semmelweis university 2 0 1 2 / 2 0 1 3

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SEMMELWEIS UNIVERSITY / FACULTY OF MEDICINE
MEDICAL BIOCHEMISTRY, MOLECULAR
AND CELL BIOLOGY II.
Teaching secretary: István Léránt Ph.D.
Fall semester
Schedule of lectures
1. Absorption of carbohydrates. Glucose carriers and transporters.
2. Glycolysis; entry of other hexoses into glycolysis
3.
Pyruvate dehydrogenase. Citric acid cycle. Absorption of lipids. Oxidation of fatty acids. Metabolism of
ketone bodies. Digestion of proteins. NADH and amino acid metabolism
4. Oxidative phosphorylation.
5. Gluconeogenesis, gluconeogenesis in starvation
6.
Regulation of blood sugar level. Glycogen mobilization in liver and muscle. Glucagon. Secretion of insulin,
insulin receptors, influence of insulin on various tissues
7. Biochemistry of diabetes mellitus, type I and II diabetes.
8. Synthesis of fatty acids. Biosynthesis of triacylglycerols and phospholipids. Lipid transport.
9. Metabolism and transport of cholesterol. Bile acids.
10. Degradation of proteins, catabolism of amino acids I.
11. Catabolism of amino acids II.. Metabolism of porphyrines
12. Structure and function of nucleotides. Metabolism of nucleotides.
13.
14.
Metabolism of red blood cells. Metabolism of striated and heart muscle. The role of SREBP, PPAR-alpha
and beta in muscle metabolism.
Intermediary metabolism of kidneys, Regulation of intermediary metabolism during fasting and in the
well-fed state. ChREBP signal transduction.
Fall semester
Schedule of laboratory lessons and seminars
1. Safety rules for work in the laboratory. & Amidolytic activity of trypsin.
2. Enzyme kinetics – computer simulation
3. Competitive inhibition of succinate dehydrogenase (laboratory lesson)
4. Gene therapy – stem cell therapy (consultation)
5. Oxidative phosphorylation in mitochondria (laboratory lesson)
6. Lactic acidosis (consultation)
7. Allosteric regulation of pyruvate kinase. (laboratory lesson)
8. Fructose intolerance, & McArdle’s disease (consultation)
9. Digestion of lipids, role of bile acids (laboratory lesson)
10. Signal transduction of insulin (consultation)
11. Determination of serum cholesterol and triglyceride levels
12. Lipoprotein lipase, & carnitine deficiency (consultation)
13.
Purification of trypsin by affinity chromatography. & Substrate specificity of trypsin and chymotrypsin.
(laboratory lesson)
14. Hereditary hyperammonemias. & Vitamin B12 deficiency, methylmalonic acidemia (consultation)