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E. Coli

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Lactose 10.0 g/l<br />

Dipotassium hydrogen phosphate 2.0 g/l<br />

Eosin Y 4.0 g/l<br />

Methylene blue 0.065 g/l<br />

Agar<br />

pH 6.8<br />

15.0 g/l<br />

3.1.3.Media used for Biochemical examination:<br />

3.1.3.1. Peptone water media (oxoid)<br />

Peptone 10.0 g/l<br />

Sodium chloride<br />

pH 7.2<br />

5.0 g/l<br />

It used for detection of indole production but now better results<br />

can be obtained by the used of tryptone water (oxoid).<br />

3.1.3.2. Glucose-phosphate peptone water medium (Oxoid)<br />

Peptone 5.0 g/l<br />

Dextrose 5.0 g/l<br />

Phosphate buffer<br />

pH 7.5<br />

5.0 g/l<br />

This medium is used for methyl-red and Voges-Proskauer tests<br />

for differentiation of the coli-aerogenes group.<br />

3.1.3.3. Simmons citrate (Oxoid)<br />

Magnesium sulphate 0.2 g/l<br />

Ammonium dihydrogen phosphate 0.2 g/l<br />

Sodium ammonium phosphate 0.8 g/l<br />

31

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