E. Coli

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ﺾﻌﺒﻟ ﺎﻴﻠﻤﻌﻣ ﺔﻳﻮﻴﺤﻟا تادﺎﻀﻤﻟا ماﺪﺨﺘﺳا ﻰﻠﻋ ﺔﺳارد ةدﺪﻌﺘﻣ ردﺎﺼﻣ ﻦﻣ ﺔﻳﻮﻌﻤﻟا ﺎﻳﺮﺘﻜﺒﻟا ﺪﻤﺤﻣ ﻦﻣ ﺔﻣﺪﻘﻣ ﺔﻟﺎﺳر ﺔﻴﻄﻋ لدﺎﻋ ﺔﻤﻃﺎﻓ 2005 ﻖﻳزﺎﻗﺰﻟا ﺔﻌﻣﺎﺟ , مﻮﻠﻌﻟا ﺔﻴﻠآ , مﻮﻠﻌﻟا سﻮﻳرﻮﻟﺎﻜﺑ فاﺮﺷا ﺖﺤﺗ رﺎﻤﻋ ﺪﻤﺤﻣ ﺪﻤﺣا/. د. ا ﺎﻴﺟﻮﻟﻮﻴﺑوﺮﻜﻴﻤﻟا ذﺎﺘﺳا ﻖﻳزﺎﻗﺰﻟا ﺔﻌﻣﺎﺟ -ىﺮﻄﻴﺒﻟا ﺐﻄﻟا ﺔﻴﻠآ ﻢﻴﺣﺮﻟا ﺪﺒﻋ ىﺮﻜﺒﻟا ﺪﻤﺤﻣ /. د. ا ﺎﻴﺟﻮﻟﻮﻴﺑوﺮﻜﻴﻤﻟا ذﺎﺘﺳا ﻖﻳزﺎﻗﺰﻟا ﺔﻌﻣﺎﺟ -ىﺮﻄﻴﺒﻟا ﺐﻄﻟا ﺔﻴﻠآ ﻰآز ﺔﻠﻟا قزر دﺎﻤﻋ/. د. ا ﻰﺟﻮﻟﻮﻳﺮﺘﻜﺒﻟا ثﻮﺤﺑ ﺲﻴﺋر سﻮﻣﺎﺠﻟا ضاﺮﻣا ثﺎﺤﺑا ﻢﺴﻗ ﺲﻴﺋرو ةﺮهﺎﻘﻟا- ﻰﻗﺪﻟا-ناﻮﻴﺤﻟا ﺔﺤﺻ ثﻮﺤﺑ ﺪﻬﻌﻣ ﻰﻟا ﺔﻋﺎﻨﻤﻟاو تﺎﻳﺮﻄﻔﻟاو ﻰﺟﻮﻟﻮﻳﺮﺘﻜﺒﻟا ﻢﺴﻗ , ىﺮﻄﻴﺒﻟا ﺐﻄﻟا ﺔﻴﻠآ , ﻖﻳزﺎﻗﺰﻟا ﺔﻌﻣﺎﺟ ﻰﺟﻮﻟﻮﻳﺮﺘﻜﺒﻟا ﻰﻓ ﺮﻴﺘﺴﺟﺎﻤﻟا ﺔﺟرد ﻰﻠﻏ لﻮﺼﺤﻠﻟ ( 2008)
List of contents Page 1-Introduction………………………………..…………….1 2-Review of literature 2.1. Incidence of pathogenic Enterbacteriaceae…………….…3 2.1.1. Different E. coli serotypes isolated from broilers…….3 2.1.2. Salmonella…………………………………………….9 2.1.3. Frequent of Enterobacteriaceae in broiler and surrounding environment………………………..…………15 2.3. Pathogenic Enterbacteria in Human………………..….. 19 2.3.1. E. coli…………………………………………..……22 2.3.2. Salmonella……………………………………..….....27 3-Materials and Methods 3.1. Materials……………………………………….……....29 3.1.1. Samples: 3.1.2. Media used: 3.1.3. Media for biochemical reactions………….. ….….….31 3.1.4. Solutions and indicators in biochemical identification.34 3.1.6. Media used for antibiotic sensitivity test………….…..35 3.1.7.Diagnostic E.coli Antisera……………………….…....35 3.2. Methods 3.2.1. Collection of sample…………………………….……38 3.2.2. Isolation of Enterbacteriaceae from organ samples…...38 3.2.3. Bacteriological identification………………………....39 3.2.4. Biochemical characterization………………………....39 3.2.5. Serological typing of the isolated E. coli…………..…41 3.2.6. Antibiotic sensitivity test………………………………42
Page 1: A STUDY ON THE USE OF ANTIBIOTICS I
Page 5 and 6: Tables Page Table(1)Biochemical rea
Page 7 and 8: Acknowledgements First of all thank
Page 9 and 10: 2 Introduction certain serogroups,
Page 11 and 12: Sodium chloride 5.0 g/l Agar pH 7.3
Page 13 and 14: Sodium citrate, tribasic 2.0 g/l So
Page 15 and 16: 3.1.4.Solutions and indicators in b
Page 17 and 18: Polyvalent 4: O6 , O27 , O78 , O148
Page 19 and 20: serological identification accordin
Page 21 and 22: 6- Urease test The test determine t
Page 23 and 24: 3. The plate was swabbed in differe
Page 25 and 26: 2.REVIEW OF LITERATURE 2.1. Inciden
Page 27 and 28: Review of Literature suggested that
Page 29 and 30: Review of Literature K5 (37.5%), 15
Page 31 and 32: Review of Literature within Ireland
Page 33 and 34: Review of Literature salmonella spe
Page 35 and 36: Review of Literature Sasipreeyajan
Page 37 and 38: Review of Literature Moreno et al.
Page 39 and 40: Review of Literature Mohamad (1996)
Page 41 and 42: Review of Literature pencillin (68.
Page 43 and 44: Review of Literature El-Ghamdi et a
Page 45 and 46: Review of Literature serious ever l
Page 47 and 48: Review of Literature the time from
Page 49 and 50: Review of Literature isolates from
Page 51 and 52: 4.Results A total of 295 chicken sa
Page 53 and 54:
Table (4): Incidence of chicken Ent
Page 55 and 56:
Abolila 11 10/11(90.9%) 10/10(100%)
Page 57 and 58:
fermentation (Lactose, Maltose, Man
Page 59 and 60:
isolates were salmonella, pseudomon
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Fig.(3)Triple sugar iron agar(TSI),
Page 63 and 64:
Fig.(4).Citrate negative E.coli iso
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Fig.(6)An isolate was sensitive to
Page 67 and 68:
Farm Mansoura1 Omda1 Asher3 Asher4
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Gentamycin, Colistin, Erythromycin,
Page 71 and 72:
Trimethoprim), Colistin, Erythromyc
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(Sulpamethox-azole/ Trimethoprim),
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Table(10)Sensitivity pattern for is
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R = Resistant S = Sensitive CTX30 =
Page 79 and 80:
Table(12 )Sensitivity Pattern of is
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Doxycyclin, Erythromycin, were effe
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Fig.(8) Sensitivity percentages of
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Discussion Concerning bacteriologic
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al., (1997) reported that 10% of E.
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1/2(50%) Salmonella broiler isolate
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12/14(85.71%) E.coli isolates from
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streptomycin, meanwhile higher perc
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Two out of eleven(18.18%) E.coli is
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and Nguyen (1995) who reported mode
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Summary Bacterial diseases have lon
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epresented 105/138(76.08%) and 33/1
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100%, 100%, 63.63%, 63.63%, 63.63%,
Page 105:
3-Results of antibiogram on biotype
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a pediatric cohort in aperiurbab ar
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Bozorgmehri O., Fard M.H. and Gilan
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importance in some poultry farms. 7
Page 114 and 115:
Fisher M.E., Trampel D. W. and Grif
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Hui, A.K. and Das, R., (2000): Prev
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Kikuvi, G.M.; Ole-Mapenay, I.M.; Mi
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Machado J. and Bernardo F. (1990):
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Oxoid manual (1998): The oxoid manu
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and O 157 Serotypes from piglets an
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Van Donkersgoed J., Manninen K., Po
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ﻦﻴﺘﺴﻟﻮآ , ﻦﻴﺳﺎ
Page 130:
- She received her primary educatio
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E. Coli

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