pointed to a relationship between<br />
low level of total serum cholesterol and suicidal behavior.<br />
The aim of our study was to investigate the possible differences<br />
in cholesterol level between psychiatric patients
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