E. Coli
E. Coli E. Coli
3.1.4.7. Nitrate reduction test reagents: Reagent A: -Naphthyl amine 5 g..m 5N acetic acid 30% 1 litter Reagent B: Sulfanilic acid 8 g. 3.1.5. Stains used: (1975). 5N acetic acid 30% 1 litter Modified Gram’s stain used as described by Cruickshank et al., 3.1.6. Media used for antibiotic sensitivity test 3.1.6.1. Media used: Mueller-Hinton agar (oxoid) was prepared according to Finegold and Martin (1982). 3.1.6.2. Antimicrobial sensitivity discs (Din, 1998): Antmicrobial susceptibility discs were obtained from BioMerieux as follows in table (2). 3.1.7.Diagnostic E.coli Antisera The isolates were identified serologically by diagnostic E.coli antisera for pathogenic types were used in this study. The diagnostic O sera 51 vials (polyvalent,8 vials and monovalent,43 vials (product code 312001) Polyvalent 1: O1 , O26 , O86A , O111 , O119 , O127A , O128 Polyvalent 2: O44 , O55 , O125 , O 126, O146 , O166 Polyvalent 3: O18 , O114 , O142 , O151 , O157 , O185 35
Polyvalent 4: O6 , O27 , O78 , O148 , O159 , O168 Polyvalent 5: O20 , O25 , O63 , O153 , O 167 Polyvalent 6: O8 , O15 , O115 , O169 Polyvalent 7: O28ac , O112AC , O124 , O136 , O144 Polyvalent 8: O29 , O143 , O152 , O164 36
- Page 1 and 2: A STUDY ON THE USE OF ANTIBIOTICS I
- Page 3 and 4: List of contents Page 1-Introductio
- Page 5 and 6: Tables Page Table(1)Biochemical rea
- Page 7 and 8: Acknowledgements First of all thank
- Page 9 and 10: 2 Introduction certain serogroups,
- Page 11 and 12: Sodium chloride 5.0 g/l Agar pH 7.3
- Page 13 and 14: Sodium citrate, tribasic 2.0 g/l So
- Page 15: 3.1.4.Solutions and indicators in b
- Page 19 and 20: serological identification accordin
- Page 21 and 22: 6- Urease test The test determine t
- Page 23 and 24: 3. The plate was swabbed in differe
- Page 25 and 26: 2.REVIEW OF LITERATURE 2.1. Inciden
- Page 27 and 28: Review of Literature suggested that
- Page 29 and 30: Review of Literature K5 (37.5%), 15
- Page 31 and 32: Review of Literature within Ireland
- Page 33 and 34: Review of Literature salmonella spe
- Page 35 and 36: Review of Literature Sasipreeyajan
- Page 37 and 38: Review of Literature Moreno et al.
- Page 39 and 40: Review of Literature Mohamad (1996)
- Page 41 and 42: Review of Literature pencillin (68.
- Page 43 and 44: Review of Literature El-Ghamdi et a
- Page 45 and 46: Review of Literature serious ever l
- Page 47 and 48: Review of Literature the time from
- Page 49 and 50: Review of Literature isolates from
- Page 51 and 52: 4.Results A total of 295 chicken sa
- Page 53 and 54: Table (4): Incidence of chicken Ent
- Page 55 and 56: Abolila 11 10/11(90.9%) 10/10(100%)
- Page 57 and 58: fermentation (Lactose, Maltose, Man
- Page 59 and 60: isolates were salmonella, pseudomon
- Page 61 and 62: Fig.(3)Triple sugar iron agar(TSI),
- Page 63 and 64: Fig.(4).Citrate negative E.coli iso
- Page 65 and 66: Fig.(6)An isolate was sensitive to
Polyvalent 4: O6 , O27 , O78 , O148 , O159 , O168<br />
Polyvalent 5: O20 , O25 , O63 , O153 , O 167<br />
Polyvalent 6: O8 , O15 , O115 , O169<br />
Polyvalent 7: O28ac , O112AC , O124 , O136 , O144<br />
Polyvalent 8: O29 , O143 , O152 , O164<br />
36
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