Neonicotinoid Pesticides and Bees - The Food and Environment ...

Compound tested
Species
Imidacloprid
Apis mellifera
Imidacloprid
(Technical >97%)
+ metabolites
Study type Test dose Test
duration
Lab study / contact
exposure / adults
Lab study / oral
acute & chronic
exposures / adults
(ii) 1, 10
ng/bee 5hydroxyimidacloprid
1µl at 1.25,
2.5, 5, 10, 20
ng/bee
0.1, 1, 10 µg/L
for 10 days
and
(ii) 1h after
application
0, 15, 30
and 60 mins
after
treatment
10 days
8 days
Endpoints Results Notes (study
reference in figure
(i) Gustatory
function
(ii) Motor activity
(iii) Nonassociative
learning abilities
(PER)
Mortality; hyperresponsiveness,
hyperactivity,
required (not significant at
0.1ng/bee) while olefin
significantly increased the
number of trials required
as in 7 d old bees(also at
0.1ng/bee).
(ii) Significant increase in
number of trials required
at both doses (suggests
that effect not due tohydroxy-imidacloprid
but
its metabolites – most
likely olefin).
(i) 5 ng/bee and above
had a significant effect on
the gustatory function
(ii) 2.5ng/bee and above
significantly impaired
motor activity in open field
(these effects are
amplified over time).
Motor activity was
significantly enhanced at
1.25ng/bee
(iii) PER habituation was
significantly facilitated at
doses below 2.5 ng/bee
50 % mortality after 8
days
Neonicotinoid pesticides and bees Page 53 of 133
Report to Syngenta Ltd
20)
Reference
(5) Lambin et al
(2001)
(6) Suchail et al
(2001)