E. Coli

3.1.4.7. Nitrate reduction test reagents:
Reagent A: -Naphthyl amine 5 g..m
5N acetic acid 30% 1 litter
Reagent B: Sulfanilic acid 8 g.
3.1.5. Stains used:
(1975).
5N acetic acid 30% 1 litter
Modified Gram’s stain used as described by Cruickshank et al.,
3.1.6. Media used for antibiotic sensitivity test
3.1.6.1. Media used:
Mueller-Hinton agar (oxoid) was prepared according to Finegold
and Martin (1982).
3.1.6.2. Antimicrobial sensitivity discs (Din, 1998):
Antmicrobial susceptibility discs were obtained from BioMerieux
as follows in table (2).
3.1.7.Diagnostic E.coli Antisera
The isolates were identified serologically by diagnostic E.coli
antisera for pathogenic types were used in this study. The diagnostic O
sera 51 vials (polyvalent,8 vials and monovalent,43 vials (product code
312001)
Polyvalent 1: O1 , O26 , O86A , O111 , O119 , O127A , O128
Polyvalent 2: O44 , O55 , O125 , O 126, O146 , O166
Polyvalent 3: O18 , O114 , O142 , O151 , O157 , O185
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