GHS Classification Guidance for the Japanese Government

decision, judgement by expert’s shall be sought for a final decision.
For individual species, see below. As for data in List 1 without indication of
accordance with the test guidelines below and other relevant criteria, such data shall be
adopted in which organism species, exposure time, and endpoint each accord with those
stipulated in the test guidelines and other relevant criteria.
■ Fish:
Chronic or long-term toxicity tests using fish shall be conducted according to OECD
Test Guideline 210 (Fish early life stage test), Fish Life Cycle Test(US EPA 850.1500), or
corresponding test methods(one- or two-generation test). Although OECD Test Guideline
210 is designed for sub-chronic toxicity, its test results may be utilized as indexes of
chronic aquatic toxicity since they provide good indexes for chronic toxicity, .
The appendix to OECD Test guideline 210 defines exposure time for each species (for
example, the case of Oryziatidae, up to 30 days (at minimum 28 days) after hatching),
while a Fish Life Cycle Test(US EPA850.1500) provides no definition on the duration.
Accordingly, for data requiring confirmed reliability, the exposure time adopted is
considered to be appropriate if the compliance to OECD Test Guideline 210, the Fish Life
Cycle Test, or corresponding test methods is clearly noted.
Endpoints are based on hatching success rate, growth (change in length and weight),
spawning success rate, and survival rate.
■ Crustacea:
Chronic toxicity tests using crustacea shall be conducted in accordance with OECD Test
Guideline 211 (Daphnia reproduction), US EPA OPPTS 850.1035 (Mysidacea chronic
toxicity), or corresponding test methods (NOECs of 21 days for Daphnia, NOECs of 7
days or more for Ceriodaphnia).
Endpoints are based on the period up to the first spawning, number of eggs per female,
growth, and survival rate.
■ Algae (or other aquatic plants):
・Algae: Since OECD Test Guideline 201 (Algae growth inhibition test, 72 or 96
hours) is not a long-term test, its NOECs are not generally used as evidence for
exclusion from chronic aquatic toxicity. Yet, when the classification as acute aquatic
toxicity is based on test result on a single species of algae or another aquatic plant, and
when NOECs exceed 1 mg/L for other species of algae, NOECs may be used as
evidence for exclusion.
In principle, growth inhibition (NOEC) by growth rate method is used for an
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