E. Coli
E. Coli
E. Coli
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3. The plate was swabbed in different directions to wet the whole of its<br />
surface<br />
4. Excessive fluid was discarded by its pipetting then plates were dried<br />
for up to 30 min.<br />
5. The chosen antibiotic discs were distributed to the surface of the plate<br />
with sterile point forceps, a gentle pressure over the discs was done to<br />
ensure full sticking of the discs to the medium then the plates were<br />
aerobically incubated at 37° C /24 hours.<br />
6. The degree of the sensitivity was determined by measuring the<br />
diameter of inhibition zone in mm produced by diffusion of antibiotics,<br />
from disc to surrounding medium.<br />
Table(1)Biochemical reactions,members of Enterobactericeae,<br />
Cruickshank et al., (1975).<br />
M.O.<br />
E.coli<br />
Indol<br />
M.R<br />
V.P.<br />
Citrate<br />
Urease<br />
Lactose<br />
Maltose<br />
Mannitol<br />
Sucrose<br />
TSI<br />
+ + - - - + + + d Y/Y/H2S-<br />
Salmonella - + - + - - + + - R/Y/H2S<br />
+<br />
Enterobacter<br />
aerogens<br />
- - + + - + + + + Y/Y/H2S-<br />
Citrobacter<br />
diversus<br />
+ + - + + d + + - Y/Y/H2S-<br />
Klebsiella<br />
pneumoniae<br />
- - + + + + + + + Y/Y/H2S-<br />
Edwadsiella<br />
tarda<br />
+ + - - - - + + + Y/Y/H2S-<br />
TSI=triple iron agar d=26-75%(positive), Y=yellow ,R=red<br />
42
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