semmelweis university 2 0 1 2 / 2 0 1 3

SEMMELWEIS UNIVERSITY / FACULTY OF PHARMACY
438
BIOCHEMISTRY
Dept. of Medical Biochemistry
Lecturer: Dr. György Szabados
Tutor: Dr. Attila Ambrus
First Semester
Lectures (2 hours per week) Practicals
Lipid metabolism: Intestinal absorption of lipids.
Plasma lipoproteins and lipid transport. Outline
of triacyl glycerol metabolism. Regulation of lipid
metabolism: lipogenesis and lipolysis.
Metabolism of steroid hormones. Seminar: Lipoprotein
lipase and carnitine
deficiency
Metabolism of steroid hormones.
Matabolism of arachidonic acid, eicosanoids.
Nutritionally essential amino acids. Interconversion.
of amino acids: transamination. Overall aspects of
amino acid metabolism.
Amino acid catabolism. Fate of nitrogen: Seminar: Pyruvate
Deamination. dehydrogenase
Elimination of ammonia: The urea cycle. Biosynthesis and pyruvate
of nonessential amino acids. carboxylase deficiency
Metabolic fates of individual amino acids: glycogenic
and ketogenic amino acids. Amino acid decarboxylation:
biogen amines.
Nucleotide metabolism: biosynthesis and catabolism of Seminar: Liver failure
purine and pyrimidine nucleotides.
Physical properties of DNA. Structure of DNA Midterm: lipids and
(A, B, Z-DNA). amino acids
Topoisomerases. Supercoiling of DNA. The structure of
nucleosome. Replication in procaryotes.
DNA polymerases. Okazaki fragments. DNA ligase.
The mechanism of replication. Origin of replication.
Replication of eukaryotic cells. The cell cycle. DNA
polymerase alpha, beta, gamma and delta. Mutations,
Ames test. The repair processes. Excision repair of
UV-induced thymine-thymine dimmers.
The different types of RNAs. Prokaryotic transcription. Seminar: Vitami B12
The role of sigma factor. The operon model. The lac and folate deficiency
and trp operons. Eukaryotic replication. Transcription
factors. Enhancers. The maturation of the different
RNAs. Splicing.
Translation. The genetic code. Initiation, elongation
and termination. Signals for protein sorting.
Biochemical preparations in therapy. Biotechnological Midtern: nucleotides
methods for drug preparation. Recombinant DNA and macromolecules
technology. Therapy of the diseases (inherited enzymopathies)
by biochemical preparations.