Posterske teme Poster topics - Biochemia Medica

P3-4 Koagulacija
laboratorijski obrađeno 197 ispitanika zbog sumnje na
poremećaj primarne hemostaze, te je napravljena korelacija
PFA-100 s VWF:RCo (r=-0,5091 za CEPI; r=-0,5649 za
CADP) i s VWF:Ag (r=-0,4003 za CEPI; r=-0,5223 za CADP).
Logaritamskom transformacijom dobivena je jača korelacija
s VWF:RCo (r=-0,6423 za CEPI; r=-0,7403 za CADP), kao
i s VWF:Ag (r=-0,4879 za CEPI; r=-0,6117 za CADP). Ispitivanjem
korelacije vremena krvarenja po Ivyju s PFA-100
dobiveni su bolji rezultati (r=-0,4291 za CEPI; r=-0,5614 za
CADP) nego s VWF (r=-0,2216 za VWF:RCo; r=-0,1590 za
VWF:Ag). Potpuno podudaranje rezultata između PFA-100
i ispitivanja PA (oba rezultata patološka ili oba normalna)
dobiveno je u 94/162 (58%) ispitanika. U skupini od 96 ispitanika
s normalnim nalazom PA, u 72 (75%) je dobiven
normalan rezultat za CEPI-CT i CADP-CT, u 13 (13,5%) ispitanika
dobiven je barem jedan patološki nalaz na PFA-
100, dok su oba patološka rezultata dobivena u 11 (11,5%)
ispitanika. Od 66 ispitanika s patološkim vrijednostima PA
s najmanje jednim agonistom, oba patološka rezultata na
PFA-100 nađena su u 22 (33,3%) ispitanika, barem jedan
normalan rezultat dobiven je u 15 (22,7%) ispitanika, a oba
normalna nalaza su dobivena u 29 (44,0%) ispitanika. U
zaključku, normalan nalaz CEPI-CT i CADP-CT ne može isključiti
sve poremećaje primarne hemostaze zbog izrazite
složenosti primarne hemostaze te velikog broja različitih
poremećaja funkcije trombocita.
E-mail: marijamilos1@yahoo.com
P3-5
Predviđanje stupnja defi cita FVIII analizom
reakcijskih krivulja za APTV
Miloš M, Coen-Herak D, Zadro R
Klinički zavod za laboratorijsku dijagnostiku, Klinički bolnički centar
Zagreb, Zagreb, Hrvatska
Na modernim koagulacijskim analizatorima APTV ne
predstavlja samo broj, nego skup foto-optičkih podataka
u obliku reakcijske krivulje. U ovom radu napravljena je
kvantitativna analiza reakcijskih krivulja za APTV na analizatoru
Behring Coagulation Timer (BCT) te je pomoću
dobivenih parametara ispitana mogućnost predviđanja
stupnja defi cita FVIII. U uzorcima plazme 38 zdravih ispitanika
i 87 bolesnika s hemofi lijom A izmjeren je APTV (Actin
FS) uporabom dviju metoda procjene reakcijske krivulje:
zadana promjena apsorpcije (fi x e d a b s o r b a n c e , APTV-FA) i
točka infl eksije (point of infl exion, APTV-PI). Zatim su izračunati
slijedeći parametri: delta-APTV (DaPTT) kao razli-
Biochemia Medica 2006;16(Suppl 1):S1–S268
S108
was found to be 80-160 s for CEPI-CT, and 60-120 s for
CADP-CT. The PFA-100 CTs were correlated to VWF:RCo
(r=-0.5091 for CEPI; r=-0.5649 for CADP), and to VWF:Ag
(r=-0.4003 for CEPI; r=-0.5223 for CADP). With logarithmic
transformation of data, we obtained an even stronger
correlation to VWF:RCo (r=-0.6423 for CEPI; r=-0.7403 for
CADP), as well as to VWF:Ag (r=-0.4879 for CEPI; r=-0.6117
for CADP). Bleeding times were correlated much better to
PFA-100 CTs (r=0.4291 for CEPI; r=0.5614 for CADP) than
to plasma VWF levels (r=-0.2216 for VWF:RCo; r=-0.1590
for VWF:Ag). The overall agreement (both normal or both
abnormal) between PFA-100 and PA was 94/162 (58%).
In the group of 96 patients with normal PA results, both
normal CTs were found in 72 (75%), at least one abnormal
CT in 13 (13.5%), and both abnormal CTs in 11 (11.5%) patients.
Among 66 patients with at least one abnormal PA
result, both abnormal CTs were observed in 22 (33.3%), at
least one normal CT in 15 (22.7%) and both normal CTs in
29 (44.0%) patients. In conclusion, normal CTs could not
exclude all primary hemostasis disorders, probably due
to the large number and variety of platelet defects.
E-mail: marijamilos1@yahoo.com
P3-5
Is it possible to predict the degree of FVIII
defi ciency from aPTT waveform analysis on a
Behring Coagulation Timer (BCT)?
Miloš M, Coen-Herak D, Zadro R
Coagulation
Clinical Institute of Laboratory Diagnosis, Zagreb University Hospital
Center, Zagreb, Croatia
With modern coagulation instruments, every aPTT result
is not just a number but a collection of photo-optical
data in the form of reaction curve. We performed a quantitative
aPTT waveform analysis to see whether it was
possible to detect the degree of FVIII defi ciency using parameters
from this analysis. We measured aPTT (Actin FS)
in 38 normal subjects and 87 hemophilia A patients on a
Behring Coagulation Timer, with two diff erent evaluation
modes: fi xed absorbance (FA) and point of infl exion (PI).
Additionally, we calculated delta-aPTT (DaPTT) as aPTT-PI
minus aPTT-FA and aPTT-slope ratio (aPTTSR) as the ratio
between DaPTT and aPTT-FA. FVIII activity was measured