E. Coli

E. Coli E. Coli

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Review of Literature majority of 276 isolates (26.2%) of salmonella species were recovered from heart, liver and intestine of the broilers and layers. S.entertidis (85 isolates, 98.8%), S.virchow (48 isolated, 57.8%) S. paratyphi (41 isolates, 57.71%) and S.infants (30 isolates, 20.6%) were distributed in poultry and poultry environment. Mohammed et al. (1999) collected 200 faecal samples from living chickens in addition to 180 samples collected from chicken environment, Litter (75), feed (30) and water (75) at Kafr-El-Sheikh province. The total incidence of salmonella was 2.5% in chicken, 5.33% in Litter, 3.33% in feed and 2.66% in water. The number of isolated S.entertidis strains from litter was (1) and feed (1), mean while S.typhimurium was isolated from chicken (2), S.anatum were isolated from chicken (3) and S.pullorun were isolated from chicken and water (1 each in number). Hatab (2001) collected 25 poultry feed samples from poultry houses at different localities in Dakahila province. Salmonella species were isolated from 3 raw mash samples with an incidence of 12%. Sertoyping of the three isolated Salmonella species revealed that 2 strains were S. typhimurium and one untypable strain. Mohamad (2002) illustrated the occurrence of Salmonella species in 31 poultry manure samples in federal Germany with a frequency of 2/31 (6%). Both isolates were serotyped as S.agona. Salmonella species was counted in poultry manure with mean number of 162 cell per gram manure. 14

Review of Literature Moreno et al. (2007) mentioned that antimicrobial resistance is an increasing phenomenon but its quantitative estimation remains controversial. The classical resistance percentage approach is not well studied to detect either emergence or low levels resistance. They performed E.coli enumeration in facial samples of broilers (82 pooled samples). Antimicrobial susceptibility of isolates was supplemented with 1 µg/ml of cefotaxime for E. coli detection, 93% (76/82) of broiler pooled samples tested positive. 2.1.3. Frequent Enterobacteriaceae in broiler and surrounding environment. Verma and Adlakha (1971) stated that, out of 359 chickens suffered from pneumonia, sepcticemia, egg peritonitis, enteritis and persistent yolk sac. Klebsiella species were isolated from 12 chickens. Proteus species were isolated from 4 and paracolon bacteria from one. Karim and Ali (1976) examined 200 dead chick embryos of 22 days old. Proteus species were isolated from dead chickens embryos. Sarakbi (1979) reported the incidence of Klebsiella species in different organs of ill and dead one day-old chicks. A total of 438 samples from yolk-sac, heart blood, liver (146 each) were examined for the incidence of Klebsiella species, and result in recovery rates of 17 (11.64), 10 (6.85%) and 10 (6.85%), respectively. K. pneumoniae was identified in 10, 7, 7 strains recovered from yolk sac, heart blood and liver samples respectively while K.ozoenae was isolated from two yolk sac samples. 15

Review of Literature<br />

Moreno et al. (2007) mentioned that antimicrobial resistance is<br />

an increasing phenomenon but its quantitative estimation remains<br />

controversial. The classical resistance percentage approach is not well<br />

studied to detect either emergence or low levels resistance. They<br />

performed E.coli enumeration in facial samples of broilers (82 pooled<br />

samples). Antimicrobial susceptibility of isolates was supplemented<br />

with 1 µg/ml of cefotaxime for E. coli detection, 93% (76/82) of<br />

broiler pooled samples tested positive.<br />

2.1.3. Frequent Enterobacteriaceae in broiler and surrounding<br />

environment.<br />

Verma and Adlakha (1971) stated that, out of 359 chickens<br />

suffered from pneumonia, sepcticemia, egg peritonitis, enteritis and<br />

persistent yolk sac. Klebsiella species were isolated from 12 chickens.<br />

Proteus species were isolated from 4 and paracolon bacteria from one.<br />

Karim and Ali (1976) examined 200 dead chick embryos of 22<br />

days old. Proteus species were isolated from dead chickens embryos.<br />

Sarakbi (1979) reported the incidence of Klebsiella species in<br />

different organs of ill and dead one day-old chicks. A total of 438<br />

samples from yolk-sac, heart blood, liver (146 each) were examined<br />

for the incidence of Klebsiella species, and result in recovery rates of<br />

17 (11.64), 10 (6.85%) and 10 (6.85%), respectively. K. pneumoniae<br />

was identified in 10, 7, 7 strains recovered from yolk sac, heart blood<br />

and liver samples respectively while K.ozoenae was isolated from two<br />

yolk sac samples.<br />

15

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