E. Coli

List of contents
Page
1-Introduction………………………………..…………….1
2-Review of literature
2.1. Incidence of pathogenic Enterbacteriaceae…………….…3
2.1.1. Different E. coli serotypes isolated from broilers…….3
2.1.2. Salmonella…………………………………………….9
2.1.3. Frequent of Enterobacteriaceae in broiler and
surrounding environment………………………..…………15
2.3. Pathogenic Enterbacteria in Human………………..….. 19
2.3.1. E. coli…………………………………………..……22
2.3.2. Salmonella……………………………………..….....27
3-Materials and Methods
3.1. Materials……………………………………….……....29
3.1.1. Samples:
3.1.2. Media used:
3.1.3. Media for biochemical reactions………….. ….….….31
3.1.4. Solutions and indicators in biochemical identification.34
3.1.6. Media used for antibiotic sensitivity test………….…..35
3.1.7.Diagnostic E.coli Antisera……………………….…....35
3.2. Methods
3.2.1. Collection of sample…………………………….……38
3.2.2. Isolation of Enterbacteriaceae from organ samples…...38
3.2.3. Bacteriological identification………………………....39
3.2.4. Biochemical characterization………………………....39
3.2.5. Serological typing of the isolated E. coli…………..…41
3.2.6. Antibiotic sensitivity test………………………………42