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Neonicotinoid Pesticides and Bees - The Food and Environment ...

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Compound tested<br />

Species<br />

Imidacloprid<br />

(Gaucho, 70% a.i.)<br />

Apis melliferea<br />

ligustica L. x A. m.<br />

caucasica L.<br />

Imidacloprid<br />

(Gaucho, 70% a.i.)<br />

‘Honeybees’<br />

Study type Test dose Test<br />

duration<br />

Semi-field/oral<br />

exposure/adults<br />

Semi-field/oral<br />

exposure/adults<br />

0, 0.35, 0.7 mg<br />

a.i./seed<br />

(in tunnels)<br />

0.35, 0.7, 1.05<br />

mg a.i./seed<br />

(in tunnels)<br />

0.7 mg<br />

a.i./seed (in<br />

field)<br />

20ppb spiked<br />

sugar solution<br />

(feeding<br />

experiments<br />

under field<br />

conditions)<br />

Endpoints Results Notes (reference<br />

to study numbers<br />

in figures 20 <strong>and</strong><br />

21)<br />

- Behaviour,<br />

activity, flower<br />

foraging,<br />

progress of<br />

pollination,<br />

collection of<br />

nectar.<br />

- Vitality, foraging<br />

activity,<br />

behaviour<br />

(control)<br />

No effects observed on<br />

any of the measures<br />

No effects observed on<br />

any of the measures.<br />

No residues of<br />

imidacloprid found in<br />

sunflower nectar (LOQ<br />

10ppb)<br />

<strong>Neonicotinoid</strong> pesticides <strong>and</strong> bees Page 80 of 133<br />

Report to Syngenta Ltd<br />

Reference<br />

In french Ambolet et al<br />

(1997)<br />

In french Ambolet et al<br />

(1999)<br />

Table 17: Overview of semi-field <strong>and</strong> field studies of the effects of sublethal <strong>and</strong> chronic exposure to clothianidin <strong>and</strong> thiamethoxam on Apis bees<br />

Compound tested Study type Test dose Test Endpoints Results Notes Reference<br />

Species<br />

duration<br />

Thiamethoxam Dosed in field 1.34ng in 20<br />

µl/bee<br />

Clothianidin<br />

(powder form)<br />

Dosed in field /<br />

oral exposure<br />

0.05, 0.5, 1,<br />

2ng/bee<br />

3 days after<br />

dosing<br />

Immediately<br />

after<br />

Return to hive Significant reduction in<br />

numbers of treated<br />

foragers returning<br />

particularly from<br />

Foraging<br />

behaviour:<br />

unfamiliar l<strong>and</strong>scapes<br />

At 1ng/bee 73.8%<br />

returned to the hive<br />

(5) Henry et al<br />

2012<br />

(6) Schneider et al<br />

(2012)

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