Posterske teme Poster topics - Biochemia Medica
Posterske teme Poster topics - Biochemia Medica
Posterske teme Poster topics - Biochemia Medica
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P19-7 Pedijatrijska laboratorijska dijagnostika<br />
na ženu heterozigota za ALD. U zaključku, metoda<br />
određivanja koncentracije MKVDL u serumu omogućila<br />
je probiranje širokog raspona rizičnih osoba u svrhu otkrivanja<br />
asimptomatskih bolesnika s X-ALD. Naglašava se<br />
potreba mjerenja MKVDL u serumu svih članova ne samo<br />
uže, već i šire obitelji bolesnika s ALD, kako bi se otkrili<br />
i drugi bolesnici i/ili heterozigoti za ALD. Otkrivanje heterozigota<br />
otvara mogućnost za prevenciju bolesti kroz<br />
gensko savjetovanje. Normalne koncentracije MKVDL u<br />
serumu u klinički suspektnih ispitanica ne isključuju heterozigotnost.<br />
U takvim slučajevima potrebno je odrediti<br />
koncentraciju MKVDL u homogenatu kulture kožnih fi -<br />
broblasta te eventualno napraviti mutacijske analize gena<br />
ABCD1.<br />
E-mail: kbilic@kbc-zagreb.hr<br />
P19-8<br />
Usporedba reagensa dvaju proizvođača<br />
za određivanje vrijednosti C-reaktivnog<br />
proteina u nedonoščadi i novorođenčadi<br />
Vukelić N, Vukasović I, Topić E<br />
Klinički zavod za kemiju, KB Sestre milosrdnice, Zagreb, Hrvatska<br />
C-reaktivni protein (CRP) je reaktant akutne faze koji je u<br />
zdravih osoba prisutan u niskim koncentracijama. Patološka<br />
stanja kao što su bakterijske infekcije, upale ili razaranje<br />
tkiva praćena su povećanjem vrijednosti CRP uslijed<br />
otpuštanja upalnih citokina. Određivanje vrijednosti CRP<br />
vrlo je važno u dijagnostici i praćenju infekcije u nedonoščadi<br />
i novorođenčadi. U novorođenčadi sepsa može<br />
početi nespecifi čnim simptomima i dijagnosticira se tek<br />
na osnovi patoloških laboratorijskih nalaza. Cilj rada bio je<br />
ispitati analitičku pouzdanost i reproducibilnost reagensa<br />
Full Range CRP tvrtke Randox za određivanje vrijednosti<br />
CRP u serumu/plazmi (mjerno područje 0,1-160,0 mg/L) u<br />
usporedbi s CRP reagensima tvrtke Olympus kao referentnim<br />
reagensima: Olympus CRP reagensom za koncentracijsko<br />
područje 5,0-300,0 mg/L i Olympus CRP Latex<br />
reagensom namijenjenom ranoj dijagnozi infekcije u nedonoščadi<br />
i novorođenčadi. Za Olympus CRP Latex reagens<br />
postoje dvije aplikacije: osjetljiva (SCRP, za koncentracijsko<br />
područje 0,5-20,0 mg/L ) i visoko osjetljiva (HS-<br />
CRP, za koncentracijsko područje 0,05-2,00 mg/L). Za sve<br />
reagense primijenjene su izvorne baždarne i kontrolne<br />
otopine, kao i aplikacije za instrument Olympus AU 640.<br />
Ispitivanja su provedena u skladu s preporukama ECCLS.<br />
Diagnostic tests should be off ered to all at risk relatives<br />
of X-ALD patients and should include members of the<br />
extended family. Identifi cation of heterozygotes provides<br />
an opportunity for disease prevention through genetic<br />
counseling. Normal concentration VLCFAs in clinically<br />
suspected females does not exclude carrier state. In these<br />
cases, the combined use of fi broblast VLCFA level analysis<br />
and possibly ABCD1 gene mutation analysis could detect<br />
X-ALD carriers correctly.<br />
E-mail: kbilic@kbc-zagreb.hr<br />
Pediatric laboratory diagnostics<br />
P19-8<br />
Comparison of reagents from two<br />
manufacturers for C-reactive protein<br />
determination in preterm and term neonates<br />
Vukelić N, Vukasović I, Topić E<br />
University Department of Chemistry, Sestre milosrdnice University<br />
Hospital, Zagreb, Croatia<br />
C-reactive protein (CRP) is an acute phase reactant, which<br />
is found in low concentrations in healthy individuals.<br />
Pathologic states such as bacterial infection, infl ammation<br />
or tissue lesions are associated with an increase in<br />
CRP level due to the release of infl ammatory cytokines.<br />
Determination of CRP is of utmost importance in the diagnosis<br />
and monitoring of infection in preterm and term<br />
neonates. In neonates, sepsis may begin with unspecifi c<br />
symptoms and is only diagnosed on the basis of pathologic<br />
laboratory fi ndings. The aim of the study was to assess<br />
analytical reliability and reproducibility of the Full Range<br />
CRP reagent (Randox) for CRP determination in serum/<br />
plasma (measuring range 0.1-160.0 mg/L) in comparison<br />
with Olympus CRP reagents as reference reagents: Olympus<br />
CRP reagent for the concentration range of 5.0-300.0<br />
mg/L and Olympus CRP Latex reagent intended for early<br />
diagnosis of infection in preterm and term newborns. The<br />
Olympus CRP Latex reagent has two applications: sensitive<br />
(SCRP, for concentration range of 0.5-20.0 mg/L) and<br />
highly sensitive (HSCRP, for concentration range of 0.05-<br />
2.00 mg/L). The original calibrated and control solutions<br />
and applications for the Olympus AU 640 instrument were<br />
used for all reagents. Tests were performed in line with<br />
<strong>Biochemia</strong> <strong>Medica</strong> 2006;16(Suppl 1):S1–S268<br />
S211
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