semmelweis university 2 0 1 2 / 2 0 1 3

SEMMELWEIS UNIVERSITY / FACULTY OF PHARMACY
476
Among the biomedical problems some metaldependent diseases (e.g. Wilson’s desease, Menkes’
desease) are touched upon. Much time will be devoted to metal complexes of antitumor activity
and to those that are used in the treatment of rheumathoid artritis.
Metal ion toxicity, an increasing hazard due to the contaminated environment, and chelate
therapy are the topics that also deserve attention.
A look into the future closes the course.
BIOTECHNOLOGY
Department of Plant Anatomy (ELTE)
Topics
1. Introduction to biotechnology
Historical evolution of biotechnology. Biotechnology as an interdisciplinary pursuit.
Application of biotechnology. Substrates of biotechnology.
2. Applied genetics
Selection and screening. Culture maintenance. Mutagenesis. Protoplast and cell fusion
technologies.
3. Introduction to genetic engineering
The emergence of recombinant DNA technology. Regulation and control of recombinant
DNA experimentation. Basic recombinant DNA techniques. Restriction endonucleases and
other DNA and RNA modification enzymes.
4. Gene cloning
Transformation. Cloning vectors. Construction of libraries. Indentification of transformants
and recombinants. Gene isolation and analysis. Nucleic acid labelling and detection.
Hybridization analysis. DNA sequencing. DNA amplification by the polymerase chain
reaction.
5. Expression of foreign DNA in bacteria
Control of gene expression in bacteria and in eukaryotes. Expression of eukaryotic genes in
bacteria. Alternative host organisms.
6. Cloning in higher organisms
Gene cloning in mammalian cells. Vector systems. Gene transfer methods. Plant tissue
culture techniques. Rotoplast fusion. Plant genetic engineering. Transformation using.
Agrobacterium as a gene vector. Direct gene transfer. Crop improvement using molecular
techniques: resistance to herbicides and diseases. RFLP-analysis.
7. Application of molecular biology in medicine
Diagnosis of genetic disorders. DNA fingerprinting. Case applications.
8. Genetic engineering and the pharmaceutical industry
Fermentation products. Antibiotics production. Protein production. Recombinant virus
vaccines. Monoclonal antibodies.
9. Fermentation technology
Microbial cultivation. Bioreactor design. Media design. Instrumentation and process
control. Scale-up. Animal and plant cell cultivation. Solid substrate fermentation.
10. Enzyme technology
Enzyme engineering. Site-directed mutagenesis. Enzyme stabilization. Product of enzymes.
Immobilized enzymes. Immobilization of cells.
11. Biosensors
Priciple and structure of biosensors. Application in clinical chemistry and medicine.
Application in fermentation industries.